LOCUS BC011891 2681 bp mRNA linear HUM 13-DEC-2004
DEFINITION Homo sapiens pleckstrin homology domain containing, family G (with
RhoGef domain) member 3, mRNA (cDNA clone IMAGE:3940399), partial
cds.
ACCESSION BC011891
VERSION BC011891.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2681)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2681)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC011891.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: g Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..2681
/db_xref="H-InvDB:HIT000035520"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3940399"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2681
/gene="PLEKHG3"
/db_xref="GeneID:26030"
CDS <1..2086
/gene="PLEKHG3"
/codon_start=2
/product="PLEKHG3 protein"
/protein_id="AAH11891.2"
/db_xref="GeneID:26030"
/translation="PSAEETPSDTESPEVLETQLDAHQGLLGMDPPGDMVDFVAAEST
EDLKALSSEEEEEMGGAAQEPESLLPPSVLDQASVIAERFVSSFSRRSSVAQEDSKSS
GFGSPRLVSRSSSVLSLEGSEKGLARHGSATDSLSCQLSPEVDISVGVATEDSPSVNG
MEPPSPGCPVEPDRSSCKKKESALSTRDRLLLDKIKSYYENAEHHDAGFSVRRRESLS
YIPKGLVRNSISRFNSLPRPDPEPVPPVGSKRQVGSRPTSWALFELPGPSQAVKGDPP
PISDAEFRPSSEIVKIWEGMESSGGSPGKGPGQGQANGFDLHEPLFILEEHELGAITE
ESATASPESSSPTEGRSPAHLARELKELVKELSSSTQGELVAPLHPRIVQLSHVMDSH
VSERVKNKVYQLARQYSLRIKSNKPVMARPPLQWEKVAPERDGKSPTVPCLQEEAGEP
LGGKGKRKPVLSLFDYEQLMAQEHSPPKPSSAGEMSPQRFFFNPSAVSQRTTSPGGRP
SARSPLSPTETFSWPDVRELCSKYASRDEARRAGGGRPRGPPVNRSHSVPENMVEPPL
SGRVGRCRSLSTKRGRGGGEAAQSPGPLPQSKPDGGETLYVTADLTLEDNRRVIVMEK
GPLPSPTAGLEESSGQGPSSPVALLGQVQDFQQSAECQPKEEGSRDPADPSQQGRVRN
LREKFQALNSVG"
BASE COUNT 600 a 792 c 836 g 453 t
ORIGIN
1 gcccagtgcc gaggagacgc cttcagacac agaatctcca gaagtcctgg agacacagct
61 tgatgcccac cagggccttc tggggatgga ccccccaggt gacatggtgg acttcgtggc
121 agctgagagc actgaggacc ttaaggccct gagcagcgag gaggaagaag aaatgggagg
181 tgccgcccag gagcctgaga gccttctgcc accctctgtg ctggaccagg ccagcgtcat
241 tgcggagcga tttgtcagca gcttctctcg gcggagcagc gtggcacagg aggacagcaa
301 gtccagtggc tttgggagcc cgcggctggt cagccggagc agcagcgtgc tcagcctgga
361 gggcagcgag aagggcctgg cccggcatgg cagtgccaca gactccctca gctgtcagct
421 ctccccagaa gtggacatca gtgtgggggt ggccacagag gacagccctt ctgtcaatgg
481 gatggagccc ccaagcccag gctgcccagt ggagcctgac cggtcttcct gcaagaagaa
541 ggaatcagca ctctccaccc gagaccggct gttgctagac aagattaaga gctattatga
601 aaatgcagaa caccatgatg caggcttcag cgtccgtcgc cgggagagcc tctcctacat
661 ccccaaagga ctggtaagaa actccatctc caggttcaac agccttcccc ggccagaccc
721 agagccagta cctccagtgg ggagcaagag acaggtgggc tcccggccga cttcgtgggc
781 cctgtttgag ctcccaggac caagccaggc agtcaaaggg gacccacctc ccatctcaga
841 tgctgagttc cgcccatctt cagaaattgt gaagatctgg gagggaatgg agtcttccgg
901 agggagccct gggaaggggc caggccaggg ccaggccaat ggctttgacc tgcatgagcc
961 actcttcatc ctggaggagc atgagctggg agccatcaca gaggagtcgg ccactgcctc
1021 cccggaaagc tcctctccca ctgaggggcg cagcccggcc cacctggccc gggagctgaa
1081 agagctggtg aaggagctga gcagcagtac ccagggggag ctggtggccc cactgcaccc
1141 ccgcatcgtg cagctctccc acgtaatgga cagccacgtg agcgagcgcg tcaagaacaa
1201 ggtctaccag ctggcccgcc agtacagcct ccggatcaag agcaacaagc cagtgatggc
1261 caggccacca ctgcagtggg aaaaggtggc ccctgagagg gatgggaaga gccccactgt
1321 gccctgtcta caggaagagg ctggagagcc attaggtggc aaaggtaaga ggaagccggt
1381 gctgtctcta tttgactatg agcagctgat ggcccaggag cacagccctc ccaagccctc
1441 ctcggctggg gagatgtcac cacagcgttt cttcttcaac ccgtctgctg tcagccagag
1501 gaccacctcg cctgggggcc ggccctccgc ccggagcccc ctcagcccca cagagacctt
1561 cagctggccc gacgtccgtg agctctgctc caagtatgcc tcccgcgatg aggcacgccg
1621 agcagggggc ggccggcccc gcggcccacc cgtcaacagg agccactcgg tgccggagaa
1681 catggtagag ccacctctgt cgggcagggt gggccgctgc cgcagcctga gcaccaagag
1741 gggccgggga ggcggagagg ctgcccaatc ccctgggcct ctgccccaga gcaagccgga
1801 tggaggcgag accctgtatg tcactgcaga cctcaccctg gaggacaacc ggcgggtgat
1861 tgtcatggag aagggacccc ttcccagccc cactgcaggg ctggaggaga gcagtggcca
1921 gggaccaagc tcaccggtgg ccctgctggg gcaggttcag gacttccagc agtctgcaga
1981 gtgccagccg aaggaagagg gttccaggga cccggcagac ccgagccagc agggcagagt
2041 gagaaacctt agagagaagt tccaggcctt gaactctgtc ggttgatgct gactcctggg
2101 ggagggagga gtcatgttgg aggttgggga agaacctggg catccttccc ctcaagcctg
2161 ggctcatgga gcccctgccc agggccctca ggtgggcgga aagtccatcc cctccgccct
2221 tcaggaagga tgctcccgtg tgcaggggtc tcctgcctgt gccatccact ggggctcgag
2281 acaatttccc actcacctgt gaggccggtg tggctgcttc ccttgtaaat agttgttctc
2341 tggtaagaag ccaaatattt aagctcactt cttcccagag agaggaagct ctgctcaggc
2401 ctccagcgtt ggctggccat ggccacagcc agatggagga gcccatcccc aggagactca
2461 ggcagtggcc tggagaggct ttgttctgta acggtgcctt ttcttagggt ccaggcagga
2521 atgaagccaa taatttattg ctttccattc tgtggtatga tgtgcgtgtg cgtgagtgtg
2581 tggcccctgt ttattcccct cctgtcaaga atgaagtgga ttcagttcag gtaaaaaaaa
2641 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//