LOCUS BC010276 2144 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens beclin 1, autophagy related, mRNA (cDNA clone MGC:5218
IMAGE:2901072), complete cds.
ACCESSION BC010276
VERSION BC010276.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2144)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2144)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: i Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19923741.
FEATURES Location/Qualifiers
source 1..2144
/db_xref="H-InvDB:HIT000034876"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5218 IMAGE:2901072"
/tissue_type="Cervix, carcinoma"
/clone_lib="NIH_MGC_12"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2144
/gene="BECN1"
/gene_synonym="ATG6"
/gene_synonym="beclin1"
/gene_synonym="VPS30"
/db_xref="GeneID:8678"
/db_xref="HGNC:HGNC:1034"
/db_xref="MIM:604378"
CDS 134..1486
/gene="BECN1"
/gene_synonym="ATG6"
/gene_synonym="beclin1"
/gene_synonym="VPS30"
/codon_start=1
/product="beclin 1, autophagy related"
/protein_id="AAH10276.1"
/db_xref="GeneID:8678"
/db_xref="HGNC:HGNC:1034"
/db_xref="MIM:604378"
/translation="MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTA
PLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGE
ASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQ
NYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAE
AERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHI
WHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYG
NHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCL
PYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK
"
BASE COUNT 625 a 438 c 535 g 546 t
ORIGIN
1 gctgaagaca gagcgatggt agttctggag gcctcgctcc ggggccgacc cgaggccaca
61 gtgcctccgc ggtagaccgg acttgggtga cgggctccgg gctcccgagg tgaagagcat
121 cgggggctga gggatggaag ggtctaagac gtccaacaac agcaccatgc aggtgagctt
181 cgtgtgccag cgctgcagcc agcccctgaa actggacacg agtttcaaga tcctggaccg
241 tgtcaccatc caggaactca cagctccatt acttaccaca gcccaggcga aaccaggaga
301 gacccaggag gaagagacta actcaggaga ggagccattt attgaaactc ctcgccagga
361 tggtgtctct cgcagattca tccccccagc caggatgatg tccacagaaa gtgccaacag
421 cttcactctg attggggagg catctgatgg cggcaccatg gagaacctca gccgaagact
481 gaaggtcact ggggaccttt ttgacatcat gtcgggccag acagatgtgg atcacccact
541 ctgtgaggaa tgcacagata ctcttttaga ccagctggac actcagctca acgtcactga
601 aaatgagtgt cagaactaca aacgctgttt ggagatctta gagcaaatga atgaggatga
661 cagtgaacag ttacagatgg agctaaagga gctggcacta gaggaggaga ggctgatcca
721 ggagctggaa gacgtggaaa agaaccgcaa gatagtggca gaaaatctcg agaaggtcca
781 ggctgaggct gagagactgg atcaggagga agctcagtat cagagagaat acagtgaatt
841 taaacgacag cagctggagc tggatgatga gctgaagagt gttgaaaacc agatgcgtta
901 tgcccagacg cagctggata agctgaagaa aaccaacgtc tttaatgcaa ccttccacat
961 ctggcacagt ggacagtttg gcacaatcaa taacttcagg ctgggtcgcc tgcccagtgt
1021 tcccgtggaa tggaatgaga ttaatgctgc ttggggccag actgtgttgc tgctccatgc
1081 tctggccaat aagatgggtc tgaaatttca gagataccga cttgttcctt acggaaacca
1141 ttcatatctg gagtctctga cagacaaatc taaggagctg ccgttatact gttctggggg
1201 gttgcggttt ttctgggaca acaagtttga ccatgcaatg gtggctttcc tggactgtgt
1261 gcagcagttc aaagaagagg ttgagaaagg cgagacacgt ttttgtcttc cctacaggat
1321 ggatgtggag aaaggcaaga ttgaagacac aggaggcagt ggcggctcct attccatcaa
1381 aacccagttt aactctgagg agcagtggac aaaagctctc aagttcatgc tgacgaatct
1441 taagtggggt cttgcttggg tgtcctcaca attttataac aaatgacttt tttccttagg
1501 gggaggtttg ccttaaaggc ttttaatttt gttttgtttg caaacatgtt ttaaattaaa
1561 ttcgggtaat attaaacagt acatgtttac aataccaaaa aagaaaaaat ccacaaaagc
1621 cactttattt taaaatatca tgtgacagat actttccaga gctacaacat gccatctata
1681 gttgccagcc ctggtcagtt ttgattctta accccatgga ctcctttccc tttcttctct
1741 gaaaaaaact aatttaaatt tgcttttctt ttttttaact gagttgaatt gagattgatg
1801 tgttttcact ggatttttat ctctctcaac ttcctgcact taacaatatg aaatagaaac
1861 ttttgtcttt actgagatga ggatatgttt gagatgcaca gttggataat gtgggaaaat
1921 gacatctaag ctttacctgg tcaccatgtg atgtgatcag atgcttgaaa tttaacactt
1981 ttcacttggt tcttatactg aatgccgact ctgctctgtg ttagagatat gaaatggtgt
2041 ttgatactgt ttgagacatt atggagagat ttaattattt gtaataaaag atttgctgca
2101 gtctgaaaac tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//