LOCUS BC009419 1869 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens sphingosine kinase 1, mRNA (cDNA clone MGC:15040
IMAGE:3506924), complete cds.
ACCESSION BC009419
VERSION BC009419.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1869)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1869)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-JUN-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 23 Row: e Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41282001.
FEATURES Location/Qualifiers
source 1..1869
/db_xref="H-InvDB:HIT000034352"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15040 IMAGE:3506924"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1869
/gene="SPHK1"
/gene_synonym="SPHK"
/db_xref="GeneID:8877"
/db_xref="HGNC:HGNC:11240"
/db_xref="MIM:603730"
CDS 381..1577
/gene="SPHK1"
/gene_synonym="SPHK"
/codon_start=1
/product="sphingosine kinase 1"
/protein_id="AAH09419.1"
/db_xref="GeneID:8877"
/db_xref="HGNC:HGNC:11240"
/db_xref="MIM:603730"
/translation="MDPVVGCGRGLFGFVFSAGGPRGVLPRPCRVLVLLNPRGGKGKA
LQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVV
NGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRL
LSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALR
TYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLA
LLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLV
YVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMP
PPEEPL"
BASE COUNT 322 a 570 c 623 g 354 t
ORIGIN
1 gctccgcggc gccggctgcg aagttgagcg aaaagtttga ggccggaggg agcgaggccg
61 gggagtccgc tccagcgggg cgctccagtc cctcagacgt gggctgagct tgggacgagc
121 tgcgttccgc cccaggccac tgtagggaac ggcggtggcg cctccccagc aaaccggacc
181 gactgggtcc agccgccgca gggaatgaca ccggtgctcc tacagccacg gctccgggcg
241 gggaaggcga gccccacagc cggccctgcg acgccctcct gggcagcacc gataaggagc
301 tgaaggcagg agccgccgcc acgggcagcg cccccacagc gccagggacc ccctggcagc
361 gggagccgcg ggtcgaggtt atggatccag tggtcggttg cggacgtggc ctctttggtt
421 ttgttttctc agcgggcggc ccccggggcg tgctcccgcg gccctgccgc gtgctggtgc
481 tgctgaaccc gcgcggcggc aagggcaagg ccttgcagct cttccggagt cacgtgcagc
541 cccttttggc tgaggctgaa atctccttca cgctgatgct cactgagcgg cggaaccacg
601 cgcgggagct ggtgcggtcg gaggagctgg gccgctggga cgctctggtg gtcatgtctg
661 gagacgggct gatgcacgag gtggtgaacg ggctcatgga gcggcctgac tgggagaccg
721 ccatccagaa gcccctgtgt agcctcccag caggctctgg caacgcgctg gcagcttcct
781 tgaaccatta tgctggctat gagcaggtca ccaatgaaga cctcctgacc aactgcacgc
841 tattgctgtg ccgccggctg ctgtcaccca tgaacctgct gtctctgcac acggcttcgg
901 ggctgcgcct cttctctgtg ctcagcctgg cctggggctt cattgctgat gtggacctag
961 agagtgagaa gtatcggcgt ctgggggaga tgcgcttcac tctgggcacc ttcctgcgtc
1021 tggcagccct gcgcacctac cgcggccgac tggcctacct ccctgtagga agagtgggtt
1081 ccaagacacc tgcctccccc gttgtggtcc agcagggccc ggtagatgca caccttgtgc
1141 cactggagga gccagtgccc tctcactgga cagtggtgcc cgacgaggac tttgtgctag
1201 tcctggcact gctgcactcg cacctgggca gtgagatgtt tgctgcaccc atgggccgct
1261 gtgcagctgg cgtcatgcat ctgttctacg tgcgggcggg agtgtctcgt gccatgctgc
1321 tgcgcctctt cctggccatg gagaagggca ggcatatgga gtatgaatgc ccctacttgg
1381 tatatgtgcc cgtggtcgcc ttccgcttgg agcccaagga tgggaaaggt gtgtttgcag
1441 tggatgggga attgatggtt agcgaggccg tgcagggcca ggtgcaccca aactacttct
1501 ggatggtcag cggttgcgtg gagcccccgc ccagctggaa gccccagcag atgccaccgc
1561 cagaagagcc cttatgaccc ctgggccgcg ctgtgcctta gtgtctactt gcaggaccct
1621 tcctccttcc ctagggctgc agagcctgtc cacagctcct gtgggggtgg aggagactcc
1681 tctggagaag ggtgagaagg tggaggctat gctttggggg gacaggccag aatgaagtcc
1741 tgggtcagga gcccagctgg ctgggcccag ctgcctatgt aaggccttct agtttgttct
1801 gagaccccca ccccacgaac caaatccaaa taaagtgaca ttcccagcct gaaaaaaaaa
1861 aaaaaaaaa
//