LOCUS BC011927 2560 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA
clone IMAGE:4544412), partial cds.
ACCESSION BC011927
VERSION BC011927.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2560)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2560)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC011927.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: o Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: Similarity but not
identity to protein.
FEATURES Location/Qualifiers
source 1..2560
/db_xref="H-InvDB:HIT000035546"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4544412"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2560
/gene="DDX27"
/gene_synonym="dJ686N3.1"
/gene_synonym="DKFZp667N057"
/gene_synonym="FLJ12917"
/gene_synonym="FLJ20596"
/gene_synonym="FLJ22238"
/gene_synonym="HSPC259"
/gene_synonym="MGC1018"
/gene_synonym="PP3241"
/gene_synonym="RHLP"
/gene_synonym="Rrp3p"
/db_xref="GeneID:55661"
CDS <1..2306
/gene="DDX27"
/gene_synonym="dJ686N3.1"
/gene_synonym="DKFZp667N057"
/gene_synonym="FLJ12917"
/gene_synonym="FLJ20596"
/gene_synonym="FLJ22238"
/gene_synonym="HSPC259"
/gene_synonym="MGC1018"
/gene_synonym="PP3241"
/gene_synonym="RHLP"
/gene_synonym="Rrp3p"
/codon_start=3
/product="DDX27 protein"
/protein_id="AAH11927.2"
/db_xref="GeneID:55661"
/translation="DNMLADLGLIGTIGEDDEVPVEPESDSGDEEEEGPIVLGRRQKA
LGKNRSADFNPDFVFTEKEGTYDGSWALADVMSQLKKKRAATTLDEKIEKVRKKRKTE
DKEAKSGKLEKEKEAKEGSEPKEQEDLQENDEEGSEDEASETDYSSADENILTKADTL
KVKDRKKKKKKGQEAGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKA
CIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAPVTRVLVLVPTRELGIQV
HSVTRQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLS
SIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPV
RIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRM
HILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVIN
FTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDV
ILKFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQINTAKRLLEKGKEAVVQEPERSWF
QTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFA
ERLAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRASPSF
EERKQLGLPHQRRGGNFKSKSRYKRRK"
misc_feature 573..1187
/gene="DDX27"
/gene_synonym="dJ686N3.1"
/gene_synonym="DKFZp667N057"
/gene_synonym="FLJ12917"
/gene_synonym="FLJ20596"
/gene_synonym="FLJ22238"
/gene_synonym="HSPC259"
/gene_synonym="MGC1018"
/gene_synonym="PP3241"
/gene_synonym="RHLP"
/gene_synonym="Rrp3p"
/note="DEADc; Region: DEAD-box helicases. A diverse family
of proteins involved in ATP-dependent RNA unwinding,
needed in a variety of cellular processes including
splicing, ribosome biogenesis and RNA degradation. The
name derives from the sequence of the Walker B motif
(motif II). This domain contains the ATP- binding region"
/db_xref="CDD:cd00268"
misc_feature 1230..1562
/gene="DDX27"
/gene_synonym="dJ686N3.1"
/gene_synonym="DKFZp667N057"
/gene_synonym="FLJ12917"
/gene_synonym="FLJ20596"
/gene_synonym="FLJ22238"
/gene_synonym="HSPC259"
/gene_synonym="MGC1018"
/gene_synonym="PP3241"
/gene_synonym="RHLP"
/gene_synonym="Rrp3p"
/note="HELICc; Region: Helicase superfamily c-terminal
