LOCUS BC012808 4078 bp mRNA linear HUM 19-JUN-2006
DEFINITION Homo sapiens valyl-tRNA synthetase, mRNA (cDNA clone MGC:3537
IMAGE:3529215), complete cds.
ACCESSION BC012808
VERSION BC012808.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4078)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4078)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC012808.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: a Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5454157.
FEATURES Location/Qualifiers
source 1..4078
/db_xref="H-InvDB:HIT000035964_04"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3537 IMAGE:3529215"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..4078
/gene="VARS"
/gene_synonym="G7A"
/db_xref="GeneID:7407"
/db_xref="HGNC:HGNC:12651"
/db_xref="MIM:192150"
CDS 194..3988
/gene="VARS"
/gene_synonym="G7A"
/codon_start=1
/product="valyl-tRNA synthetase"
/protein_id="AAH12808.1"
/db_xref="GeneID:7407"
/db_xref="HGNC:HGNC:12651"
/db_xref="MIM:192150"
/translation="MSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQP
PPTSRTSFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSY
ADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALSPLEEWLRLHTYLAGEAPTLA
DLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLYSGARPLSH
QPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPG
VITYDLPTPPGEKKDVSGPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPR
GVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVV
EKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWDRACFTMDPK
LSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKE
KVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDTRYQHLKGKNVIH
PFLSRSLPIVFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINV
PPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRC
GEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSD
PAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPL
SILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVR
DAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGI
PECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSP
TSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECL
KPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCV
TPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGAL
ASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKL
QAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQK
ML"
BASE COUNT 797 a 1260 c 1243 g 778 t
ORIGIN
1 ggctgccggg gcggcgatct tagggaacta gggtcacctg gagagccgcc caccgtctct
61 gcccgctcga ctcctccgcc cgggccgctc ggccggtcca gccgcggccg gcgcctggct
121 gtgaggtgga ttcccggccc agtctgacca tctccctcca gtttttccac ttcgttcgga
181 ccttctcata actatgtcca ccctctacgt ctcccctcac ccagatgcct tccccagcct
241 ccgagccctc atagccgctc gctatgggga ggctggggag ggtcccggat ggggaggagc
301 ccacccccgc atctgtctcc agccaccccc gactagcagg actagctttc ccccaccccg
361 cctgccggcc ctggagcagg ggcccggtgg gctctgggtg tggggggcca cggctgtggc
421 ccagctgctg tggccagcag gcctgggggg cccagggggc agccgggcgg ctgtccttgt
481 ccaacagtgg gtcagttacg ccgacacgga gttaatacca gctgcctgtg gagcaacgct
541 gccggccctg ggactccgaa gctcggccca ggacccccag gctgtgctgg gggccctggg
601 cagggccctg agccccttgg aggagtggct tcggctgcac acctacttgg ccggggaggc
661 ccccactctg gctgacctgg cggctgtcac agccttgctg ctgcctttcc gatacgtcct
721 agacccacct gcccgccgga tctggaataa tgtgactcgc tggtttgtca cgtgtgtccg
781 gcagccagaa ttccgagccg tgctaggaga agtggttcta tactcaggag ccaggcctct
841 ctctcatcag ccaggccccg aggctcctgc cctcccaaag acagctgctc agctcaagaa
901 agaggcaaag aaacgggaga agctagagaa attccaacag aagcagaaga tccaacagca
961 gcagccacct ccaggggaga agaaaccaaa accagagaag agggagaaac gggatcctgg
