LOCUS BC007769 1251 bp mRNA linear HUM 24-JAN-2005
DEFINITION Homo sapiens eukaryotic translation initiation factor 2-alpha
kinase 2, mRNA (cDNA clone IMAGE:4298375), partial cds.
ACCESSION BC007769
VERSION BC007769.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1251)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1251)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (11-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC007769.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 17 Row: l Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4506102.
FEATURES Location/Qualifiers
source 1..1251
/db_xref="H-InvDB:HIT000086911"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4298375"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_46"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..>1251
/gene="EIF2AK2"
/gene_synonym="EIF2AK1"
/gene_synonym="PKR"
/gene_synonym="PRKR"
/db_xref="GeneID:5610"
/db_xref="MIM:176871"
CDS 100..>1251
/gene="EIF2AK2"
/gene_synonym="EIF2AK1"
/gene_synonym="PKR"
/gene_synonym="PRKR"
/codon_start=1
/product="EIF2AK2 protein"
/protein_id="AAH07769.1"
/db_xref="GeneID:5610"
/db_xref="MIM:176871"
/translation="MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQ
VIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYI
GLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKL
AYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSN
SDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSG
GFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDP
ETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKKKKK"
BASE COUNT 440 a 210 c 272 g 329 t
ORIGIN
1 gtttgcttct ctggcggtct tcagaatcaa catccacact tccgtgatta tctgcgtgca
61 ttttggacaa agcttccaac caggatacgg gaagaagaaa tggctggtga tctttcagca
121 ggtttcttca tggaggaact taatacatac cgtcagaagc agggagtagt acttaaatat
181 caagaactgc ctaattcagg acctccacat gataggaggt ttacatttca agttataata
241 gatggaagag aatttccaga aggtgaaggt agatcaaaga aggaagcaaa aaatgccgca
301 gccaaattag ctgttgagat acttaataag gaaaagaagg cagttagtcc tttattattg
361 acaacaacga attcttcaga aggattatcc atggggaatt acataggcct tatcaataga
421 attgcccaga agaaaagact aactgtaaat tatgaacagt gtgcatcggg ggtgcatggg
481 ccagaaggat ttcattataa atgcaaaatg ggacagaaag aatatagtat tggtacaggt
541 tctactaaac aggaagcaaa acaattggcc gctaaacttg catatcttca gatattatca
601 gaagaaacct cagtgaaatc tgactacctg tcctctggtt cttttgctac tacgtgtgag
661 tcccaaagca actctttagt gaccagcaca ctcgcttctg aatcatcatc tgaaggtgac
721 ttctcagcag atacatcaga gataaattct aacagtgaca gtttaaacag ttcttcgttg
781 cttatgaatg gtctcagaaa taatcaaagg aaggcaaaaa gatctttggc acccagattt
841 gaccttcctg acatgaaaga aacaaagtat actgtggaca agaggtttgg catggatttt
901 aaagaaatag aattaattgg ctcaggtgga tttggccaag ttttcaaagc aaaacacaga
961 attgacggaa agacttacgt tattaaacgt gttaaatata ataacgagaa ggcggagcgt
1021 gaagtaaaag cattggcaaa acttgatcat gtaaatattg ttcactacaa tggctgttgg
1081 gatggatttg attatgatcc tgagaccagt gatgattctc ttgagagcag tgattatgat
1141 cctgagaaca gcaaaaatag ttcaaggtca aagactaagt gccttttcat ccaaatggaa
1201 ttctgtgata aagggacctt ggaacaatgg attgaaaaaa aaaaaaaaaa a
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