LOCUS BC012811 2336 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:3932
IMAGE:2958817), complete cds.
ACCESSION BC012811
VERSION BC012811.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2336)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2336)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC012811.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 10 Row: e Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33286421.
FEATURES Location/Qualifiers
source 1..2336
/db_xref="H-InvDB:HIT000035967"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3932 IMAGE:2958817"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2336
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
CDS 101..1696
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/codon_start=1
/product="pyruvate kinase, muscle"
/protein_id="AAH12811.3"
/db_xref="GeneID:5315"
/db_xref="HGNC:HGNC:9021"
/db_xref="MIM:179050"
/translation="MSKPHSQAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN
TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDP
ILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLD
YKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDL
PAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEG
VRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESM
IKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL
QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPI
IAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGHV
VIVLTGWRPGSGFTNTMRVVPVP"
BASE COUNT 535 a 656 c 654 g 491 t
ORIGIN
1 cagtggctcc ttgcacagca gctgcacgcg ccgtggctcc ggatctcttc gtctttgcag
61 cgtagcccga gtcggtcagc gccggaggac ctcagcagcc atgtcgaagc cccatagtca
121 agccgggact gccttcattc agacccagca gctgcacgca gccatggctg acacattcct
181 ggagcacatg tgccgcctgg acattgattc accacccatc acagcccgga acactggcat
241 catctgtacc attggcccag cttcccgatc agtggagacg ttgaaggaga tgattaagtc
301 tggaatgaat gtggctcgtc tgaacttctc tcatggaact catgagtacc atgcggagac
361 catcaagaat gtgcgcacag ccacggaaag ctttgcttct gaccccatcc tctaccggcc
421 cgttgctgtg gctctagaca ctaaaggacc tgagatccga actgggctca tcaagggcag
481 cggcactgca gaggtggagc tgaagaaggg agccactctc aaaatcacgc tggataacgc
541 ctacatggaa aagtgtgacg agaacatcct gtggctggac tacaagaaca tctgcaaggt
601 ggtggaagtg ggcagcaaga tctacgtgga tgatgggctt atttctctcc aggtgaagca
661 gaaaggtgcc gacttcctgg tgacggaggt ggaaaatggt ggctccttgg gcagcaagaa
721 gggtgtgaac cttcctgggg ctgctgtgga cttgcctgct gtgtcggaga aggacatcca
781 ggatctgaag tttggggtcg agcaggatgt tgatatggtg tttgcgtcat tcatccgcaa
841 ggcatctgat gtccatgaag ttaggaaggt cctgggagag aagggaaaga acatcaagat
901 tatcagcaaa atcgagaatc atgagggggt tcggaggttt gatgaaatcc tggaggccag
961 tgatgggatc atggtggctc gtggtgatct aggcattgag attcctgcag agaaggtctt
1021 ccttgctcag aagatgatga ttggacggtg caaccgagct gggaagcctg tcatctgtgc
1081 tactcagatg ctggagagca tgatcaagaa gccccgcccc actcgggctg aaggcagtga
1141 tgtggccaat gcagtcctgg atggagccga ctgcatcatg ctgtctggag aaacagccaa
1201 aggggactat cctctggagg ctgtgcgcat gcagcacctg attgcccgtg aggcagaggc
1261 tgccatctac cacttgcaat tatttgagga actccgccgc ctggcgccca ttaccagcga
1321 ccccacagaa gccaccgccg tgggtgccgt ggaggcctcc ttcaagtgct gcagtggggc
1381 cataatcgtc ctcaccaagt ctggcaggtc tgctcaccag gtggccagat accgcccacg
1441 tgcccccatc attgctgtga cccggaatcc ccagacagct cgtcaggccc acctgtaccg
1501 tggcatcttc cctgtgctgt gcaaggaccc agtccaggag gcctgggctg aggacgtgga
1561 cctccgggtg aactttgcca tgaatgttgg caaggcccga ggcttcttca agaagggaca
1621 tgtggtcatt gtgctgaccg gatggcgccc tggctccggc ttcaccaaca ccatgcgtgt
1681 tgttcctgtg ccgtgatgga ccccagagcc cctcctccag cccctgtccc acccccttcc
1741 cccagcccat ccattaggcc agcaacgctt gtagaactca ctctgggctg taacgtggca
1801 ctggtaggtt gggacaccag ggaagaagat caacgcctca ctgaaacatg gctgtgtttg
1861 cagcctgctc tagtgggaca gcccagagcc tggctgccca tcatgtggcc ccacccaatc
1921 aagggaagaa ggaggaatgc tggactggag gcccctggag ccagatggca agagggtgac
1981 agcttccttt cctgtgtgta ctctgtccag ttcctttaga aaaaatggat gcccagagga
2041 ctcccaaccc tggcttgggg tcaagaaaca gccagcaaga gttaggggcc ttagggcact
2101 gggctgttgt tccattgaag ccgactctgg ccctggccct tacttgcttc tctagctctc
2161 taggcctctc cagtttgcac ctgtccccac cctccactca gctgtcctgc agcaaacact
2221 ccaccctcca ccttccattt tcccccacta ctgcagcacc tccaggcctg ttgctataga
2281 gcctacctgt atgtcaataa acaacagctg aagcaaaaaa aaaaaaaaaa aaaaaa
//