LOCUS BC010032 2092 bp mRNA linear HUM 11-SEP-2007
DEFINITION Homo sapiens LAG1 homolog, ceramide synthase 2, mRNA (cDNA clone
MGC:19692 IMAGE:3506408), complete cds.
ACCESSION BC010032
VERSION BC010032.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2092)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2092)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC010032.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 27 Row: c Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32455257.
FEATURES Location/Qualifiers
source 1..2092
/db_xref="H-InvDB:HIT000034752"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19692 IMAGE:3506408"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2092
/gene="LASS2"
/gene_synonym="CerS2"
/gene_synonym="L3"
/gene_synonym="SP260"
/gene_synonym="TMSG1"
/db_xref="GeneID:29956"
/db_xref="HGNC:HGNC:14076"
/db_xref="MIM:606920"
CDS 46..1188
/gene="LASS2"
/gene_synonym="CerS2"
/gene_synonym="L3"
/gene_synonym="SP260"
/gene_synonym="TMSG1"
/codon_start=1
/product="LAG1 homolog, ceramide synthase 2"
/protein_id="AAH10032.1"
/db_xref="GeneID:29956"
/db_xref="HGNC:HGNC:14076"
/db_xref="MIM:606920"
/translation="MLQTLYDYFWWERLWLPVNLTWADLEDRDGRVYAKASDLYITLP
LALLFLIVRYFFELYVATPLAALLNIKEKTRLRAPPNATLEHFYLTSGKQPKQVEVEL
LSRQSGLSGRQVERWFRRRRNQDRPSLLKKFREASWRFTFYLIAFIAGMAVIVDKPWF
YDMKKVWEGYPIQSTIPSQYWYYMIELSFYWSLLFSIASDVKRKDFKEQIIHHVATII
LISFSWFANYIRAGTLIMALHDSSDYLLESAKMFNYAGWKNTCNNIFIVFAIVFIITR
LVILPFWILHCTLVYPLELYPAFFGYYFFNSMMGVLQLLHIFWAYLILRMAHKFITGK
LVEDERSDREETESSEGEEAAAGGGAKSRPLANGHPILNNNHRKND"
BASE COUNT 496 a 565 c 471 g 560 t
ORIGIN
1 agtacacgga gcagcggccc cggccccgcc aacgctgccg ccgggatgct ccagaccttg
61 tatgattact tctggtggga acgtctgtgg ctgcctgtga acttgacctg ggccgatcta
121 gaagaccgag atggacgtgt ctacgccaaa gcctcagatc tctatatcac gctgcccctg
181 gccttgctct tcctcatcgt tcgatacttc tttgagctgt acgtggctac accactggct
241 gccctcttga acataaagga gaaaactcgg ctgcgggcac ctcccaacgc caccttggaa
301 catttctacc tgaccagtgg caagcagccc aagcaggtgg aagtagagct tttgtcccgg
361 cagagcgggc tctctggccg ccaggtagag cgttggttcc gtcgccgccg caaccaggac
421 cggcccagtc tcctcaagaa gttccgagaa gccagctgga gattcacatt ttacctgatt
481 gccttcattg ccggcatggc cgtcattgtg gataaaccct ggttctatga catgaagaaa
541 gtttgggagg gatatcccat acagagcact atcccttccc agtattggta ctacatgatt
601 gaactttcct tctactggtc cctgctcttc agcattgcct ctgatgtcaa gcgaaaggat
661 ttcaaggaac agatcatcca ccatgtggcc accatcattc tcatcagctt ttcttggttt
721 gccaattaca tccgagctgg gactctaatc atggctctgc atgactcttc cgattacctg
781 ctggagtcag ccaagatgtt taactacgcg ggatggaaga acacctgcaa caacatcttc
841 atcgtcttcg ccattgtttt tatcatcacc cgactggtca tcctgccctt ctggatcctg
901 cattgcaccc tggtgtaccc actggagctc tatcctgcct tctttggcta ttacttcttc
961 aattccatga tgggagttct acagctgctg catatcttct gggcctacct cattttgcgc
1021 atggcccaca agttcataac tggaaagctg gtagaagatg aacgcagtga ccgggaagaa
1081 acagagagct cagaggggga ggaggctgca gctgggggag gagcaaagag ccggccccta
1141 gccaatggcc accccatcct caataacaac catcgtaaga atgactgaac cattattcca
1201 gctgcctccc agattaatgc ataaagccaa ggaactaccc cgctccctgc gctatagggt
1261 cactttaagc tctgggaaaa aggagaaagt gagaggagag ttctctgcat cctccctcct
1321 tgcttgtcac ccagttgcct ttaaaccaaa ttctaaccag cctatcccca ggtaggggga
1381 cgttggttat attctgttag agggggacgg tcgtattttc ctccctaccc gccaagtcat
1441 cctttctact gcttttgagg ccctccctca gctctctgtg ggtaggggtt acaattcaca
1501 ttccttattc tgagaatttg gccccagctg tttgcctttg actccctgac ctccagagcc
1561 agggttgtgc cttattgtcc catctgtggg cctcattctg ccaaagctgg accaaggcta
1621 acctttctaa gctccctaac ttgggccaga aaccaaagct gagcttttaa ctttctccct
1681 ctatgacaca aatgaattga gggtaggagg agggtgcaca taacccttac cctacctctg
1741 ccaaaaagtg ggggctgtac tggggactgc tcggatgatc tttcttagtg ctacttcttt
1801 cagctgtccc tgtagcgaca ggtctaagat ctgactgcct cctcctttct ctggcctctt
1861 cccccttccc tcttctcttc agctaggcta gctggtttgg agtagaatgg caactaattc
1921 taatttttat ttattaaata tttggggttt tggttttaaa gccagaatta cggctagcac
1981 ctagcatttc agcagaggga ccattttaga ccaaaatgta ctgttaatgg gttttttttt
2041 aaaattaaaa gattaaataa aaaatattaa ataaaaaaaa aaaaaaaaaa aa
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