LOCUS BC010285 3236 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens neuroepithelial cell transforming 1, mRNA (cDNA clone
MGC:5367 IMAGE:3048980), complete cds.
ACCESSION BC010285
VERSION BC010285.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3236)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3236)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: d Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19923326.
FEATURES Location/Qualifiers
source 1..3236
/db_xref="H-InvDB:HIT000034882"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5367 IMAGE:3048980"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3236
/gene="NET1"
/gene_synonym="ARHGEF8"
/gene_synonym="NET1A"
/db_xref="GeneID:10276"
/db_xref="HGNC:HGNC:14592"
/db_xref="MIM:606450"
CDS 147..1775
/gene="NET1"
/gene_synonym="ARHGEF8"
/gene_synonym="NET1A"
/codon_start=1
/product="neuroepithelial cell transforming 1"
/protein_id="AAH10285.1"
/db_xref="GeneID:10276"
/db_xref="HGNC:HGNC:14592"
/db_xref="MIM:606450"
/translation="MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARV
TSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALW
SEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEE
LTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLA
AKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTP
KEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLL
CHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDV
RMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQ
SAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQM
AEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV"
BASE COUNT 966 a 615 c 736 g 919 t
ORIGIN
1 ctccaccgcg gcgagaggca tgggcacgtg gctgccgagg gtggccgagc tctgggaaga
61 aaagcccgtg tgcctctgca tagcgtcgct acagcgctga ctcggtgtgg attgattgga
121 aaggtttgag ggagtacttg ggaagcatgg tggcacatga tgagactgga ggtctcctac
181 ctattaaaag gaccatacga gtcctagatg tcaataacca gtccttcaga gaacaagagg
241 agccaagcaa taaaagagtt cgacctctgg ctcgtgtcac gtccttggca aatttaatct
301 ctcctgtaag aaatggagct gtcagacgtt ttggtcaaac aatacagtca tttacccttc
361 gtggtgacca cagatcccca gcctctgccc agaagttttc tagcaggtca acagtcccaa
421 cacccgccaa gagaaggagc agtgcactgt ggtcagagat gctggacatc accatgaagg
481 agtctctcac caccagggag atcagacggc aggaggcaat atatgaaatg tcccgaggtg
541 aacaggattt aattgaggat ctcaaacttg caagaaaggc ctaccatgac cccatgttaa
601 agttgtccat catgtcagaa gaggaactca cacatatatt tggtgatctg gactcttaca
661 tacctctgca tgaagatttg ttgacaagaa taggagaagc aaccaagcct gatggaacag
721 tggagcagat tggtcacatt ctcgtgagct ggttaccgcg cttgaatgcc tacagaggtt
781 actgtagtaa ccagctggca gccaaagctc ttcttgatca aaagaaacag gatccaagag
841 tccaagactt cctccagcga tgtctcgagt ctcccttcag tcgaaaacta gatctttgga
901 gtttcctaga tatccctcga agtcgcctag tcaaataccc tttactgtta aaagaaattc
961 ttaaacacac tccaaaagag caccctgatg ttcagcttct ggaggatgct atattgataa
1021 tacagggagt cctctctgat atcaacttga agaaaggtga atccgagtgc cagtattaca
1081 tcgacaagct ggagtacctg gatgaaaagc agagggaccc cagaatcgaa gcgagcaaag
1141 tgctgctgtg ccatggggag ctgcggagca agagtggaca taaactttac attttcctgt
1201 ttcaagacat cttggttctg actcggcccg tcacacggaa cgaacggcac tcttaccagg
1261 tttaccggca gccaatccca gtccaagagc tagtcctaga agacctgcag gatggagatg
1321 tgagaatggg aggctccttt cgaggagctt tcagtaactc agagaaagct aaaaatatct
1381 ttagaattcg cttccatgac ccctctccag cccagtctca cactctgcaa gccaatgacg
1441 tgttccacaa gcagcagtgg ttcaactgta ttcgagcggc cattgccccc ttccagtcgg
1501 caggcagtcc acctgagctg cagggcctgc cggagctgca cgaagagtgt gaggggaacc
1561 acccctctgc gaggaaactc acagcccaga ggagggcatc cacagtttcc agtgttactc
1621 aggtagaagt tgatgaaaac gcttacagat gtggctctgg catgcagatg gcagaggaca
1681 gcaagagctt aaagacacac cagacacagc ccggcatccg aagagcgagg gacaaagccc
1741 tttctggtgg caaacggaaa gagactttgg tgtagagaag gctctgtgtg ttaactgatg
1801 ggagagactg tttgtttata aatgtgtaca gttttgtttt ctcgtaaggg gagcatcata
1861 gggttacttt ataccagttg taacattttc attgtttttg gttgttcttt tttctttttt
1921 taatggcagc taaagatata cagattactg ttaaattgca gtcctttttt tttttaaaga
1981 tattttcttg aattatttag aacatggtaa gcctggtatt ttttaatcaa acaaaatatt
2041 tatgaaatgg gttttctctt aattctggat tcatcatggc tttctaatac caattgtaat
2101 atttacaata ttcaccaaaa cttagaattt tgcaaatgct ggaattctgc cagtgtttct
2161 ttgctaagcc ttgcatgcaa aatttgaaat tttaacattg gcacccaaaa cctacatgga
2221 atgtatgtct ggagtatttc aaactttaca ttgaaacata atttccttgg aaaacaaacc
2281 ataagcctga ggaggttttt atcaactgga atgctttata ttagtttgtt tttcactgta
2341 cattcctcat tttacattca tttaacctgc cgattattta atttttttat tgtaaagtag
2401 tttttagcat ttgcttttat ttttttactt tgatgccttt tcaaattggc atgtctttaa
2461 agtatttttc ttcctgatta aaaatgtgtg tgtatgtgtg tgtgtgtgtg tatatatata
2521 tattttttta aatcacatta attttaccaa gtgaaaccaa gccatactgt ttttgagcca
2581 attaagaaaa ttgccatttt taaagtgtag catttcaggg taaagaccca tgaaatggct
2641 tgatgtattc tagactactg aaagaaaacc acttcaaaga ttttgttgaa agttttagtg
2701 ttgtctgaaa tgcaagaggg aaggtgattg gtagtgagtt aaaagaaaaa gagaggaaaa
2761 gagagtagtt ttgtcttcaa gtaaaatgtc tggttgtgcc agacatttca caagtgtgaa
2821 aggagatagg agaagctcaa cttgagggcg tgtagtaagt tgtagaaggc tcgaggggac
2881 gtggacttat ttgccttggt ttgcaatacc tgcaaataat gagtttgaaa agaaacaatg
2941 aaatgtgtta aaaatttgac catattagat aaattttggt ggatttagtc ataagatgga
3001 aaaagactgg tgaatctttt attacaaaat gtttctgtta aaatgggatc atcatctttg
3061 aaagggggga ggaggagtaa aagcccgatt ataatggtga tcaattcaag tcagtgttga
3121 ctattctgtg aaatatattt ggccagtgga aatgataatc agaaaagact gtaaatagat
3181 ccatccaaat gatttctctg tacaaatgaa tgatactatt aaaaaaaaaa aaaaaa
//