LOCUS BC012089 3983 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens prominin 1, mRNA (cDNA clone MGC:20041 IMAGE:4644690),
complete cds.
ACCESSION BC012089
VERSION BC012089.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3983)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3983)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 29 Row: m Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5174386.
FEATURES Location/Qualifiers
source 1..3983
/db_xref="H-InvDB:HIT000035649"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20041 IMAGE:4644690"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3983
/gene="PROM1"
/gene_synonym="AC133"
/gene_synonym="CD133"
/gene_synonym="PROML1"
/db_xref="GeneID:8842"
/db_xref="MIM:604365"
CDS 236..2806
/gene="PROM1"
/gene_synonym="AC133"
/gene_synonym="CD133"
/gene_synonym="PROML1"
/codon_start=1
/product="PROM1 protein"
/protein_id="AAH12089.1"
/db_xref="GeneID:8842"
/db_xref="MIM:604365"
/translation="MALVLGSLLLLGLCGNSFSGGQPSSTDAPKAWNYELPATNYETQ
DSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKIDYDKIVYYEAGIIL
CCVLGLLFIILMPLVGYFFCMCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIII
SIGIFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAF
TDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQST
QLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELD
NVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLP
IQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCG
VCGYDRHATPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICE
PYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHL
QNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDS
YLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQS
LSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGY
FEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLP
ALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDHVYGIHNPVMTSPSQH
"
BASE COUNT 1168 a 828 c 854 g 1133 t
ORIGIN
1 atgctctcag ctctcccgcc gcgggatggt gccttgagtg aatgaccccc ttggagaaca
61 ttcttccgca tccctcgcct caagccagcc tcagacagaa aactgaagat tcagcagatc
121 cagtgcttcc tgctcctctt ctgcccagga acacgcttgc cttccccaag gcttccagaa
181 gctctgaggc aggaggcacc aagttctacc tcatgtttgg aggatcttgc tagctatggc
241 cctcgtactc ggctccctgt tgctgctggg gctgtgcggg aactcctttt caggagggca
301 gccttcatcc acagatgctc ctaaggcttg gaattatgaa ttgcctgcaa caaattatga
361 gacccaagac tcccataaag ctggacccat tggcattctc tttgaactag tgcatatctt
421 tctctatgtg gtacagccgc gtgatttccc agaagatact ttgagaaaat tcttacagaa
481 ggcatatgaa tccaaaattg attatgacaa gattgtctac tatgaagcag ggattattct
541 atgctgtgtc ctggggctgc tgtttattat tctgatgcct ctggtggggt atttcttttg
601 tatgtgtcgt tgctgtaaca aatgtggtgg agaaatgcac cagcgacaga aggaaaatgg
661 gcccttcctg aggaaatgct ttgcaatctc cctgttggtg atttgtataa taataagcat
721 tggcatcttc tatggttttg tggcaaatca ccaggtaaga acccggatca aaaggagtcg
781 gaaactggca gatagcaatt tcaaggactt gcgaactctc ttgaatgaaa ctccagagca
841 aatcaaatat atattggccc agtacaacac taccaaggac aaggcgttca cagatctgaa
901 cagtatcaat tcagtgctag gaggcggaat tcttgaccga ctgagaccca acatcatccc
961 tgttcttgat gagattaagt ccatggcaac agcgatcaag gagaccaaag aggcgttgga
1021 gaacatgaac agcaccttga agagcttgca ccaacaaagt acacagctta gcagcagtct
1081 gaccagcgtg aaaactagcc tgcggtcatc tctcaatgac cctctgtgct tggtgcatcc
1141 atcaagtgaa acctgcaaca gcatcagatt gtctctaagc cagctgaata gcaaccctga