1021 ggtcattacc tatgacctcc caaccccacc cggggaaaag aaagatgtca gtggccccat
1081 gcccgactcc tacagccctc ggtatgtgga ggctgcctgg tacccttggt gggagcagca
1141 gggcttcttc aagccagagt atgggcgtcc taatgtgtca gcagcaaatc cccgaggtgt
1201 cttcatgatg tgcatcccac cccccaatgt gacaggctcc ctgcacctgg gccatgcact
1261 caccaacgcc atccaggact ccctgactcg atggcaccgc atgcgtgggg agaccaccct
1321 gtggaaccct ggctgtgacc atgcaggtat tgccacccag gtggtggtgg agaagaagct
1381 atggcgtgag cagggactga gccggcacca gctgggccgc gaggcctttc tacaggaagt
1441 ctggaagtgg aaggaggaga aaggtgaccg gatttaccac cagttgaaga agcttggcag
1501 ctccttggac tgggatcgag cctgttttac catggaccct aaactctcag cagctgtgac
1561 agaggccttt gtccggcttc acgaggaagg catcatctat cgcagtaccc gccttgttaa
1621 ctggtcctgc accctcaact ccgccatctc tgacattgag gtggataaga aggagctgac
1681 aggtcgcacc ctgctctccg tgcctggcta caaggagaag gtggagttcg gggtcctcgt
1741 gtcctttgcc tataaggtcc aaggctcaga tagcgacgag gaggtggtgg tggcaacaac
1801 tcggatcgag acaatgctgg gagatgtggc tgtagctgtg caccccaaag ataccagata
1861 ccagcacctg aaggggaaga acgtgatcca cccattcctg tctcggagcc ttcccattgt
1921 cttcgatgaa tttgtggaca tggactttgg cacaggtgct gtgaagatca cccccgcaca
1981 tgaccaaaat gactatgaag ttgggcagcg gcacgggctg gaggccatca gcatcatgga
2041 ctcccggggg gccctcatca atgtgcctcc gcctttcctg ggcctgccca ggtttgaggc
2101 caggaaagcg gtgctggtgg cgctgaagga gcggggactg ttccgtggca ttgaggacaa
2161 ccccatggtg gtgccacttt gcaaccggtc gaaggacgtg gtagagcctc tgctgcggcc
2221 gcagtggtac gttcgctgcg gggagatggc ccaggctgcc agcgccgctg tgactcgggg
2281 tgacctccgc atcctgcctg aggcccatca gcgcacatgg catgcctgga tggacaacat
2341 ccgggagtgg tgcatttcca ggcagctgtg gtggggccat cgcatcccag cctactttgt
2401 cactgtcagt gacccagcgg tgccccctgg ggaggaccct gatgggcggt actgggtgag
2461 tggacgcaat gaggcggagg cccgggagaa ggcagccaag gagttcggag tgtcccctga
2521 caagatcagt ctccagcaag atgaggatgt attggatacc tggttctcct ctggcctctt
2581 ccccttatcc attttgggct ggcccaacca gtcagaagac ctgagtgtgt tctaccccgg
2641 gacactgctg gagaccggtc atgacatcct cttcttctgg gtggcccgga tggtcatgct
2701 gggcctgaag ctcacgggca ggctgccctt tagagaggtc tacctccatg ccatcgtgcg
2761 agatgctcac ggccggaaga tgagcaagtc tctaggcaat gtcatcgatc ccctggacgt
2821 catctatgga atctccctgc agggcctcca caaccagctg ctgaacagca acctggatcc
2881 cagcgaggtg gagaaggcca aagaagggca gaaagctgac ttcccagcgg ggattcctga
2941 atgtggcacc gatgctctcc ggtttggatt atgtgcctac atgtcccagg gtcgtgacat
3001 caacctggat gtgaaccgga tactgggtta ccgccacttc tgcaacaagc tctggaatgc
3061 caccaagttt gcccttcgtg gccttgggaa gggttttgtg ccctcaccca cctcccagcc
3121 cggaggccat gagagcctgg tggaccgctg gatccgcagc cgcctgacag aggctgtgag
3181 gctcagcaat caaggcttcc aggcctacga cttcccggcc gtcaccactg cccagtacag
3241 cttctggctc tatgagctct gtgatgtcta cttggagtgc ctgaaacctg tactgaatgg
3301 ggtggaccag gtggcagctg agtgtgcccg ccagaccctg tacacttgcc tggacgttgg
3361 cctgcggctg ctctcaccct tcatgccctt cgtgacggag gagctgttcc agaggctgcc
3421 ccggaggatg ccgcaagctc cccctagcct ctgtgttacc ccctacccgg agccctcaga
3481 gtgctcctgg aaggaccccg aggcagaagc cgcccttgag ctggcgctaa gcatcacgcg
3541 agccgtgcgc tccctgcggg ccgactacaa cctcacccgg atccggcctg actgtttcct
3601 ggaagtggcg gatgaggcca cgggcgccct ggcatcggcg gtgtcgggct acgtgcaggc
3661 cctggccagc gcaggtgtgg tggctgttct ggccctgggg gctcccgccc cccagggttg
3721 cgctgtggct ctggcttctg atcgctgctc catccacctg cagcttcagg ggctggtgga
3781 ccctgcacgg gagctgggca agctgcaagc caagcgagtt gaggcccagc ggcaggccca
3841 gcgtctgcgg gaacgccgtg ctgcctcggg ctatcctgtc aaggtgccgc tcgaagtcca
3901 ggaggcagat gaagccaagc tccaacagac agaagcagag ctcaggaagg tggatgaggc
3961 catcgcccta ttccagaaga tgctgtgatc caccacccag cttcacccct cacccccagc
4021 ggctcaccat ggggatggca gcaataaaat attttcccac aaaaaaaaaa aaaaaaaa
//