LOCUS BC011392 2430 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1, mRNA
(cDNA clone MGC:9898 IMAGE:3869484), complete cds.
ACCESSION BC011392
VERSION BC011392.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2430)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2430)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 13 Row: i Column: 18.
FEATURES Location/Qualifiers
source 1..2430
/db_xref="H-InvDB:HIT000035236"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9898 IMAGE:3869484"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2430
/gene="PAPSS1"
/gene_synonym="ATPSK1"
/gene_synonym="PAPSS"
/gene_synonym="SK1"
/db_xref="GeneID:9061"
/db_xref="MIM:603262"
CDS 21..1832
/gene="PAPSS1"
/gene_synonym="ATPSK1"
/gene_synonym="PAPSS"
/gene_synonym="SK1"
/codon_start=1
/product="PAPSS1 protein"
/protein_id="AAH11392.1"
/db_xref="GeneID:9061"
/db_xref="MIM:603262"
/translation="MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGK
TTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADA
GLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGE
IKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYV
PENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLL
DGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEERCARQ
WGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNAD
AVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQ
HAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPH
PETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEFIS
GTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA"
misc_feature 108..584
/gene="PAPSS1"
/gene_synonym="ATPSK1"
/gene_synonym="PAPSS"
/gene_synonym="SK1"
/note="APS_kinase; Region: Adenylylsulfate kinase. Enzyme
that catalyses the phosphorylation of adenylylsulfate to
3'-phosphoadenylylsulfate. This domain contains an ATP
binding P-loop motif"
/db_xref="CDD:pfam01583"
misc_feature 837..1820
/gene="PAPSS1"
/gene_synonym="ATPSK1"
/gene_synonym="PAPSS"
/gene_synonym="SK1"
/note="ATP-sulfurylase; Region: ATP-sulfurylase. This
family consists of ATP-sulfurylase or sulfate
adenylyltransferase EC:2.7.7.4 some of which are part of a
bifunctional polypeptide chain associated with adenosyl
phosphosulphate (APS) kinase pfam01583. Both enzymes are
required for PAPS (phosphoadenosine-phosphosulfate)
synthesis from inorganic sulphate. ATP sulfurylase
catalyses the synthesis of adenosine-phosphosulfate APS
from ATP and inorganic sulphate"
/db_xref="CDD:pfam01747"
BASE COUNT 688 a 475 c 567 g 700 t
ORIGIN
1 ataacgcgca gaactgggga atgcagagag caaccaatgt cacctaccaa gcccatcatg
61 tcagcaggaa caagagaggt caggtggtgg ggaccagagg tggctttcgt ggttgcacag
121 tttggctaac aggcttgtct ggagcgggaa agactactgt gagcatggcc ttggaggagt
181 acctggtttg tcatggtatt ccatgctaca ctctggatgg tgacaatatt cgtcaaggtc
241 tcaataaaaa tcttggcttt agtcctgaag acagagaaga gaatgttcga cgcatcgcag
301 aagttgctaa actgtttgca gatgctggct tagtgtgcat cacaagtttc atatcacctt
361 acactcagga tcgcaacaat gcaaggcaaa ttcatgaagg tgcaagttta ccgttttttg
421 aagtatttgt tgatgctcct ctgcatgttt gtgaacagag ggatgtcaaa ggactctaca
481 aaaaagcccg ggcaggagaa attaaaggtt tcactgggat cgattctgaa tatgaaaagc
541 cagaggcccc tgagttggtg ctgaaaacag actcctgtga tgtaaatgac tgtgtccagc
601 aagttgtgga acttctacag gaacgggata ttgtacctgt ggatgcatct tatgaagtaa
661 aagaactata tgtgccagaa aataaacttc atttggcaaa aacagatgcg gaaacattac
721 cagcactgaa aattaataaa gtggatatgc agtgggtgca ggttttggca gaaggttggg
781 caaccccatt gaatggcttt atgagagaga gggagtactt gcagtgcctt cattttgatt
841 gtcttctgga tggaggtgtc attaacttgt cagtacctat agttctgact gcgactcatg
901 aagataaaga gaggctggac ggctgtacag catttgctct gatgtatgag ggccgccgtg
961 tggccattct tcgcaatcca gagttttttg agcacaggaa agaggagcgc tgtgccagac
1021 agtggggaac gacatgcaag aaccacccct atattaagat ggtgatggaa caaggagatt
1081 ggctgattgg aggagatctt caagtcttgg atcgagttta ttggaatgat ggtcttgatc
1141 agtatcgtct tactcctact gagctaaagc agaaatttaa agatatgaat gctgatgctg
1201 tctttgcatt tcaactacgc aacccagtgc acaatggaca tgccctgtta atgcaggata
1261 cccataagca acttctagag aggggctacc ggcgccctgt cctcctcctc caccctctgg
1321 gtggctggac aaaggatgac gatgttcctt tgatgtggcg tatgaagcag catgctgcag
1381 tgttggagga aggagttctg aatcctgaga cgacagtggt ggccatcttc ccatctccca
1441 tgatgtatgc tggaccaact gaggtccagt ggcattgcag agcacggatg gttgcaggag
1501 ccaactttta cattgttgga cgagaccctg ctggcatgcc tcatccagaa acagggaagg
1561 atctttatga gccaagtcat ggtgccaaag tgctgacgat ggcccctggt ttaatcactt
1621 tggaaatagt tccctttcga gttgcagctt acaacaagaa aaagaagcgt atggactact
1681 atgactctga acaccatgaa gactttgaat ttatttcagg aacacgaatg cgcaaacttg
1741 ctcgagaagg ccagaaacca cctgaaggtt tcatggctcc caaggcttgg accgtgctga
1801 cagaatacta caaatccttg gagaaagctt aggctgttaa cccagtcact ccacctttga
1861 cacattacta gtaacaagag gggaccacat agtctctgtt ggcatttctt tgtggtgtct
1921 gtctggacat gcttcctaaa aacagaccat tttccttaac ttgcatcagt tttggtctgc
1981 cttatgagtt ctgttttgaa caagtgtaac acactgatgg ttttaatgta tcttttccac
2041 ttattatagt tatattccta caatacaatt ttaaaattgt ctttttatat tatatttatg
2101 cttctgtgtc atgatttttt caagctgtta tattagttgt aaccagtagt attcacatta
2161 aatcttgctt tttttcccct taaaaaaaga aaaaaattac caaacaataa acttggctag
2221 accttgtttt gaggatttta caagaccttt gtagcgatta gatttttttt ctacattgaa
2281 aatagaaact gcttcctttc ttctttccag tcagctattg gtctttccag ctgttataat
2341 ctaaagtatt cttatgatct gtgtaagctc tgaatgaact tctttactca ataaaattaa
2401 ttttttggct tcttaaaaaa aaaaaaaaaa
//