LOCUS BC012876 899 bp mRNA linear HUM 26-APR-2019
DEFINITION Homo sapiens cDNA clone MGC:17259 IMAGE:4149333, complete cds.
ACCESSION BC012876
VERSION BC012876.3
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 899)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 899)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
REFERENCE 3 (bases 1 to 899)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (26-APR-2019) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK Sequence update by database staff to remove vector contamination
COMMENT On Apr 26, 2019 this sequence version replaced BC012876.2.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 19 Row: a Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: GenomeScan gene
prediction, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..899
/db_xref="H-InvDB:HIT000036008"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:17259 IMAGE:4149333"
/tissue_type="Brain, glioblastoma with EGFR amplification"
/clone_lib="NCI_CGAP_Brn64"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
CDS 26..736
/codon_start=1
/product="Unknown (protein for MGC:17259)"
/protein_id="AAH12876.1"
/translation="MAWSPLLLTLLAHCTGSWAQSVLAQPPSVSGAPGQTVTISCTGS
STNIGAGYAVHWYQQFPGAAPKVLIYGNYNRPSGVPDRFSGSKSGTSASLAITGLQAE
DEADYYCQSYDGSLSGSVFGAGTKVTVLGQPKANPTVTLFPPSSEELQANKATLVCLI
SDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQ
VTHEGSTVEKTVAPTECS"
misc_feature 131..355
/note="IGv; Region: Immunoglobulin V-Type"
/db_xref="CDD:smart00406"
misc_feature 482..706
/note="IGc1; Region: Immunoglobulin C-Type"
/db_xref="CDD:smart00407"
BASE COUNT 215 a 294 c 228 g 162 t
ORIGIN
1 ggcagcactc aggacaatct ccagcatggc ctggtctcct ctcctcctca ctctcctcgc
61 tcactgcaca gggtcctggg cccagtctgt tctggcgcag ccgccctcag tgtctggggc
121 cccagggcag acggtcacca tctcgtgcac tgggagcagc accaacatcg gggcgggtta
181 tgctgtacac tggtaccagc agtttccagg agcggccccc aaagtcctca tctatggtaa
241 ctacaatcgg ccctcagggg tccctgaccg attctctggc tccaagtctg gcacctcagc
301 ctccctggcc atcactgggc tccaggctga agatgaggct gattattact gccagtcgta
361 tgacggcagc ctgagtggtt ctgtcttcgg agctgggacc aaggtcaccg tcctgggtca
421 gcccaaggcc aaccccactg tcactctgtt cccgccctcc tctgaggagc tccaagccaa
481 caaggccaca ctagtgtgtc tgatcagtga cttctacccg ggagctgtga cagtggcctg
541 gaaggcagat ggcagccccg tcaaggcggg agtggagacc accaaaccct ccaaacagag
601 caacaacaag tacgcggcca gcagctacct gagcctgacg cccgagcagt ggaagtccca
661 cagaagctac agctgccagg tcacgcatga agggagcacc gtggagaaga cagtggcccc
721 tacagaatgt tcataggttc ccaactctaa ccccacccac gggagcctgg agctgcagga
781 tcccagggga ggggtctctc tccccatccc aagtcatcca gcccttctcc ctgcactcat
841 gaaaccccaa taaatatcct cattgacaac cagaaaaaaa aaaaaaaaaa aaaaaaaaa
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