LOCUS BC008207 1919 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens nuclear assembly factor 1 homolog (S. cerevisiae),
mRNA (cDNA clone MGC:5406 IMAGE:3447276), complete cds.
ACCESSION BC008207
VERSION BC008207.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1919)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1919)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (22-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: c Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19923910.
FEATURES Location/Qualifiers
source 1..1919
/db_xref="H-InvDB:HIT000033676"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5406 IMAGE:3447276"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_10"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1919
/gene="NAF1"
/db_xref="GeneID:92345"
/db_xref="HGNC:HGNC:25126"
CDS 195..1679
/gene="NAF1"
/codon_start=1
/product="nuclear assembly factor 1 homolog (S.
cerevisiae)"
/protein_id="AAH08207.1"
/db_xref="GeneID:92345"
/db_xref="HGNC:HGNC:25126"
/translation="MEVVEAAAAQLETLKFNGTDFGVGEGPAAPSPGSAPVPGTQPPL
QSFEGSPDAGQTVEVKPAGEQPLQPVLNAVAAGTPAPQPQPPAESPACGDCVTSPGAA
EPARAPDSLETSDSDSDSDSETDSDSSSSSSSSSSSSSSSSSSCISLPPVLSDGDDDL
QVEKENKNFPLKTKDELLLNELPSVEELTIILPEDIELKPLGMVSSIIEQLVIIESMT
NLPPVNEETVIFKSDRQAAGKIFEIFGPVAHPFYVLRFNSSDHIESKGIKIKETMYFA
PSMKDFTQYIFTEKLKQDKGSDASWKNDQEPPPEALDFSDDEKEKEAKQRKKSQIQGR
KKLKSEFNEPGEDFTEVHQNWNAHSSASEHAKGYRNREFTRGFSRARYPRSCHGRPPP
QHFYNSEHMVSQETSGFPSQRQNNPIMPQYPFPLPVFDMHNFPLRPPPPPPPPPVNMG
WATPNMAAHPLLNLPYSLPPPPPPPPLPPPPSSGDSNSHFGPYY"
BASE COUNT 525 a 455 c 425 g 514 t
ORIGIN
1 cggaaagcgt tgctgcgtaa atggcggggg cgtgtctttt ggctcctccg cgtgtagtta
61 cctgagaaac gcgggaagtt gggcccaggc agtgttgctg cggttgccta agttgtttta
121 ctatttctgg agagagccgt gagcttgtcc aggggcccca atcctgaggc cgacccggtt
181 tctggcgcgg tgcgatggag gtagtggagg ccgccgccgc tcagctggaa actctgaaat
241 tcaatggcac cgactttgga gttggggaag gtccggcggc tccgtctccg ggctctgccc
301 ctgtgccagg gacacagccg ccgctacagt cgtttgaggg gtccccggac gctgggcaga
361 ccgtggaggt taagcctgcc ggggagcagc ctctgcagcc cgttctgaac gccgtcgcgg
421 ccgggacccc ggcgccgcag ccacagccac cggctgaatc gccggcctgc ggagactgcg
481 tcacctcccc aggagccgca gagcctgcgc gggcgccgga ctccttggag acctcggact
541 cggattcgga ctcggacagt gaaacagatt cagatagttc aagttcatcg tcttcctctt
601 catcttcctc atcgtcgtct tcttcctctt gtatatcact tcctccagtg ctgtcagatg
661 gagatgatga tttacaagtt gagaaggaaa ataagaattt tcctcttaaa acaaaagatg
721 aattacttct taatgaactg ccttctgttg aagaactcac tattattctg cctgaagata
781 ttgagttaaa gcctcttggg atggtttcaa gtattattga acaactagta ataattgaat
841 ctatgactaa cctacctcca gttaatgagg agactgtaat ttttaaaagt gatcgacaag
901 cagcaggaaa gatattcgag atatttggac ctgttgcaca tccattttat gtgttacggt
961 ttaattcttc agatcacatt gagagtaaag gtattaaaat aaaggagact atgtattttg
1021 ctccatcaat gaaagatttc actcaatata tattcacaga aaaactaaaa caggataagg
1081 gatcagatgc atcatggaag aatgatcagg aaccaccacc agaggcctta gattttagtg
1141 atgatgaaaa agagaaggaa gccaaacaga ggaaaaaatc tcagattcaa ggccggaaaa
1201 aactcaaatc tgaatttaat gagcctggtg aagattttac tgaagtacat cagaattgga
1261 atgctcatag ctctgcttca gagcatgcaa aaggatatcg taacagagaa ttcacacgag
1321 gattttccag ggccagatac cctcgatctt gccatggcag gcctccacct cagcatttct
1381 ataactcaga acatatggta tctcaggaga cttcaggatt tccttctcag agacaaaata
1441 atcccattat gccacaatac ccctttcctc ttccagtgtt tgacatgcat aattttcccc
1501 tccgccctcc acccccacca ccacccccac ctgtaaacat gggttgggct acaccaaaca
1561 tggctgctca tccattactt aacttaccat actccctacc cccaccccct ccccctccac
1621 cactgcctcc tccaccctct tctggagata gtaattctca ttttggacct tactattagg
1681 tgactatgca tttccagaga aatgtggact tttcatatta tgtggtaagg attttttttt
1741 tttcaagagt gattatggag ctagattttt aaaaacactg taaaatacta aatgattcct
1801 tctgttgtaa gttgatatat atttgtaacc tttgtgaaat tgtattcata tgaaaatgtc
1861 agctcaaatt cttgggagaa cattaaatta tgtaatattt aattaaaaaa aaaaaaaaa
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