LOCUS BC008183 994 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens 5', 3'-nucleotidase, cytosolic, mRNA (cDNA clone
MGC:16998 IMAGE:4183256), complete cds.
ACCESSION BC008183
VERSION BC008183.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 994)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 994)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (22-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: l Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7657032.
FEATURES Location/Qualifiers
source 1..994
/db_xref="H-InvDB:HIT000033661"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:16998 IMAGE:4183256"
/tissue_type="Brain, glioblastoma with EGFR amplification"
/clone_lib="NCI_CGAP_Brn64"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..994
/gene="NT5C"
/gene_synonym="cdN"
/gene_synonym="DNT"
/gene_synonym="dNT-1"
/gene_synonym="DNT1"
/gene_synonym="P5N2"
/gene_synonym="PN-I"
/gene_synonym="PN-II"
/gene_synonym="UMPH2"
/db_xref="GeneID:30833"
/db_xref="MIM:191720"
CDS 37..390
/gene="NT5C"
/gene_synonym="cdN"
/gene_synonym="DNT"
/gene_synonym="dNT-1"
/gene_synonym="DNT1"
/gene_synonym="P5N2"
/gene_synonym="PN-I"
/gene_synonym="PN-II"
/gene_synonym="UMPH2"
/codon_start=1
/product="NT5C protein"
/protein_id="AAH08183.1"
/db_xref="GeneID:30833"
/db_xref="MIM:191720"
/translation="MARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGF
LAREQYRALRPDLADKVASVYEAPGFFLDLEPIPGALDAVREMNDLPDPLLKYHHCVG
EKVWLPRPYSARGAA"
BASE COUNT 225 a 296 c 321 g 152 t
ORIGIN
1 cagaccccga gagctcgcag ctccggcccg gcggcgatgg cgcggagcgt gcgcgtgctg
61 gtggacatgg acggcgtcct ggccgacttc gaggccggcc tcctgcgggg cttccgccgc
121 cgcttccctg aggagccgca cgtgccgctg gagcagcgcc gcggcttcct ggcccgcgag
181 cagtaccgcg ccctgcggcc cgacctggcg gataaagtgg ccagtgtgta cgaagccccg
241 ggctttttcc tggacctgga gcccatcccg ggagccttgg acgctgtgcg ggagatgaac
301 gacctaccgg accccctgct gaagtaccac cactgtgtgg gtgagaaggt gtggctgccc
361 cgcccctact ccgcacgtgg agctgcctaa atcctgattt taaaaagaaa ccaccagaaa
421 cccagaagta ccgctgggtg gagcagcacc tggggcccca gttcgtagaa cgaattatcc
481 tgacaaggga caagacggtg gtcttggggg acctgctcat tgatgacaag gacacagttc
541 gaggccagga ggagacccca agctgggagc acatcttgtt cacctgctgc cacaatcggc
601 acctggtcct gcccccgaca aggagacggc tgctctcctg gagtgacaac tggagggaga
661 tcttagatag caagcgcgga gctgcgcagc gggaatgagc ggggatgccg cgggcagcag
721 ctggagctaa aggaagggca ggcccacagg ggccaccgca gagccgagtc ggggcggcat
781 cgtgctggtg cctctggccc cgtggagtgg agcaggcaga taccgttaag cgctgtgcta
841 ccgggcccca ggcccagcca cccggtacct cccgagaggc tgtccctgga ccctggctgg
901 catggaaata cagtgggaaa accagtcggg acctttaata aaagaccttg gctttctaaa
961 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
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