LOCUS BC010218 2742 bp mRNA linear HUM 03-NOV-2008
DEFINITION Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial, mRNA (cDNA
clone IMAGE:3449861), partial cds.
ACCESSION BC010218
VERSION BC010218.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2742)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2742)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 19 Row: c Column: 15.
FEATURES Location/Qualifiers
source 1..2742
/db_xref="H-InvDB:HIT000034863"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3449861"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2742
/gene="IARS2"
/db_xref="GeneID:55699"
/db_xref="HGNC:HGNC:29685"
CDS <1..2323
/gene="IARS2"
/codon_start=2
/product="IARS2 protein"
/protein_id="AAH10218.2"
/db_xref="GeneID:55699"
/db_xref="HGNC:HGNC:29685"
/translation="YKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLA
SLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVA
STLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGM
EDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEE
KLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMD
RRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQL
LPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGW
FQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEP
PYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSI
PVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDR
LYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGW
ISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQS
EETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIV
MPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK"
BASE COUNT 825 a 501 c 624 g 792 t
ORIGIN
1 ttacaaacct gtgttttggt ctccgtcatc taggactgca ttggctgaag cagaacttga
61 atataatcct gagcatgtca gtcgttcaat atatgtaaaa tttcctctct taaagccttc
121 tccaaaattg gcatctctta tagatggttc atctcctgtt agtattttgg tctggaccac
181 acaaccttgg acgattccag ccaatgaagc tgtttgctat atgcctgaat caaagtatgc
241 tgttgtgaaa tgttctaagt ctggagacct ctacgtactg gcggcagata aagtagcatc
301 tgttgcttct actttggaaa caacatttga gactatttca acactttcag gtgtagattt
361 ggaaaatggt acttgcagtc atccattaat tcctgataaa gcctctcctc ttttacctgc
421 aaatcatgtg accatggcaa aaggaacggg attggttcac acagccccag ctcatggtat
481 ggaagactac ggtgtagcgt ctcagcacaa cctgcccatg gattgtctag tggacgaaga
541 tggagttttc acagatgttg caggtcctga acttcaaaac aaggctgtcc ttgaagaggg
601 aactgatgtg gttataaaga tgcttcagac tgcaaagaat ttgttgaaag aggagaaatt
661 ggtgcatagc tatccgtatg actggaggac caagaaacct gtggttattc gtgccagcaa
721 gcagtggttt ataaacatca cggatattaa gactgcagcc aaggaattgt taaaaaaggt
781 gaaatttatt cctggatcag cactgaatgg catggttgaa atgatggaca ggcggccata
841 ttggtgtata tcaaggcaaa gagtttgggg tgttccaatt cctgtgtttc atcataagac
901 caaggatgaa tacttgatca acagccaaac cactgagcat attgttaaac tagtggaaca
961 acacggcagt gatatctggt ggactcttcc ccctgaacaa cttcttccaa aagaagtctt
1021 atctgaggtt ggtggccctg atgccttgga atatgtgcca ggtcaggata ttttggacat
1081 ctggtttgat agcggaactt catggtctta tgttcttcca ggtcctgacc aaagagcaga
1141 tttgtacttg gaaggaaaag accagctcgg gggttggttt cagtcatcct tattaacaag
1201 tgtggcagca aggaagagag caccttataa gacagtgatt gttcatggat ttacccttgg
1261 agaaaaggga gaaaagatgt ccaagtctct tgggaatgtc attcatcctg atgttgtcgt
1321 taatggagga caagatcaaa gcaaagagcc tccgtatggt gctgatgtcc ttcgctggtg
1381 ggtagctgat tccaatgtct tcaccgaagt tgcaattggc ccatccgtgc tcaatgctgc
1441 cagagatgat attagcaagc ttaggaatac acttcgcttt cttttgggaa atgtggctga
1501 tttcaaccca gaaacagatt ccatccctgt aaacgatatg tatgtcatag accagtacat
1561 gctacactta ctgcaggatt tggcaaacaa gattaccgaa ttatacaaac aatatgattt
1621 tggaaaagtt gttcggctgt tacggacgtt ttataccaga gagctctcta acttttattt
1681 cagtataatc aaagataggc tctattgtga aaaggaaaat gaccccaaac gacgctcttg
1741 tcagactgca ttagttgaaa ttttggatgt aatagttcgt tcttttgctc ccattcttcc
1801 tcacctggct gaagaggtgt tccagcacat accttatatt aaagagccca agagtgtttt
1861 ccgtactggg tggattagta ctagttctat ctggaaaaag cccgggttgg aagaagctgt
1921 ggagagtgcg tgtgcaatgc gagactcatt tcttggaagc atccctggca aaaatgcagc
1981 tgagtacaag gttatcactg tgatagaacc tggactgctt tttgagataa tagagatgct
2041 gcagtctgaa gagacttcca gcacctctca gttgaatgaa ttaatgatgg cttctgagtc
2101 aactttactg gctcaggaac cacgagagat gactgcagat gtaatcgagc ttaaagggaa
2161 attcctcatc aacttagaag gtggtgatat tcgtgaagag tcttcctata aagtaattgt
2221 catgccgact acgaaagaaa aatgcccccg ttgttggaag tatacagcgg agtcttcaga
2281 tacactgtgt cctcgatgtg cagaagttgt cagtggaaaa tagtattaac agctcactcg
2341 agcaagaacc ctcctgacag tactggctag aagtttggat ggattacaat ataggaaaga
2401 aagccaagat ttaggtaatg agtggatgag taaatggtgg aggatgggag tcaaaatcag
2461 aattatagaa gaagtatttc ctgtaactat agaaagaatt atgtatatat acatgcagaa
2521 atatatatgt gtgtgtgtat ctgtggatgg atatatgtat atctcttcct atatatatcc
2581 atagtggact tattcagaac atagatatgt attcagcttg tcttcaaata cggccaagca
2641 gaaaatgttt tatattttat aaatcatctt ttgactctgt atttaaattc tatgatactg
2701 aaaataaagg cattctggaa aaaaaaaaaa aaaaaaaaaa aa
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