LOCUS BC008771 2692 bp mRNA linear HUM 16-SEP-2003
DEFINITION Homo sapiens MAP/microtubule affinity-regulating kinase 2, mRNA
(cDNA clone IMAGE:3139103), partial cds.
ACCESSION BC008771
VERSION BC008771.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2692)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2692)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC008771.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 4 Row: o Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 9845488.
FEATURES Location/Qualifiers
source 1..2692
/db_xref="H-InvDB:HIT000033958"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3139103"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2692
/gene="MARK2"
/gene_synonym="EMK1"
/db_xref="GeneID:2011"
/db_xref="MIM:600526"
CDS <1..2338
/gene="MARK2"
/gene_synonym="EMK1"
/codon_start=2
/product="MARK2 protein"
/protein_id="AAH08771.2"
/db_xref="GeneID:2011"
/db_xref="MIM:600526"
/translation="LNERDTEQPTLGHLDSKPSSKSNMIRGRNSATSADEQPHIGNYR
LLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNI
VKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKF
IVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDG
PEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLI
LNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQD
SLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSAN
PKQRRFSDQAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPG
LERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPGSRASTASA
SAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVPVASPSAHNIS
SSGGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSI
FSKFTSKFVRRNLSFRFARRNLNEPESKDRVETLRPHVVGSGGNDKEKEEFREAKPRS
LRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEM
EVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL"
misc_feature 128..883
/gene="MARK2"
/gene_synonym="EMK1"
/note="S_TKc; Region: Serine/Threonine protein kinases,
catalytic domain"
/db_xref="CDD:smart00220"
misc_feature 2207..2335
/gene="MARK2"
/gene_synonym="EMK1"
/note="KA1; Region: Kinase associated domain 1"
/db_xref="CDD:pfam02149"
BASE COUNT 669 a 783 c 729 g 511 t
ORIGIN
1 gctgaacgag agggacacgg agcagcccac cttgggacac cttgactcca agcccagcag
61 taagtccaac atgattcggg gccgcaactc agccacctct gctgatgagc agccccacat
121 tggaaactac cggctcctca agaccattgg caagggtaat tttgccaagg tgaagttggc
181 ccgacacatc ctgactggga aagaggtagc tgtgaagatc attgacaaga ctcaactgaa
241 ctcctccagc ctccagaaac tattccgcga agtaagaata atgaaggttt tgaatcatcc
301 caacatagtt aaattatttg aagtgattga gactgagaaa acgctctacc ttgtcatgga
361 gtacgctagt ggcggagagg tatttgatta cctagtggct catggcagga tgaaagaaaa
421 agaggctcga gccaaattcc gccagatagt gtctgctgtg cagtactgtc accagaagtt
481 tattgtccat agagacttaa aggcagaaaa cctgctcttg gatgctgata tgaacatcaa
541 gattgcagac tttggcttca gcaatgaatt cacctttggg aacaagctgg acaccttctg
601 tggcagtccc ccttatgctg ccccagaact cttccagggc aaaaaatatg atggacccga
661 ggtggatgtg tggagcctag gagttatcct ctatacactg gtcagcggat ccctgccttt
721 tgatggacag aacctcaagg agctgcggga acgggtactg aggggaaaat accgtattcc
781 attctacatg tccacggact gtgaaaacct gcttaagaaa tttctcattc ttaatcccag
841 caagagaggc actttagagc aaatcatgaa agatcgatgg atgaatgtgg gtcacgaaga
901 tgatgaacta aagccttacg tggagccact ccctgactac aaggaccccc ggcggacaga
961 gctgatggtg tccatgggtt atacacggga agagatccag gactcgctgg tgggccagag
1021 atacaacgag gtgatggcca cctatctgct cctgggctac aagagctccg agctggaagg
1081 cgacaccatc accctgaaac cccggccttc agctgatctg accaatagca gcgccccatc
1141 cccatcccac aaggtacagc gcagcgtgtc ggccaatccc aagcagcggc gcttcagcga
1201 ccaggcagct ggtcctgcca ttcccacctc taattcttac tctaagaaga ctcagagtaa
1261 caacgcagaa aataagcggc ctgaggagga ccgggagtca gggcggaaag ccagcagcac
1321 agccaaggtg cctgccagcc ccctgcccgg tctggagagg aagaagacca ccccaacccc
1381 ctccacgaac agcgtcctct ccaccagcac aaatcgaagc aggaattccc cacttttgga
1441 gcgggccagc ctcggtcagg cctccatcca gaatggcaaa gacagcctaa ccatgccagg
1501 gtcccgggcc tccacggctt ctgcttctgc cgcagtctct gcggcccggc cccgccagca
1561 ccagaaatcc atgtcggcct ccgtgcaccc caacaaggcc tctgggctgc cccccacgga
1621 gagtaactgt gaggtgccgc ggcccagcac agccccccag cgtgtccctg ttgcctcccc
1681 atccgcccac aacatcagca gcagtggtgg agccccagac cgaactaact tcccccgggg
1741 tgtgtccagc cgaagcacct tccatgctgg gcagctccga caggtgcggg accagcagaa
1801 tttgccctac ggtgtgaccc cagcctctcc ctctggccac agccagggcc ggcggggggc
1861 ctctgggagc atcttcagca agttcacctc caagtttgta cgcagaaatc tgtctttcag
1921 gtttgccaga aggaacctga atgaacctga aagcaaagac cgagtggaga cgctcagacc
1981 tcacgtggtg ggcagtggcg gcaacgacaa agaaaaggaa gaatttcggg aggccaagcc
2041 ccgctccctc cgcttcacgt ggagtatgaa gaccacgagc tccatggagc ccaacgagat
2101 gatgcgggag atccgcaagg tgctggacgc gaacagctgc cagagcgagc tgcatgagaa
2161 gtacatgctg ctgtgcatgc acggcacgcc gggccacgag gacttcgtgc agtgggagat
2221 ggaggtgtgc aaactgccgc ggctctctct caacggggtt cgatttaagc ggatatcggg
2281 cacctccatg gccttcaaaa acattgcctc caaaatagcc aacgagctga agctttaaca
2341 ggctgccagg agcgggggcg gcgggggcgg gccagctgga cgggctgccg gccgctgcgc
2401 cgccccacct gggcgagact gcagcgatgg attggtgtgt ctcccctgct ggcacttctc
2461 ccctccctgg cccttctcag ttttctctta catgtttgtg gggggtggga gattgttctc
2521 cagcccccca cattcacccc tgcccagaga ttcccccttc tcctctcccc tactggaggc
2581 aaaggaaggg gagggtggat gggggggcag ggctccccct cggtactgcg gttgcacaga
2641 gtatttcgcc taaaccaaga aattttttat taccaaaaaa aaaaaaaaaa aa
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