LOCUS BC009011 1696 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens fatty acid desaturase 2, mRNA (cDNA clone MGC:17124
IMAGE:4181037), complete cds.
ACCESSION BC009011
VERSION BC009011.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1696)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1696)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (30-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: k Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14141180.
FEATURES Location/Qualifiers
source 1..1696
/db_xref="H-InvDB:HIT000034138"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:17124 IMAGE:4181037"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1696
/gene="FADS2"
/gene_synonym="D6D"
/gene_synonym="DES6"
/gene_synonym="FADSD6"
/gene_synonym="LLCDL2"
/gene_synonym="SLL0262"
/gene_synonym="TU13"
/db_xref="GeneID:9415"
/db_xref="MIM:606149"
CDS 93..1253
/gene="FADS2"
/gene_synonym="D6D"
/gene_synonym="DES6"
/gene_synonym="FADSD6"
/gene_synonym="LLCDL2"
/gene_synonym="SLL0262"
/gene_synonym="TU13"
/codon_start=1
/product="FADS2 protein"
/protein_id="AAH09011.1"
/db_xref="GeneID:9415"
/db_xref="MIM:606149"
/translation="MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVY
NITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQD
HGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIP
TLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHF
QHHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIP
MYFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNFIRFLESHWF
VWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEHQ"
misc_feature 150..386
/gene="FADS2"
/gene_synonym="D6D"
/gene_synonym="DES6"
/gene_synonym="FADSD6"
/gene_synonym="LLCDL2"
/gene_synonym="SLL0262"
/gene_synonym="TU13"
/note="heme_1; Region: Heme/Steroid binding domain. This
family includes heme binding domains from a diverse range
of proteins. This family also includes proteins that bind
to steroids. The family includes progesterone receptors.
Many members of this subfamily are membrane anchored by an
N-terminal transmembrane alpha helix. This family also
includes a domain in some chitin synthases. There is no
known ligand for this domain in the chitin synthases"
/db_xref="CDD:pfam00173"
misc_feature 585..1247
/gene="FADS2"
/gene_synonym="D6D"
/gene_synonym="DES6"
/gene_synonym="FADSD6"
/gene_synonym="LLCDL2"
/gene_synonym="SLL0262"
/gene_synonym="TU13"
/note="FA_desaturase; Region: Fatty acid desaturase"
/db_xref="CDD:pfam00487"
BASE COUNT 372 a 504 c 455 g 365 t
ORIGIN
1 ccgcacacac cggctgggag gcagccgtct gtgcagcgag cagccggcgc ggggaggccg
61 cagtgcacgg ggcgtcacag tcggcaggca gcatggggaa gggagggaac cagggcgagg
121 gggccgccga gcgcgaggtg tcggtgccca ccttcagctg ggaggagatt cagaagcata
181 acctgcgcac cgacaggtgg ctggtcattg accgcaaggt ttacaacatc accaaatggt
241 ccatccagca cccggggggc cagcgggtca tcgggcacta cgctggagaa gatgcaacgg
301 atgccttccg cgccttccac cctgacctgg aattcgtggg caagttcttg aaacccctgc
361 tgattggtga actggccccg gaggagccca gccaggacca cggcaagaac tcaaagatca
421 ctgaggactt ccgggccctg aggaagacgg ctgaggacat gaacctgttc aagaccaacc
481 acgtgttctt cctcctcctc ctggcccaca tcatcgccct ggagagcatt gcatggttca
541 ctgtctttta ctttggcaat ggctggattc ctaccctcat cacggccttt gtccttgcta
601 cctctcaggc ccaagctgga tggctgcaac atgattatgg ccacctgtct gtctacagaa
661 aacccaagtg gaaccacctt gtccacaaat tcgtcattgg ccacttaaag ggtgcctctg
721 ccaactggtg gaatcatcgc cacttccagc accacgccaa gcctaacatc ttccacaagg
781 atcccgatgt gaacatgctg cacgtgtttg ttctgggcga atggcagccc atcgagtacg
841 gcaagaagaa gctgaaatac ctgccctaca atcaccagca cgaatacttc ttcctgattg
901 ggccgccgct gctcatcccc atgtatttcc agtaccagat catcatgacc atgatcgtcc
961 ataagaactg ggtggacctg gcctgggccg tcagctacta catccggttc ttcatcacct
1021 acatcccttt ctacggcatc ctgggagccc tccttttcct caacttcatc aggttcctgg
1081 agagccactg gtttgtgtgg gtcacacaga tgaatcacat cgtcatggag attgaccagg
1141 aggcctaccg tgactggttc agtagccagc tgacagccac ctgcaacgtg gagcagtcct
1201 tcttcaacga ctggttcagt ggacacctta acttccagat tgagcaccag tgagcgcggg
1261 gctgcgggga ggcggggaga cccacagcgg gagggaagtg gccgctatcc cactgggcag
1321 aatgcagaat gtgggggcca cctctttgtc tctctgacaa ggtctctgcc caatatagag
1381 caaggctccc agccagcccc gctgaggtca ggagaggggc tcccctattc catctccctg
1441 agggtcatcc tgtgcccctc atgctgtgcc acttggtcat agtggttacc acctccctcc
1501 atgtgggcct gggactttgt agttatggag cctgcacacg cacctcatga ccctgtgagg
1561 ctagcagggc agggctacgt agcttgcctg gaacatcggc cgagggactg ccctgctgcc
1621 ccttgtcctt gcttcacctc ttgtcactgt tttttttttt ttttattaaa aaataaaaaa
1681 tgtaaaaaaa aaaaaa
//