LOCUS BC008489 1798 bp mRNA linear HUM 02-DEC-2006
DEFINITION Homo sapiens NUF2, NDC80 kinetochore complex component, homolog (S.
cerevisiae), mRNA (cDNA clone MGC:14815 IMAGE:4134468), complete
cds.
ACCESSION BC008489
VERSION BC008489.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1798)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1798)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 21 Row: l Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22027505.
FEATURES Location/Qualifiers
source 1..1798
/db_xref="H-InvDB:HIT000033825"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:14815 IMAGE:4134468"
/tissue_type="Bone marrow, chronic myelogenous leukemia"
/clone_lib="NIH_MGC_54"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1798
/gene="NUF2"
/gene_synonym="NUF2R"
/db_xref="GeneID:83540"
/db_xref="HGNC:HGNC:14621"
CDS 88..1482
/gene="NUF2"
/gene_synonym="NUF2R"
/codon_start=1
/product="NUF2, NDC80 kinetochore complex component,
homolog (S. cerevisiae)"
/protein_id="AAH08489.1"
/db_xref="GeneID:83540"
/db_xref="HGNC:HGNC:14621"
/translation="METLSFPRYNVAEIVIHIRNKILTGADGKNLTKNDLYPNPKPEV
LHMIYMRALQIVYGIRLEHFYMMPVNSEVMYPHLMEGFLPFSNLVTHLDSFLPICRVN
DFETADILCPKAKRTSRFLSGIINFIHFREACRETYMEFLWQYKSSADKMQQLNAAHQ
EALMKLERLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQKTIVLQEGNSQKKSNISE
KTKRLNELKLSVVSLKEIQESLKTKIVDSPEKLKNYKEKMKDTVQKLKNARQEVVEKY
EIYGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASILKESLNLEDQIESDESELKKL
KTEENSFKRLMIVKKEKLATAQFKINKKHEDVKQYKRTVIEDCNKVQEKRGAVYERVT
TINQEIQKIKLGIQQLKDAAEREKLKSQEIFLNLKTALEKYHDGIEKAAEDSYAKIDE
KTAELKRKMFKMST"
BASE COUNT 673 a 290 c 352 g 483 t
ORIGIN
1 gatttttgac tttgcttgta gctgctcccc gaactcgccg tcttcctgtc ggcggccggc
61 actgtagatt aacaggaaac ttccaagatg gaaactttgt ctttccccag atataatgta
121 gctgagattg tgattcatat tcgcaataag atcttaacag gagctgatgg taaaaacctc
181 accaagaatg atctttatcc aaatccaaag cctgaagtct tgcacatgat ctacatgaga
241 gccttacaaa tagtatatgg aattcgactg gaacattttt acatgatgcc agtgaactct
301 gaagtcatgt atccacattt aatggaaggc ttcttaccat tcagcaattt agttactcat
361 ctggactcat ttttgcctat ctgccgggtg aatgactttg agactgctga tattctatgt
421 ccaaaagcaa aacggacaag tcggttttta agtggcatta tcaactttat tcacttcaga
481 gaagcatgcc gtgaaacgta tatggaattt ctttggcaat ataaatcctc tgcggacaaa
541 atgcaacagt taaacgccgc acaccaggag gcattaatga aactggagag acttgattct
601 gttccagttg aagagcaaga agagttcaag cagctttcag atggaattca ggagctacaa
661 caatcactaa atcaggattt tcatcaaaaa acgatagtgc tgcaagaggg aaattcccaa
721 aagaagtcaa atatttcaga gaaaaccaag cgtttgaatg aactaaaatt gtcggtggtt
781 tctttgaaag aaatacaaga gagtttgaaa acaaaaattg tggattctcc agagaagtta
841 aagaattata aagaaaaaat gaaagatacg gtccagaagc ttaaaaatgc cagacaagaa
901 gtggtggaga aatatgaaat ctatggagac tcagttgact gcctgccttc atgtcagttg
961 gaagtgcagt tatatcaaaa gaaaatacag gacctttcag ataataggga aaaattagcc
1021 agtatcttaa aggagagcct gaacttggag gaccaaattg agagtgatga gtcagaactg
1081 aagaaattga agactgaaga aaattcgttc aaaagactga tgattgtgaa gaaggaaaaa
1141 cttgccacag cacaattcaa aataaataag aagcatgaag atgttaagca atacaaacgc
1201 acagtaattg aggattgcaa taaagttcaa gaaaaaagag gtgctgtcta tgaacgagta
1261 accacaatta atcaagaaat ccaaaaaatt aaacttggaa ttcaacaact aaaagatgct
1321 gctgaaaggg agaaactgaa gtcccaggaa atatttctaa acttgaaaac tgctttggag
1381 aaataccacg acggtattga aaaggcagca gaggactcct atgctaagat agatgagaag
1441 acagctgaac tgaagaggaa gatgttcaaa atgtcaacct gattaacaaa attacatgtc
1501 tttttgtaaa tggcttgcca tcttttaatt ttctatttag aaagaaaagt tgaagcgaat
1561 ggaagtatca gaagtaccaa ataatgttgg cttcatcagt ttttatacac tctcataagt
1621 agttaataag atgaatttaa tgtaggcttt tattaattta taattaaaat aacttgtgca
1681 gctattcatg tctctactct gccccttgtt gtaaatagtt tgagtaaaac aaaactagtt
1741 acctttgaaa tatatatatt tttttctgaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa
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