LOCUS BC007226 2213 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens N-glycanase 1, mRNA (cDNA clone MGC:15182
IMAGE:2963554), complete cds.
ACCESSION BC007226
VERSION BC007226.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2213)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2213)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Louis M. Staudt, M.D., Ph.D.
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 22 Row: c Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21314689.
FEATURES Location/Qualifiers
source 1..2213
/db_xref="H-InvDB:HIT000033083"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15182 IMAGE:2963554"
/tissue_type="Lymph, Burkitt lymphoma"
/clone_lib="NIH_MGC_8"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2213
/gene="NGLY1"
/gene_synonym="FLJ11005"
/gene_synonym="FLJ12409"
/gene_synonym="PNG1"
/db_xref="GeneID:55768"
/db_xref="HGNC:HGNC:17646"
CDS 42..2006
/gene="NGLY1"
/gene_synonym="FLJ11005"
/gene_synonym="FLJ12409"
/gene_synonym="PNG1"
/codon_start=1
/product="N-glycanase 1"
/protein_id="AAH07226.1"
/db_xref="GeneID:55768"
/db_xref="HGNC:HGNC:17646"
/translation="MAAAALGSSSGSASPAVAELCQNTPETFLEASKLLLTYADNILR
NPNDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIR
DLIAIERSSRLDGSNKSHKVKSSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSSDPP
SASTVAADSAILEVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEKLSRARKLD
KGINISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAK
EVEDHYCDACQFSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRAVGFEARYVWDYT
DHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYS
CKHEEVIARRTKVKEALLRDTINGLNKQRQLFLSENRRKELLQRIIVELVEFISPKTP
KPGELGGRISGSVAWRVARGEMGLQRKETLFIPCENEKISKQLHLCYNIVKDRYVRVS
NNNQTISGWENGVWKMESIFRKVETDWHMVYLARKEGSSFAYISWKFECGSVGLKVDS
ISIRTSSQTFQTGTVEWKLRSDTAQVELTGDNSLHSYADFSGATEVILEAELSRGDGD
VAWQHTQLFRQSLNDHEENCLEIIIKFSDL"
BASE COUNT 677 a 425 c 529 g 582 t
ORIGIN
1 ggagagcgag gggcggcggc ggcgcccgct ggcgctcaag catggcggcg gcggcattgg
61 gcagctcctc aggctcggcg tccccggccg tggctgagct ctgccagaac accccggaga
121 cctttttgga ggcctccaag ctgctgctca cctatgctga caacatcctc agaaacccta
181 atgatgaaaa atatagatcc atccggattg gaaacacagc cttttctact agactcttgc
241 ctgtcagagg agctgttgaa tgtttatttg aaatgggctt tgaagaggga gaaacacatc
301 tcatctttcc taaaaaagct tcagtggagc agctgcaaaa aattcgtgac ctgattgcca
361 tagagagaag tagcagactg gatggctcaa ataagagcca caaagtaaag tcatctcagc
421 aacctgcagc cagtacccag cttcctacaa caccatcttc aaatcccagt gggttaaacc
481 agcacacaag gaaccgtcaa gggcagtcat cagatccacc atctgcttca acggttgctg
541 ctgactcagc cattctagaa gttcttcagt ccaacattca gcatgtgctg gtctatgaaa
601 atcctgctct tcaggagaaa gcgttggctt gtattccggt ccaagaacta aaaaggaaat
661 cacaagaaaa gttatcgaga gctagaaaat tggataaagg tatcaatata agtgatgagg
721 attttctttt gctggagctt ttgcactggt ttaaggaaga attttttcac tgggtgaata
781 acgttttgtg cagcaaatgt ggtggacaga ctaggtctag agatagatca ttactgccca
841 gtgatgatga gctgaagtgg ggtgcaaagg aagtggaaga tcattactgt gatgcctgcc
901 agttcagcaa tcgattccca agatataata accctgagaa acttttggaa acaagatgtg
961 gacggtgtgg cgagtgggcc aattgtttta cactgtgctg ccgagctgta gggtttgaag
1021 ctcgctatgt ttgggattac acagaccatg tctggacaga agtctattct ccttctcagc
1081 agcggtggct gcactgtgat gcatgtgaag atgtctgtga caagccactc ctttatgaaa
1141 taggatgggg caagaagctt tcctatgtca tagcattttc aaaagatgag gtagttgatg
1201 tcacttggcg atattcctgc aaacatgaag aggtgattgc cagaagaact aaggttaaag
1261 aagcattact tcgagacact attaatgggc ttaataagca gaggcaactg tttttgtcag
1321 aaaacagaag gaaagaactt ctccagagga taattgtgga gcttgttgaa tttatatctc
1381 ccaaaacccc taaacctgga gaacttgggg gaagaatatc tgggtcagtg gcttggagag
1441 tagcccgagg tgaaatgggt ctacagagaa aagaaacctt gtttattccc tgtgaaaatg
1501 agaagatttc taaacagctc cacctttgtt acaatattgt gaaagatcgt tatgttcgag
1561 tttcaaataa caatcaaacc atttctggat gggagaatgg cgtgtggaaa atggaatcta
1621 tattcagaaa agttgaaaca gactggcaca tggtatattt ggcccgaaag gaaggatcat
1681 cttttgctta tatttcctgg aagtttgagt gtgggtcagt tggcctaaaa gtagatagca
1741 tttctattag aacaagtagt caaacttttc agactggaac agtagaatgg aaattgcgat
1801 ctgatacagc acaagtagaa ctgacaggcg ataacagtct tcactcctat gctgattttt
1861 ctggtgccac tgaagttatt ttggaagcag aattaagcag aggagatggt gatgtcgctt
1921 ggcaacacac ccagctgttt agacaaagct taaatgacca tgaagaaaat tgtttggaga
1981 taattataaa attcagtgac ctttgagaac ctgaacatta tagaaaagct ggcaataatc
2041 aaggacttac tgaagtagtc tgttggttca gtgcatgctt agttggcagt taccaccctg
2101 tgctagcata tttcttttgc tagctatcca tcatgtaacc ctcatgaaaa ttatctttat
2161 acgtggacta taataaaata ttgaattaaa accttaaaaa aaaaaaaaaa aaa
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