LOCUS BC007721 1985 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide,
mRNA (cDNA clone MGC:12704 IMAGE:4299734), complete cds.
ACCESSION BC007721
VERSION BC007721.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1985)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1985)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 9, 2003 this sequence version replaced BC007721.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 17 Row: m Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47132578.
FEATURES Location/Qualifiers
source 1..1985
/db_xref="H-InvDB:HIT000033400"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12704 IMAGE:4299734"
/tissue_type="Eye, normal, pigmented retinal epithelium"
/clone_lib="NIH_MGC_43"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1985
/gene="DFFA"
/gene_synonym="DFF-45"
/gene_synonym="DFF1"
/gene_synonym="ICAD"
/db_xref="GeneID:1676"
/db_xref="HGNC:HGNC:2772"
/db_xref="MIM:601882"
CDS 31..1026
/gene="DFFA"
/gene_synonym="DFF-45"
/gene_synonym="DFF1"
/gene_synonym="ICAD"
/codon_start=1
/product="DNA fragmentation factor, 45kDa, alpha
polypeptide"
/protein_id="AAH07721.1"
/db_xref="GeneID:1676"
/db_xref="HGNC:HGNC:2772"
/db_xref="MIM:601882"
/translation="MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRS
KACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGG
TAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQE
LRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGE
EVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALN
WDIKKTETVQEACEWELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT"
BASE COUNT 524 a 499 c 556 g 406 t
ORIGIN
1 cgaaggtggg caggtcccac cttgtggagg atggaggtga ccggggacgc cggggtacca
61 gaatctggcg agatccggac tctaaagccg tgtctgctgc gccgcaacta cagccgcgaa
121 cagcacggcg tggccgcctc ctgcctcgaa gacctgagga gcaaggcctg tgacattctg
181 gccattgata agtccctgac accagtcacc ctggtcctgg cagaggatgg caccatagtg
241 gatgatgacg attactttct gtgtctacct tccaatacta agtttgtggc attggctagt
301 aatgagaaat gggcatacaa caattcagat ggaggtacag cttggatttc ccaagagtcc
361 tttgatgtag atgaaacaga cagcggggca gggttgaagt ggaagaatgt ggccaggcag
421 ctgaaagaag atctgtccag catcatcctc ctatcagagg aggacctcca gatgcttgtt
481 gacgctccct gctcagacct ggctcaggaa ctacgtcaga gttgtgccac cgtccagcgg
541 ctgcagcaca cactccaaca ggtgcttgac caaagagagg aagtgcgtca gtccaagcag
601 ctcctgcagc tgtacctcca ggctttggag aaagagggca gcctcttgtc aaagcaggaa
661 gagtccaaag ctgcctttgg tgaggaggtg gatgcagtag acacgggtat cagcagagag
721 acctcctcgg acgttgcgct ggcgagccac atccttactg cactgaggga gaagcaggct
781 ccagagctga gcttatctag tcaggatttg gagttggtta ccaaggaaga ccccaaagca
841 ctggctgttg ccttgaactg ggacataaag aagacggaga ctgttcagga ggcctgtgag
901 tgggagctcg ccctgcgcct gcagcagacg cagagcttgc attctctccg gagcatctca
961 gcaagcaagg cctcaccacc tggtgacctg cagaatccta agcgagccag acaggatccc
1021 acatagcagc agcgggaagt gtgccaagga agctctgtgg cgttgtgtta ttggtagaca
1081 ccctcagcct catcatttga ctacctatgt actactctac cccctgcctt agagcacctt
1141 ccagagaagc tattccaggt ctcaacatac gccgttccac caattttttt tttagcccca
1201 ccagcttcag gacttctgcc aattttgaat gatatagctg caccaacaat atcccgcctc
1261 ctctaattac atatgatgtt ctctgttcaa aagtaattgg cagtgattgg ccaggcgcag
1321 tggctcacgc ctgtaatccc agcactggga ggccgagggg ggcggatcgt gaagtcagga
1381 gatcgagacc atcctggcta acatggtgaa gccctgtctc tactaaaaat acaaaaaaaa
1441 ttagccggcc atggtggcgg gcgcctgtaa tcccagctac ttgggaggct gaggcaggag
1501 aatggcatga acctgggagg cagagcttgc agtgagctga gattgcgcca ctgcactcca
1561 gcctgggcaa cagagcgaga ctccgtctca aaaaaaaaaa aaaaaaagta attggcagtg
1621 actatgggcg cactgcctaa catttagccc tgccccatat ggaacatgtt aaaaaaaaaa
1681 aaagccaggc cgagcgtgtt ggctcacgct tgtaatccca gcactttggg aggccgaggc
1741 gggtggatca cgaggtcagg agatcgagac catcctggct aacacagtga aacgtgtttt
1801 tactaaaagt acaaaaaact agctgggcgt ggtggcagga gcctgtagtc ccagctactc
1861 gggaggctga ggcaggagaa ttgcttgaac cggggaggca gaagttgcag tgagccgaga
1921 ttatgccact gtactccagc ctgggtgaca gagtgacact ctgtctcaaa aaaaaaaaaa
1981 aaaaa
//