LOCUS BC012732 2045 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens nonhomologous end-joining factor 1, mRNA (cDNA clone
MGC:15029 IMAGE:4026495), complete cds.
ACCESSION BC012732
VERSION BC012732.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2045)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2045)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC012732.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 25 Row: n Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13376141.
FEATURES Location/Qualifiers
source 1..2045
/db_xref="H-InvDB:HIT000035920"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15029 IMAGE:4026495"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2045
/gene="NHEJ1"
/gene_synonym="FLJ12610"
/gene_synonym="XLF"
/db_xref="GeneID:79840"
/db_xref="HGNC:HGNC:25737"
CDS 68..967
/gene="NHEJ1"
/gene_synonym="FLJ12610"
/gene_synonym="XLF"
/codon_start=1
/product="nonhomologous end-joining factor 1"
/protein_id="AAH12732.1"
/db_xref="GeneID:79840"
/db_xref="HGNC:HGNC:25737"
/translation="MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQV
WHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVAD
ALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMK
DLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLY
MAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTG
TSGPLQRPQLSKVKRKKPRGLFS"
BASE COUNT 527 a 504 c 475 g 539 t
ORIGIN
1 gtttgcgcga gcgggcagga aagcgtgcgt gcggctaaga gagtgggcgc tctcgcggcc
61 gctgacgatg gaagaactgg agcaaggcct gttgatgcag ccatgggcgt ggctacagct
121 tgcagagaac tccctcttgg ccaaggtttt tatcaccaag cagggctatg ccttgttggt
181 ttcagatctt caacaggtgt ggcatgaaca ggtggacact agtgtggtca gccagcgagc
241 caaggagctg aacaagcggc tcactgctcc tcctgcagct ttcctctgtc atttggataa
301 tctccttcgc ccattgttga aggacgctgc tcaccctagc gaagctacct tctcctgtga
361 ttgtgtggca gatgcactga ttctacgggt gcgaagtgag ctctctggcc tccccttcta
421 ttggaatttc cactgcatgc tagctagtcc ttccctggtc tcccaacatt tgattcgtcc
481 tctgatgggc atgagtctgg cattacagtg ccaagtgagg gagctagcaa cgttacttca
541 tatgaaagac ctagagatcc aagactacca ggagagtggg gctacgctga ttcgagatcg
601 attgaagaca gaaccatttg aagaaaattc cttcttggaa caatttatga tagagaaact
661 gccagaggca tgcagcattg gtgatggaaa gccctttgtc atgaatctgc aggatctgta
721 tatggcagtc accacacaag aggtccaagt gggacagaag catcaaggcg ctggagatcc
781 tcatacctca aacagtgctt ccctgcaagg aatcgatagc caatgtgtaa accagccaga
841 acaactggtc tcctcagccc caaccctctc agcacctgag aaagagtcca cgggtacttc
901 aggccctctg cagagacctc agctgtcaaa ggtcaagagg aagaagccaa ggggtctctt
961 cagttaatct gttgtggcct cagctgctga ggatggactt ggagaatagc ttccaagctt
1021 caccttgaaa gaagcttaca tggcagcaat atttctaaaa tagtgataca gtcagaggcc
1081 tcctgtaagg gcgagagaac tgaagttgat gttgacaggc ccacagggaa ttggccttcc
1141 ctgttcaagt ggaagccagt ctctgagaat cccgtgctct cctctctttt ggtggaggtt
1201 ctgtaggttc aggtttctac catggacttt aggtatatag ggcaagtcag caagaaagca
1261 ccacacactc aggaagcctt gtctaccttt ccctagcgtc tctagccagc cagccccaga
1321 tactcctcag agacccactt ctctcttttg catggaataa aaagcactca cagtccctgc
1381 ttttgggatt acttatgctg tggaactcat aacccaattc acttccttca ctgggtccca
1441 ccccattgtt gtctctggat gaagtctagc catcagtctg gtccttcaga ttcttcgtaa
1501 acttttctgc agttgccaga gctaagtctc catttggaaa ctttgatctc aagaactctc
1561 ttgatggtgg gcacaagaca gatagatgat tgctgctttc ctttgccaaa cttggatgca
1621 tttttctctt tgtttccaaa gtataggcaa caggttaaac taccacgtgg ggtagtttat
1681 caatcctccc gcctcatcct tctgcataac tgggactaaa ggcacacacc accacaccta
1741 gctaattttt gtattttttt gtgtgtggag acaaggcttt gccatgttgc ccaggctggt
1801 cttgaactcc tggactctag tgatctgccc acctcaacct cccaaagtgc tgggattaca
1861 ggcatgagct accacgccta tccaatttct ggtttattta ctttcgatgg ttaaatgcct
1921 ccttattcaa atatcctgta tatatactgg cgtttctgtc ctgatgtcaa gtctcgagag
1981 aagatgatca aacaataata aaataattat aataagaaca acaataaaaa aaaaaaaaaa
2041 aaaaa
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