LOCUS BC009026 1228 bp mRNA linear HUM 16-SEP-2003
DEFINITION Homo sapiens ligase III, DNA, ATP-dependent, mRNA (cDNA clone
IMAGE:4184595), complete cds.
ACCESSION BC009026
VERSION BC009026.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1228)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1228)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (30-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: l Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7710127
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1228
/db_xref="H-InvDB:HIT000034145"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4184595"
/tissue_type="Brain, glioblastoma with EGFR amplification"
/clone_lib="NCI_CGAP_Brn64"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1228
/gene="LIG3"
/db_xref="GeneID:3980"
/db_xref="MIM:600940"
CDS 51..950
/gene="LIG3"
/codon_start=1
/product="LIG3 protein"
/protein_id="AAH09026.1"
/db_xref="GeneID:3980"
/db_xref="MIM:600940"
/translation="MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLL
HGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGT
AGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDL
TELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFV
TSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADN
PSYNTKTQIIQDFLRKGSAGGVA"
misc_feature 327..521
/gene="LIG3"
/note="zf-PARP; Region: Poly(ADP-ribose) polymerase and
DNA-Ligase Zn-finger region. Poly(ADP-ribose) polymerase
is an important regulatory component of the cellular
response to DNA damage. The amino-terminal region of
Poly(ADP-ribose) polymerase consists of two PARP-type zinc
fingers. This region acts as a DNA nick sensor"
/db_xref="CDD:pfam00645"
BASE COUNT 377 a 288 c 296 g 267 t
ORIGIN
1 gagcaagttc cgaggcctac ggtgagcgcc ggagccggag aggcagctat atgtctttgg
61 ctttcaagat cttctttcca caaaccctcc gtgcactcag ccgaaaagaa ctgtgcctat
121 tccgaaaaca tcactggcgt gatgtaagac aattcagcca gtggtcagaa acagatctgc
181 ttcatggaca tcccctcttc ctgagaagaa agcctgttct atcattccag ggaagccatc
241 taagatcacg tgccacctac cttgttttct tgccagggtt gcatgtggga ctctgcagtg
301 gcccctgtga gatggctgag caacggttct gtgtggacta tgccaagcgt ggcacagctg
361 gctgcaaaaa atgcaaggaa aagattgtga agggcgtatg ccgaattggc aaagtggtgc
421 ccaatccctt ctcagagtct gggggtgata tgaaagagtg gtaccacatt aaatgcatgt
481 ttgagaaact agagcgggcc cgggccacca caaaaaaaat cgaggacctc acagagctgg
541 aaggctggga agagctggaa gataatgaga aggaacagat aacccagcac attgcagatc
601 tgtcttctaa ggcagcaggt acaccaaaga agaaagctgt tgtccaggct aagttgacaa
661 ccactggcca ggtgacttct ccagtgaaag gcgcctcatt tgtcaccagt accaatcccc
721 ggaaattttc tggcttttca gccaagccca acaactctgg ggaagccccc tcgagcccca
781 cccctaagag aagtctgtct tcaagcaaat gtgaccccag gcataaggac tgtctgctac
841 gggagtttcg aaagttatgc gccatggtgg ccgataatcc tagctacaac acgaagaccc
901 agatcatcca ggacttcctt cggaaaggct cagcaggagg tgtggcatga gcatcctgaa
961 taggcctttc ctccggagag ctcaaggcca tggctgcctt cctgggcatg tgagctgtat
1021 agttgcccgg gatcccatgc ttggtttaat gctgtactgt agtgctatct ggaaattctt
1081 aataattttg aacaaggggc ctcgcatttg cattttgcac tgtactccac aaattatgta
1141 gccagttttt ctcagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1201 aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//