LOCUS BC008740 2140 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens U2 small nuclear RNA auxiliary factor 2, mRNA (cDNA
clone MGC:1210 IMAGE:3346087), complete cds.
ACCESSION BC008740
VERSION BC008740.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2140)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2140)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC008740.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: a Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 60279266.
FEATURES Location/Qualifiers
source 1..2140
/db_xref="H-InvDB:HIT000033935"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1210 IMAGE:3346087"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2140
/gene="U2AF2"
/gene_synonym="U2AF65"
/db_xref="GeneID:11338"
/db_xref="HGNC:HGNC:23156"
/db_xref="MIM:191318"
CDS 60..1475
/gene="U2AF2"
/gene_synonym="U2AF65"
/codon_start=1
/product="U2 small nuclear RNA auxiliary factor 2"
/protein_id="AAH08740.1"
/db_xref="GeneID:11338"
/db_xref="HGNC:HGNC:23156"
/db_xref="MIM:191318"
/translation="MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSR
DRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHI
TPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGI
TEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGII
FQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVK
ELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQR
ASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYE
EIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKF
ANRVVVTKYCDPDSYHRRDFW"
BASE COUNT 454 a 640 c 639 g 407 t
ORIGIN
1 ctggagcggg cggcaaggcg aggcgaaagc tgcacagggc cctacgcggc cgcctcagca
61 tgtcggactt cgacgagttc gagcggcagc tcaacgagaa taaacaagag cgggacaagg
121 agaaccggca tcggaagcgc agccacagcc gctctcggag ccgggaccgc aaacgccgga
181 gccggagccg cgaccggcgc aaccgggacc agcggagcgc ctcccgggac aggcgacgac
241 gcagcaaacc tttgaccaga ggcgctaaag aggagcacgg tggactgatt cgttcccccc
301 gccacgagaa gaagaagaag gtccgtaaat actgggacgt gccaccccca ggctttgagc
361 acatcacccc aatgcagtac aaggccatgc aagctgcggg tcagattcca gccactgctc
421 ttctccccac catgacccct gacggtctgg ctgtgacccc aacgccggtg cccgtggtcg
481 ggagccagat gaccagacaa gcccggcgcc tctacgtggg caacatcccc tttggcatca
541 ctgaggaggc catgatggat ttcttcaacg cccagatgcg cctggggggg ctgacccagg
601 cccctggcaa cccagtgttg gctgtgcaga ttaaccagga caagaatttt gcctttttgg
661 agttccgctc agtggacgag actacccagg ctatggcctt tgatggcatc atcttccagg
721 gccagtcact aaagatccgc aggcctcacg actaccagcc gcttcctggc atgtcagaga
781 acccctccgt ctatgtgcct ggggttgtgt ccactgtggt ccccgactct gcccacaagc
841 tgttcatcgg gggcttaccc aactacctga acgatgacca ggtcaaagag ctgctgacat
901 cctttgggcc cctcaaggcc ttcaacctgg tcaaggacag tgccacgggg ctctccaagg
961 gctacgcctt ctgtgagtac gtggacatca acgtcacgga tcaggccatt gcggggctga
1021 acggcatgca gctgggggat aagaagctgc tggtccagag ggcgagtgtg ggagccaaga
1081 atgccacgct gagcaccatc aatcagacgc ctgtgaccct gcaagtgccg ggcttgatga
1141 gctcccaggt gcagatgggc ggccacccga ctgaggtcct gtgcctcatg aacatggtgc
1201 tgcctgagga gctgctggac gacgaggagt atgaggagat cgtggaggat gtgcgggacg
1261 agtgcagcaa gtacgggctt gtcaagtcca tcgagatccc ccggcctgtg gacggcgtcg
1321 aggtgcccgg ctgcggaaag atctttgtgg agttcacctc tgtgtttgac tgccagaaag
1381 ccatgcaggg cctgacgggc cgcaagttcg ccaacagagt ggttgtcaca aaatactgtg
1441 accccgactc ttatcaccgc cgggacttct ggtagaggcg gctgggggag ggtgggggca
1501 gggctggctg ggggcttctc cccactcccg cccccccctt atccccctct gaagacgatg
1561 ggcagaggag tgacagccgc agacacacga cagccggcag caactggaat ggcagcaatt
1621 aagggtgggg ggcgggggtt ggggggttgg ggggttaggg cagggagggg actggggaag
1681 tgcgcacaca gcccacacag acaacacgca cccacacaga cacagaggga aggggttggg
1741 atggggacag ggtgcacagc agggcggggt aggaccccag cccctcccaa aacagcctct
1801 ccttctccca tagacccctt tcttctcccc ttccccacgg taggaacata gcgtgtttat
1861 attttatggc caaactattt tgaattttgt tgtccggccc tcagtgccct gccctctccc
1921 ttaccaggac cacagctctg ttccttcggc ctctggtcct ctctggtccc ctcctgggtt
1981 tcttacgtag ttgatttttc ctctttagtc tcccccgacc tgcgcccagc cccgtggccc
2041 ctgcccctct cctactctct gtggcagttt catatttgct aagacgaatt tgctcattaa
2101 acattttgtt gtattttact ttaaaaaaaa aaaaaaaaaa
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