LOCUS BC009009 1777 bp mRNA linear HUM 16-SEP-2003
DEFINITION Homo sapiens phospholipase C, beta 2, mRNA (cDNA clone
IMAGE:3446252), complete cds.
ACCESSION BC009009
VERSION BC009009.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1777)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1777)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (30-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: a Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1777
/db_xref="H-InvDB:HIT000034136"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3446252"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1777
/gene="PLCB2"
/db_xref="GeneID:5330"
/db_xref="MIM:604114"
CDS 239..838
/gene="PLCB2"
/codon_start=1
/product="PLCB2 protein"
/protein_id="AAH09009.1"
/db_xref="GeneID:5330"
/db_xref="MIM:604114"
/translation="MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKG
YYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVV
SGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLN
SEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKPGGAR"
BASE COUNT 427 a 468 c 456 g 426 t
ORIGIN
1 ggcaggcagg gcgggtggga ggagggtgat ttacaaggca gatgggcctc tggctacagc
61 tcagtcctgc actcagccag ggccacccca ggggctataa gagcaactag atttctggag
121 cagctcgggg atgggtgcca tttgagccca gcttggctcc ccctcctggc tggcctcctt
181 cctgcccttc tgcctgcctg tgtctgctga gattctgcaa agaggaacgc ttggcaccat
241 gtctctgctc aaccctgtcc tgctgccccc caaggtgaag gcctatctga gccaagggga
301 gcgcttcatc aaatgggatg atgaaactac agttgcctct ccagttatcc tccgtgtgga
361 tcctaagggc tactacttat actggacgta tcaaagtaag gagatggagt ttctggatat
421 caccagcatc cgggatactc gctttgggaa gtttgccaag atgcccaaga gccagaagct
481 ccgggacgtc ttcaacatgg actttcctga taacagtttc ctgctgaaga cactcacggt
541 ggtgtccggc ccggacatgg tggacctcac cttccacaac ttcgtctcct acaaggagaa
601 cgtgggcaag gcctgggctg aggacgtact ggccctagtc aaacatccgc tgacggccaa
661 cgcctcccgc agcaccttcc tggacaagat ccttgtgaag ctcaagatgc agctcaactc
721 tgaagggaag attccggtga agaacttttt ccagatgttt cctgctgacc gcaagcgggt
781 ggaagctgct ctcagtgcct gccacctccc caaaggcaaa cctggaggag cgagataagg
841 tcggcagtgg caggcggcag tctgcaggga tctggaagtc tgcagcgtgt cgagggagag
901 gaactgaacc aaaggcggaa gctgccaaga tgcagacttc tgctctgtac agaaaagcat
961 ttttccctgt ctttttagtt aaataattaa tttttaatta caagttgtaa gcagggtcac
1021 cccatgaagc aacttgtgtg aattacaaaa aggagccctc tgggctgata tggccctgag
1081 ctaggcttca agcctggcca atggagcgaa tctcagcccc tgtccctgct tggccagtgc
1141 tcttgtgtgg gtcccagcct ataccccatg gagtattttc tcctggtaag aatttggaac
1201 gttataaata aataaaacca cgacccccat tgatcacctg cccacatgaa tcccaggagg
1261 agcatttacc ccacagccac agctctgcag agttggtgac tccttgatgc agtcagtcac
1321 agggatccaa atgtggcgtt ggggagaggt aggaatgggc tcctgcggct cccctacagc
1381 cagcaacact tacctgtggt cagcacacat ccaccctccc tctctcctgc cgcctgagct
1441 cctcccttgg ctaagtcctc cttatctatc tgctttcctt gtgtctcagt agaggctact
1501 gacccaactt tgcttggagc tgaagctcca gagacaggga ggggtttggg gagatccagt
1561 tagactctcc tgctcagacc ctctgctcca aactaagact gatgttggcc ttccagtgtg
1621 attctgaaag acatttaatg ttttttttgg ggggggaagg aatgcaaagg tgcagaatcc
1681 ctgaatacca caaatgtact tttcatttct atttctatag atttgcctat tctggtggta
1741 aagtaaatga aatcataaat ataaaaaaaa aaaaaaa
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