LOCUS BC012854 1729 bp mRNA linear HUM 04-JUN-2004
DEFINITION Homo sapiens actin, beta, mRNA (cDNA clone IMAGE:3863361), partial
cds.
ACCESSION BC012854
VERSION BC012854.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1729)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1729)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 21 Row: h Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1729
/db_xref="H-InvDB:HIT000035998"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3863361"
/tissue_type="Ovary, adenocarcinoma"
/clone_lib="NIH_MGC_66"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..1729
/gene="ACTB"
/db_xref="GeneID:60"
/db_xref="MIM:102630"
CDS <1..1085
/gene="ACTB"
/codon_start=3
/product="ACTB protein"
/protein_id="AAH12854.1"
/db_xref="GeneID:60"
/db_xref="MIM:102630"
/translation="MCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ
SKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANLE
KMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAI
LRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASS
SSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRK
DLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASL
STFQQMWISKQEYDESGPSIVHRKCF"
BASE COUNT 414 a 485 c 424 g 406 t
ORIGIN
1 gcatgtgcaa ggccggcttc gcgggcgacg atgccccccg ggccgtcttc ccctccatcg
61 tggggcgccc caggcaccag ggcgtgatgg tgggcatggg tcagaaggat tcctatgtgg
121 gcgacgaggc ccagagcaag agaggcatcc tcaccctgaa gtaccccatc gagcacggca
181 tcgtcaccaa ctgggacgac atggagaaaa tctggcacca caccttctac aatgagctgc
241 gtgtggctcc cgaggagcac cccgtgctgc tgaccgaggc ccccctgaac cccaaggcca
301 acctcgagaa gatgacccag atcatgtttg agaccttcaa caccccagcc atgtacgttg
361 ctatccaggc tgtgctatcc ctgtacgcct ctggccgtac cactggcatc gtgatggact
421 ccggtgacgg ggtcacccac actgtgccca tctacgaggg gtatgccctc ccccatgcca
481 tcctgcgtct ggacctggct ggccgggacc tgactgacta cctcatgaag atcctcaccg
541 agcgcggcta cagcttcacc accacggccg agcgggaaat cgtgcgtgac attaaggaga
601 agctgtgcta cgtcgccctg gacttcgagc aagagatggc cacggctgct tccagctcct
661 ccctggagaa gagctacgag ctgcctgacg gccaggtcat caccattggc aatgagcggt
721 tccgctgccc tgaggcactc ttccagcctt ccttcctggg catggagtcc tgtggcatcc
781 acgaaactac cttcaactcc atcatgaagt gtgacgtgga catccgcaaa gacctgtacg
841 ccaacacagt gctgtctggc ggcaccacca tgtaccctgg cattgccgac aggatgcaga
901 aggagatcac tgccctggca cccagcacaa tgaagatcaa gatcattgct cctcctgagc
961 gcaagtactc cgtgtggatc ggcggctcca tcctggcctc gctgtccacc ttccagcaga
1021 tgtggatcag caagcaggag tatgacgagt ccggcccctc catcgtccac cgcaaatgct
1081 tctaggcgga ctatgactta gttgcgttac accctttctt gacaaaacct aacttgcgca
1141 gaaaacaaga tgagattggc atggctttat ttgttttttt tgttttgttt tggttttttt
1201 tttttttggc ttgactcagg atttaaaaac tggaacggtg aaggtgacag cagtcggttg
1261 gagcgagcat cccccaaagt tcacaatgtg gccgaggact ttgattgcac attgttgttt
1321 ttttaatagt cattccaaat atgagatgcg ttgttacagg aagtcccttg ccatcctaaa
1381 agccacccca cttctctcta aggagaatgg cccagtcctc tcccaagtcc acacagggga
1441 ggtgatagca ttgctttcgt gtaaattatg taatgcaaaa tttttttaat cttcgcctta
1501 atactttttt attttgtttt attttgaatg atgagccttc gtgccccccc ttcccccttt
1561 tttttgtccc ccaacttgag atgtatgaag gcttttggtc tccctgggag tgggtggagg
1621 cagccagggc ttacctgtac actgacttga gaccagttga ataaaagtgc acaccttaaa
1681 aatgaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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