LOCUS BC009989 3772 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens smoothened homolog (Drosophila), mRNA (cDNA clone
MGC:15193 IMAGE:4127774), complete cds.
ACCESSION BC009989
VERSION BC009989.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3772)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3772)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC009989.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 26 Row: h Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34147561.
FEATURES Location/Qualifiers
source 1..3772
/db_xref="H-InvDB:HIT000034718"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15193 IMAGE:4127774"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3772
/gene="SMO"
/gene_synonym="Gx"
/db_xref="GeneID:6608"
/db_xref="HGNC:HGNC:11119"
/db_xref="MIM:601500"
CDS 281..2644
/gene="SMO"
/gene_synonym="Gx"
/codon_start=1
/product="smoothened homolog (Drosophila)"
/protein_id="AAH09989.1"
/db_xref="GeneID:6608"
/db_xref="HGNC:HGNC:11119"
/db_xref="MIM:601500"
/translation="MAAARPARGPELPLLGLLLLLLLGDPGRGAASSGNATGPGPRSA
GGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEA
HGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERE
RGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQ
NPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFV
GSIGWLAQFMDGARREIVCRADGTMRLGEPTSNETLSCVIIFVIVYYALMAGVVWFVV
LTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFV
GYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETML
RLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKQPIPDCE
IKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGQSDDEPKRIKKS
KMIAKAFSKRHELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTK
MVARRGAILPQDISVTPVATPVPPEEQANLWLVEAEISPELQKRLGRKKKRRKRKKEV
CPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAGAWGAGDSCRQGAWTLVSNPFCPE
PSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF"
BASE COUNT 701 a 1145 c 1154 g 772 t
ORIGIN
1 gggctgaaga caacttggat tgcgaggcta gggcttgggg agtcgtgcat cccgttccgg
61 gcctccgcag cccaacatgg gccccgggtt ccaaagtttg cgaagttggg cgccgagggg
121 ccggggcgcg cggagcgtcc gggggggccc gggcccggat tctctgggcg cacaggtcgc
181 ctgagccgcc tccgcggccg ccgaggtcgt gcgtgtggcc ggggggctcc gaggagcagg
241 cgggggcgcc ggggcttttg ctgagttggc ggggttggcc atggccgctg cccgcccagc
301 gcgggggccg gagctcccgc tcctggggct gctgctgctg ctgctgctgg gggacccggg
361 ccggggggcg gcctcgagcg ggaacgcgac cgggcctggg cctcggagcg cgggcgggag
421 cgcgaggagg agcgcggcgg tgactggccc tccgccgccg ctgagccact gcggccgggc
481 tgccccctgc gagccgctgc gctacaacgt gtgcctgggc tcggtgctgc cctacggggc
541 cacctccaca ctgctggccg gagactcgga ctcccaggag gaagcgcacg gcaagctcgt
601 gctctggtcg ggcctccgga atgccccccg ctgctgggca gtgatccagc ccctgctgtg
661 tgccgtatac atgcccaagt gtgagaatga ccgggtggag ctgcccagcc gtaccctctg
721 ccaggccacc cgaggcccct gtgccatcgt ggagagggag cggggctggc ctgacttcct
781 gcgctgcact cctgaccgct tccctgaagg ctgcacgaat gaggtgcaga acatcaagtt
841 caacagttca ggccagtgcg aagtgccctt ggttcggaca gacaacccca agagctggta
901 cgaggacgtg gagggctgcg gcatccagtg ccagaacccg ctcttcacag aggctgagca
961 ccaggacatg cacagctaca tcgcggcctt cggggccgtc acgggcctct gcacgctctt
1021 caccctggcc acattcgtgg ctgactggcg gaactcgaat cgctaccctg ctgttattct
