LOCUS BC010549 1366 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2F (putative), mRNA
(cDNA clone MGC:18120 IMAGE:4153270), complete cds.
ACCESSION BC010549
VERSION BC010549.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1366)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1366)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 19 Row: p Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 18087856.
FEATURES Location/Qualifiers
source 1..1366
/db_xref="H-InvDB:HIT000034993"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:18120 IMAGE:4153270"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1366
/gene="UBE2F"
/gene_synonym="MGC18120"
/gene_synonym="NCE2"
/db_xref="GeneID:140739"
/db_xref="HGNC:HGNC:12480"
CDS 92..649
/gene="UBE2F"
/gene_synonym="MGC18120"
/gene_synonym="NCE2"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2F (putative)"
/protein_id="AAH10549.1"
/db_xref="GeneID:140739"
/db_xref="HGNC:HGNC:12480"
/translation="MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAEL
EANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLT
KIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIE
AAEHHLRDKEDFRNKVDDYIKRYAR"
BASE COUNT 391 a 279 c 310 g 386 t
ORIGIN
1 gcgtctcgca gcagccgccc ggaccgggca tggtgttggg cgccgggccc gcctcgcctg
61 tctcggggag cccagggtaa aggcagcagt aatgctaacg ctagcaagta aactgaagcg
121 tgacgatggt ctcaaagggt cccggacggc agccacagcg tccgactcga ctcggagggt
181 ttctgtgaga gacaaattgc ttgttaaaga ggttgcagaa cttgaagcta atttaccttg
241 tacatgtaaa gtgcattttc ctgatccaaa caagcttcat tgttttcagc taacagtaac
301 cccagatgag ggttactacc agggtggaaa atttcagttt gaaactgaag ttcccgatgc
361 gtacaacatg gtgcctccca aagtgaaatg cctgaccaag atctggcacc ccaacatcac
421 agagacaggg gaaatatgtc tgagtttatt gagagaacat tcaattgatg gcactggctg
481 ggctcccaca agaacattaa aggatgtcgt ttggggatta aactctttgt ttactgatct
541 tttgaatttt gatgatccac tgaatattga agctgcagaa catcatttgc gggacaagga
601 ggacttccgg aataaagtgg atgactacat caaacgttat gccagatgat aaaaggggac
661 gattgcaggc ccatggactg tgttacagtt tgtctctaac atgaaacagc aagaggtagc
721 cccctctccc gtcctcatgc tccctctcag tcccctggat tgccccagtc ctgtgaccat
781 gttgccctga agaagaccat cttcatgact gctcattgta gatggagaat tcaacataaa
841 tacagcaaga aaatgtgttt gggcttctga agagttgtct gcttacctta acatgtttac
901 ttttttgaac ttgtactgta taggctgttg gtgaaattct taagaagttg taatgaactc
961 aaaattgagg ccagagcttg ctttcccttt tcccaaacaa aattggtttt ctgcacaagc
1021 gatgctaatg atgtgttcag tgtaactcgc agattggcaa taagataccc gctacaaact
1081 gtgattggat gcaaaatctc ttagcttctt tcacgaatgt tggccctgcc tagatgttgt
1141 gaagcctccc agaatgcata gagtcattca ctgtagatct cttattgaaa tgcgtatttt
1201 atttaatgta agtatatttt ggaacagatt tgtaatttgt acaattcaat gctttaatta
1261 ttttttctat tctcatttag tttgtatttt cattgtatag agcagacaga aagatgttgg
1321 gtcaagcaac tattgaagag aaatacaaag aaaaaaaaaa aaaaaa
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