1201 actgaggcag cttccacccg tggatgcaga acttgacaac gttaataacg ttcttaggac
1261 agatttggat ggcctggtcc aacagggcta tcaatccctt aatgatatac ctgacagagt
1321 acaacgccaa accacgactg tcgtagcagg tatcaaaagg gtcttgaatt ccattggttc
1381 agatatcgac aatgtaactc agcgtcttcc tattcaggat atactctcag cattctctgt
1441 ttatgttaat aacactgaaa gttacatcca cagaaattta cctacattgg aagagtatga
1501 ttcatactgg tggctgggtg gcctggtcat ctgctctctg ctgaccctca tcgtgatttt
1561 ttactacctg ggcttactgt gtggcgtgtg cggctatgac aggcatgcca ccccgaccac
1621 ccgaggctgt gtctccaaca ccggaggcgt cttcctcatg gttggagttg gattaagttt
1681 cctcttttgc tggatattga tgatcattgt ggttcttacc tttgtctttg gtgcaaatgt
1741 ggaaaaactg atctgtgaac cttacacgag caaggaatta ttccgggttt tggatacacc
1801 ctacttacta aatgaagact gggaatacta tctctctggg aagctattta ataaatcaaa
1861 aatgaagctc acttttgaac aagtttacag tgactgcaaa aaaaatagag gcacttacgg
1921 cactcttcac ctgcagaaca gcttcaatat cagtgaacat ctcaacatta atgagcatac
1981 tggaagcata agcagtgaat tggaaagtct gaaggtaaat cttaatatct ttctgttggg
2041 tgcagcagga agaaaaaacc ttcaggattt tgctgcttgt ggaatagaca gaatgaatta
2101 tgacagctac ttggctcaga ctggtaaatc ccccgcagga gtgaatcttt tatcatttgc
2161 atatgatcta gaagcaaaag caaacagttt gcccccagga aatttgagga actccctgaa
2221 aagagatgca caaactatta aaacaattca ccagcaacga gtccttccta tagaacaatc
2281 actgagcact ctataccaaa gcgtcaagat acttcaacgc acagggaatg gattgttgga
2341 gagagtaact aggattctag cttctctgga ttttgctcag aacttcatca caaacaatac
2401 ttcctctgtt attattgagg aaactaagaa gtatgggaga acaataatag gatattttga
2461 acattatctg cagtggatcg agttctctat cagtgagaaa gtggcatcgt gcaaacctgt
2521 ggccaccgct ctagatactg ctgttgatgt ctttctgtgt agctacatta tcgacccctt
2581 gaatttgttt tggtttggca taggaaaagc tactgtattt ttacttccgg ctctaatttt
2641 tgcggtaaaa ctggctaagt actatcgtcg aatggattcg gaggacgtgt acgatgatgt
2701 tgaaactata cccatgaaaa atatggaaaa tggtaataat ggttatcata aagatcatgt
2761 atatggtatt cacaatcctg ttatgacaag cccatcacaa cattgatagc tgatgttgaa
2821 actgcttgag catcaggata ctcaaagtgg aaaggatcac agatttttgg tagtttctgg
2881 gtctacaagg actttccaaa tccaggagca acgccagtgg caacgtagtg actcaggcgg
2941 gcaccaaggc aacggcacca ttggtctctg ggtagtgctt taagaatgaa cacaatcacg
3001 ttatagtcca tggtccatca ctattcaagg atgactccct cccttcctgt ctatttttgt
3061 tttttacttt tttacactga gtttctattt agacactaca acatatgggg tgtttgttcc
3121 cattggatgc atttctatca aaactctatc aaatgtgatg gctagattct aacatattgc
3181 catgtgtgga gtgtgctgaa cacacaccag tttacaggaa agatgcattt tgtgtacagt
3241 aaacggtgta tatacctttt gttaccacag agttttttaa acaaatgagt attataggac
3301 tttcttctaa atgagctaaa taagtcacca ttgacttctt ggtgctgttg aaaataatcc
3361 attttcacta aaagtgtgtg aaacctacag catattcttc acgcagagat tttcatctat
3421 tatactttat caaagattgg ccatgttcca cttggaaatg gcatgcaaaa gccatcatag
3481 agaaacctgc gtaactccat ctgacaaatt caaaagagag agagagatct tgagagagaa
3541 atgctgttcg ttcaaaagtg gagttgtttt aacagatgcc aattacggtg tacagtttaa
3601 cagagttttc tgttgcatta ggataaacat taattggagt gcagctaaca tgagtatcat
3661 cagactagta tcaagtgttc taaaatgaaa tatgagaaga tcctgtcaca attcttagat
3721 ctggtgtcca gcatggatga aacctttgag tttggtccct aaatttgcat gaaagcacaa
3781 ggtaaatatt catttgcttc aggagtttca tgttggatct gtcattatca aaagtgatca
3841 gcaatgaaga actggtcgga caaaatttaa cgttgatgta atggaattcc agatgtaggc
3901 attcccccca ggtcttttca tgtgcagatt gcagttctga ttcatttgaa taaaaaggaa
3961 cttggaaaaa aaaaaaaaaa aaa
//