1081 cttctacgtc aatgcgtgct tctttgtggg cagcattggc tggctggccc agttcatgga
1141 tggtgcccgc cgagagatcg tctgccgtgc agatggcacc atgaggcttg gggagcccac
1201 ctccaatgag actctgtcct gcgtcatcat ctttgtcatc gtgtactacg ccctgatggc
1261 tggtgtggtt tggtttgtgg tcctcaccta tgcctggcac acttccttca aagccctggg
1321 caccacctac cagcctctct cgggcaagac ctcctacttc cacctgctca cctggtcact
1381 cccctttgtc ctcactgtgg caatccttgc tgtggcgcag gtggatgggg actctgtgag
1441 tggcatttgt tttgtgggct acaagaacta ccgataccgt gcgggcttcg tgctggcccc
1501 aatcggcctg gtgctcatcg tgggaggcta cttcctcatc cgaggagtca tgactctgtt
1561 ctccatcaag agcaaccacc ccgggctgct gagtgagaag gctgccagca agatcaacga
1621 gaccatgctg cgcctgggca tttttggctt cctggccttt ggctttgtgc tcattacctt
1681 cagctgccac ttctacgact tcttcaacca ggctgagtgg gagcgcagct tccgggacta
1741 tgtgctatgt caggccaatg tgaccatcgg gctgcccacc aagcagccca tccctgactg
1801 tgagatcaag aatcgcccga gccttctggt ggagaagatc aacctgtttg ccatgtttgg
1861 aactggcatc gccatgagca cctgggtctg gaccaaggcc acgctgctca tctggaggcg
1921 tacctggtgc aggttgactg ggcagagtga cgatgagcca aagcggatca agaagagcaa
1981 gatgattgcc aaggccttct ctaagcggca cgagctcctg cagaacccag gccaggagct
2041 gtccttcagc atgcacactg tgtcccacga cgggcccgtg gcgggcttgg cctttgacct
2101 caatgagccc tcagctgatg tctcctctgc ctgggcccag catgtcacca agatggtggc
2161 tcggagagga gccatactgc cccaggatat ttctgtcacc cctgtggcaa ctccagtgcc
2221 cccagaggaa caagccaacc tgtggctggt tgaggcagag atctccccag agctgcagaa
2281 gcgcctgggc cggaagaaga agaggaggaa gaggaagaag gaggtgtgcc cgctggcgcc
2341 gccccctgag cttcaccccc ctgcccctgc ccccagtacc attcctcgac tgcctcagct
2401 gccccggcag aaatgcctgg tggctgcagg tgcctgggga gctggggact cttgccgaca
2461 gggagcgtgg accctggtct ccaacccatt ctgcccagag cccagtcccc ctcaggatcc
2521 atttctgccc agtgcaccgg cccccgtggc atgggctcat ggccgccgac agggcctggg
2581 gcctattcac tcccgcacca acctgatgga cacagaactc atggatgcag actcggactt
2641 ctgagcctgc agagcaggac ctgggacagg aaagagagga accaatacct tcaaggctct
2701 tcttcctcac cgagcatgct tccctaggat cccgtcttcc agagaacctg tgggctgact
2761 gccctccgaa gagagttctg gatgtctggc tcaaagcagc aggactgtgg gaaagagcct
2821 aacatctcca tggggaggcc tcaccccagg gacagggccc tggagctcag ggtccttgtt
2881 tctgccctgc cagctgcagc ctggttggca gcatctgctc catcggggca gggggtatgc
2941 agagcttgtg gtggggcagg aacggtggag gcagaggtga cagttcccag agtgggcttt
3001 ggtggccagg gaggcagcct agcctatgtc tggcagatga gggctggctg ccgttttctg
3061 ggctgatggg tgccctttcc tggcagtctc agtccaaaag tgttgactgt gtcattagtc
3121 ctttgtctaa gtagggccag ggcaccgtat tcctctccca ggtgtttgtg gggctggaag
3181 gacctgctcc cacaggggcc atgtcctctc ttaataggtg gcactacccc aaacccatct
3241 tttgttctcc tatatcctcc ttctcctgtt ccatttcagt tcagtttcag cggtgccaac
3301 ctctttgcgt ttcctttttg ttgatgagga cccagagctg ctgcacacac tcacctctaa
3361 ccccctcccc tcgctgctgg gccccatctc cacaggagag actggttcgg ctctagggcc
3421 tcagtctgga gtgggatagg agcagtgagt gacaaagcct ctgaaagatg catcatctct
3481 tcctcacacc catttagtgg gggatgggtc ctctagactt gaggggctac cctgggaagc
3541 tgccgtagct tcagccaggc aagaaagctt ccttcaacct gcatagccgg tgggtgagga
3601 gattcccacc ttccatagcc tccaaacatg ttcccaaggc cccactttca agaatcagac
3661 agcaggaagc catagatgct ggctgggttc caggttatgg ggagaagaaa tacagtcaat
3721 aaaaggtttt tgtataaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//