domain"
/db_xref="CDD:cd00079"
BASE COUNT 748 a 591 c 726 g 495 t
ORIGIN
1 gcgacaacat gcttgcggac ctcggcttaa tcggaaccat aggcgaggat gacgaggtgc
61 cggtggagcc cgagtctgac tccggggacg aggaagagga ggggcccatt gtgctgggca
121 gacgacaaaa agctttgggg aagaaccgca gtgctgattt caaccctgat ttcgttttca
181 ctgagaagga ggggacgtac gatggcagct gggccctggc tgatgtcatg agccaactca
241 agaagaagag ggcagccact acattagatg agaagattga gaaagttcga aagaaaagga
301 aaacagagga taaagaagcc aagtctggga agttggaaaa ggagaaagaa gcaaaggaag
361 gctctgaacc aaaggagcag gaagaccttc aagagaatga tgaggaaggc tcagaagatg
421 aagcctcgga gactgactac tcatcagctg atgagaacat cctcaccaaa gcagatacac
481 tcaaagtaaa ggatcggaag aagaagaaga agaaaggaca ggaagcagga ggattttttg
541 aagatgcatc tcagtacgat gaaaacctct cgttccagga catgaacctt tcccgccctc
601 ttctgaaggc cattacagcc atgggcttca agcagcccac cccgatccag aaggcgtgca
661 tacctgtggg tctattgggg aaggacatct gtgcctgtgc agccactggg acaggtaaaa
721 ctgccgcctt tgccctgcct gttttggagc gtctgattta taaaccccgc caggctccag
781 tcacccgcgt gctggtgcta gtgcccaccc gagagctggg catccaggtg cactctgtca
841 ccagacagct ggcccagttc tgcaacatca ccacctgcct ggctgtgggc ggcttggatg
901 tgaagtctca ggaagcagct cttcgggcag cgcctgacat cctcatcgcc accccaggcc
961 ggctcatcga tcacctccac aactgccctt ccttccacct gagcagcatc gaggtgctca
1021 tcctggacga ggctgacagg atgctggatg agtactttga ggagcagatg aaggagatca
1081 tccgaatgtg ttcccaccac cgccagacca tgctcttctc ggccaccatg acagacgagg
1141 tgaaagatct ggcttctgtc tccttgaaga atcctgtccg gatatttgtg aacagcaaca
1201 cagatgtggc tccctttctg cggcaggagt tcatccggat ccggcctaat cgtgaaggag
1261 accgggaagc catcgtggca gctttgttga cgaggacctt cactgaccat gtgatgctgt
1321 tcacgcaaac caagaagcag gcccaccgca tgcacatcct cctggggctc atggggctgc
1381 aggtgggtga gctccatggc aacttgtcac agacgcagcg gctggaggcc ctccggcgtt
1441 ttaaggatga acagattgac atcctcgtgg ccactgatgt ggcagcccgt ggacttgaca
1501 ttgagggggt caaaacggta atcaacttca caatgcctaa taccatcaaa cattatgtcc
1561 accgggtggg gcgaacagca cgtgctggca gggctgggcg ctcagtctct ctggtgggag
1621 aagatgagcg gaagatgctg aaggagattg taaaagctgc caaggcccct gtgaaggcca
1681 ggatacttcc ccaagatgtc atcctcaaat tccgggacaa gattgagaaa atggagaaag
1741 atgtgtatgc agttctgcag ctagaggcgg aggaaaaaga gatgcagcag tcagaagccc
1801 agatcaatac agcaaagcgg ctcctggaga aggggaagga ggcagtggtc caagagcccg
1861 agaggagctg gttccagacc aaagaagaga ggaagaagga gaaaattgcc aaagctctgc
1921 aggaatttga cttggcctta agaggaaaga agaaaaggaa gaagtttatg aaggatgcca
1981 aaaaaaaggg ggagatgaca gcagaggaaa ggtctcagtt tgaaatcctc aaggcgcaga
2041 tgtttgctga acggctagcg aagaggaatc gcagagccaa gcgggcccga gcaatgcccg
2101 aggaggagcc agtgagaggt cctgccaaga agcaaaagca ggggaagaaa tctgtatttg
2161 atgaagaact caccaacaca agcaagaagg ccctgaaaca gtatcgagct agcccttcct
2221 ttgaagaaag gaaacagttg ggcttgcccc accagagacg aggaggaaac tttaaatcta
2281 aatccagata caagaggagg aagtagctgt cgtggcctga agaaattcat gggggcagcc
2341 cttaaatccc ttccctgtgg gaagtcatcc tggctggtct gtcttttctc catttgttta
2401 aaaaaaaaac aaaaacaaaa aacaacactt tggtgtggtg gtatggtacg tagctatttt
2461 cctaagcatg tctgtcaatc tcccttcttg ctgattagct ttcatatgac tatattaaat
2521 ggaagtattt ttgggaaaag aaaaaaaaaa aaaaaaaaaa
//