LOCUS BC008040 1815 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens sphingosine kinase 1, mRNA (cDNA clone MGC:15041
IMAGE:3831657), complete cds.
ACCESSION BC008040
VERSION BC008040.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1815)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1815)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (21-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC008040.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 24 Row: n Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361087.
FEATURES Location/Qualifiers
source 1..1815
/db_xref="H-InvDB:HIT000033607"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15041 IMAGE:3831657"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1815
/gene="SPHK1"
/gene_synonym="SPHK"
/db_xref="GeneID:8877"
/db_xref="MIM:603730"
CDS 330..1484
/gene="SPHK1"
/gene_synonym="SPHK"
/codon_start=1
/product="SPHK1 protein"
/protein_id="AAH08040.1"
/db_xref="GeneID:8877"
/db_xref="MIM:603730"
/translation="MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAE
AEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQ
KPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASG
LRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRV
GSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAP
MGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDG
KGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL"
misc_feature 402..1400
/gene="SPHK1"
/gene_synonym="SPHK"
/note="LCB5; Region: Sphingosine kinase and enzymes
related to eukaryotic diacylglycerol kinase [Lipid
metabolism / General function prediction only]"
/db_xref="CDD:COG1597"
BASE COUNT 352 a 549 c 587 g 327 t
ORIGIN
1 gagaggccgg ggagtccgct ccagcggggc gctccagtcc ctcagacgtg ggctgagctt
61 gggacgagct gcgttccgcc ccaggccact gtagggaacg gcggtggcgc ctccccagca
121 aaccggaccg actgggtcca gccgccgcag ggaatgacgc cggtgctcct acagccacgg
181 ctccgggcgg ggaaggcgag ccccacagcc ggccctgcga cgcccgcctg ggcagcaccg
241 ataaggagct gaaggcagga gccgccgcca cgggcagcgc ccccacagcg ccagggaccc
301 cctggcagcg ggagccgcgg gtcgaggtta tggatccagc gggcggcccc cggggcgtgc
361 tcccgcggcc ctgccgcgtg ctggtgctgc tgaacccgcg cggcggcaag ggcaaggcct
421 tgcagctctt ccggagtcac gtgcagcccc ttttggctga ggctgaaatc tccttcacgc
481 tgatgctcac tgagcggcgg aaccacgcgc gggagctggt gcggtcggag gagctgggcc
541 gctgggacgc tctggtggtc atgtctggag acgggctgat gcacgaggtg gtgaacgggc
601 tcatggagcg gcctgactgg gagaccgcca tccagaagcc cctgtgtagc ctcccagcag
661 gctctggcaa cgcgctggca gcttccttga accattatgc tggctatgag caggtcacca
721 atgaagacct cctgaccaac tgcacgctat tgctgtgccg ccggctgctg tcacccatga
781 acctgctgtc tctgcacacg gcttcggggc tgcgcctctt ctctgtgctc agcctggcct
841 ggggcttcat tgctgatgtg gacctagaga gtgagaagta tcggcgtctg ggggagatgc
901 gcttcactct gggcaccttc ctgcttctgg cagccctgcg cacctaccgc ggccgactgg
961 cctacctccc tgtaggaaga gtgggttcca agacacctgc ctcccccgtt gtggtccagc
1021 agggcccggt agatgcacac cttgtgccac tggaggagcc agtgccctct cactggacag
1081 tggtgcccga cgaggacttt gtgctagtcc tggcactgct gcactcgcac ctgggcagtg
1141 agatgtttgc tgcacccatg ggccgctgtg cagctggcgt catgcatctg ttctacgtgc
1201 gggcgggagt gtctcgtgcc atgctgctgc gcctcttcct ggccatggag aagggcaggc
1261 atatggagta tgaatgcccc tacttggtat acgtgcccgt ggtcgccttc cgcttggagc
1321 ccaaggatgg gaaaggtgtg tttgcagtgg atggggaatt gatggttagc gaggccgtgc
1381 agggccaggt gcacccaaac tacttctgga tggtcagcgg ttgcgtggag cccccgccca
1441 gctggaagcc ccagcagatg ccaccgccag aagagccctt atgacccctg ggccgcgctg
1501 tgccttagtg tctacttgca ggacccttcc tccttcccta gggctgcagg gcctgtccac
1561 agctcctgtg ggggtggagg agactcctct ggagaagggt gagaaggtgg aggctatgct
1621 ttggggggac aggccagaat gaagtcctgg gtcaggagcc cagctggctg ggcccagctg
1681 cctatgtaag gccttctagt ttgttctgag accccccccc cacgaaccaa atccaaataa
1741 agtgacattc ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1801 aaaaaaaaaa aaaaa
//