LOCUS BC010019 1208 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens mediator complex subunit 8, mRNA (cDNA clone MGC:19641
IMAGE:2905623), complete cds.
ACCESSION BC010019
VERSION BC010019.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1208)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1208)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC010019.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 27 Row: a Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 48762707
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1208
/db_xref="H-InvDB:HIT000034742"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19641 IMAGE:2905623"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1208
/gene="MED8"
/gene_synonym="ARC32"
/db_xref="GeneID:112950"
/db_xref="HGNC:HGNC:19971"
/db_xref="MIM:607956"
CDS 331..870
/gene="MED8"
/gene_synonym="ARC32"
/codon_start=1
/product="MED8 protein"
/protein_id="AAH10019.3"
/db_xref="GeneID:112950"
/db_xref="HGNC:HGNC:19971"
/db_xref="MIM:607956"
/translation="MRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGA
DAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGL
SNWRPSGSSGPGQAGQPGAGTILAGTSGLQQVQMAGAPSQQQPMLSGVQMAQAGQPGK
MPSGIKTNIKSASMHPYQR"
BASE COUNT 348 a 276 c 311 g 273 t
ORIGIN
1 cagccgcctc ggccgccgca atgcagccct cctttctatg tgtcatactc gtttatctgg
61 gtgaccaacc tgttcctatt ggtgcagaga gaggagaagc agcttgaggc atcattagat
121 gcactgctga gtcaagtggc tgatctgaag aactctctgg ggagtttcat ttgcaagttg
181 gagaacgagt atggccggct gacctgcttt gccttgcttt ctggacagct gaacactctg
241 aacaaggtct tgaagcatga aaaaacaccg ctgttccgta accaggtcat cattcctctg
301 gtgttgtctc cagaccgaga tgaagatctc atgcggcaga ctgaaggacg ggtgcctgtt
361 ttcagccatg aggtagtccc tgaccatctg agaaccaagc ctgaccctga agtggaagaa
421 caggagaagc aactgacgac agatgctgcc cgcattggtg cagatgcagc ccagaagcag
481 atccagagct tgaataaaat gtgttcaaac cttctggaga aaatcagcaa agaggagcga
541 gaatcagaga gtggaggtct ccggccgaac aagcagacct ttaaccctac agacactaat
601 gccttggtgg cagctgttgc ctttgggaaa ggactatcta attggagacc ttcaggcagc
661 agtggtcctg gccaggcagg ccagccagga gctgggacga tccttgcagg aacctcagga
721 ttacagcagg tgcagatggc aggagctcca agccagcagc agccaatgct cagtggggta
781 caaatggctc aggcaggtca accagggaaa atgccaagtg gaataaaaac caacatcaag
841 tcggcttcca tgcatcccta ccagcggtga gtgtggctgg caacctcgac tccctggtgc
901 tctttgcaga gttgggcagt gaaattacct tttgctcaag gctcacctag atgggtacaa
961 taaaaagaac atgggctttc agcagcagac aaatcccact tccaccactg actagctgtg
1021 tgaccttgga caagtgacct aatttttctg agcctgtttc tcatttgtaa atggtgataa
1081 tacctacctc atagggttgt tgtgaggatt aaaatgagga aatgaatgta aagcacttag
1141 tacagtatat gaaataatgg gtattcaata aatgatagtt tctacaaaaa aaaaaaaaaa
1201 aaaaaaaa
//