LOCUS BC007216 3014 bp mRNA linear HUM 07-OCT-2003
DEFINITION Homo sapiens chromosome 5 open reading frame 4, mRNA (cDNA clone
MGC:14986 IMAGE:2906244), complete cds.
ACCESSION BC007216
VERSION BC007216.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3014)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3014)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 22 Row: a Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14150216.
FEATURES Location/Qualifiers
source 1..3014
/db_xref="H-InvDB:HIT000033075"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:14986 IMAGE:2906244"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3014
/gene="C5orf4"
/gene_synonym="FLJ13758"
/db_xref="GeneID:10826"
CDS 172..1173
/gene="C5orf4"
/gene_synonym="FLJ13758"
/codon_start=1
/product="C5orf4 protein"
/protein_id="AAH07216.1"
/db_xref="GeneID:10826"
/translation="MKGEAGHMLHNEKSKQEGHIWGSMRRTAFILGSGLLSFVAFWNS
VTWHLQRFWGASGYFWQAQWERLLTTFEGKEWILFFIGAIQVPCLFFWSFNGLLLVVD
TTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLKWWRDP
CRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISL
YAHPIEHAVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGYHLPFLPSPEFH
DYHHLKFNQCYGVLGVLDHLHGTDTMFKQTKAYERHVLLLGFTPLSESIPDSPKRME"
misc_feature 436..1029
/gene="C5orf4"
/gene_synonym="FLJ13758"
/note="Sterol_desat; Region: Sterol desaturase. This
family includes C-5 sterol desaturase and C-4 sterol
methyl oxidase. Members of this family are involved in
cholesterol biosynthesis and biosynthesis a plant
cuticular wax. These enzymes contain many conserved
histidine residues. Members of this family are integral
membrane proteins"
/db_xref="CDD:pfam01598"
BASE COUNT 802 a 827 c 680 g 705 t
ORIGIN
1 gcagctctgt aatgagatca ccatggccag cctcccaccc gagcaaaaac aaggttagcc
61 tgcagcagag ccgcagcagc aacagccacc aaagcggggg ctgaaaggga agagaactta
121 gatttgacct gtgcaaggac ctcagcagct tggacacagc ccactgcaga gatgaaagga
181 gaagctggac atatgctaca caatgaaaag tcaaagcagg agggacacat ctggggctct
241 atgaggagga cagctttcat cctgggctct ggacttctct catttgtggc cttctggaac
301 tcagtgacat ggcatcttca gagattttgg ggtgcttctg gctacttttg gcaagcccag
361 tgggagaggc tgctgactac atttgaaggg aaggagtgga tcctcttctt tataggtgcc
421 atccaagtgc cttgtctctt cttctggagc ttcaatgggc ttctattggt ggttgacaca
481 acaggaaaac ctaacttcat ctctcgctac cgaattcagg tcggcaagaa tgaacctgtg
541 gatcctgtga aactgcgcca gtctatccgc acagttcttt tcaaccagtg catgatatct
601 ttccccatgg tggtcttcct ctatcccttc ctcaaatggt ggagagaccc ctgccgccgt
661 gagctaccca ccttccactg gttcctcctg gagctggcca tcttcacgct gatcgaggaa
721 gtcttgttct actattcaca ccggctcctt caccacccaa cattctacaa gaaaatccac
781 aagaaacacc atgagtggac agctcccatt ggcgtgatct ctctctatgc ccaccctata
841 gagcatgcag tctccaacat gctaccggtg atagtgggcc cattagtaat gggctcccac
901 ttgtcctcca tcaccatgtg gttttccttg gccctcatca tcaccaccat ctcccactgt
961 ggctaccacc ttcccttcct gccttcgcct gaattccacg actaccacca tctcaagttc
1021 aaccagtgct atggggtgct gggtgtgctg gaccacctcc atgggactga caccatgttc
1081 aagcagacca aggcctacga gagacatgtc ctcctgctgg gcttcacccc gctctctgag
1141 agcatcccag actccccaaa gaggatggag tgagagacag cctaagtgtc atcctggctg
1201 tccctcagcc atgggatgca gacacggctt cctgattgca cctaacaatt tgcctccttc
1261 ggccacacgc cctaatgatg gcaccaccag ggtagaggga aggtcggctt cccggaaaag
1321 cagggccaag gatgaggctt tcttcaaact actgcccttg atgtccctca atgggatcag
1381 gagttagctt aagaaaaagg aaaacacagc tccccagact ggaggctggt cagagggagg
1441 agacccctgg tcctctgctg tggaaggaga ggggttcagc cccagataac tcctttgtgg
1501 cctgggcagg atgcagagaa tgacaaggct gaaaggaggg ggactggagg ctgcctggct
1561 ccagcgagag ctccttctgg gaccagaggg tgggacggcc aggtatcact ttgccccttc
1621 ctgccccaaa aggctttcac atacccgact caggccagag ccaagacacc ctggcaaatc
1681 attataggtc tcaattcatg acataccaga tgccagtcgc cacttcaccc aacacacaca
1741 caaacataca cacaccaaac actctgagtg agtggtaaag gccccgtttt aactctggcc
1801 caccgcaaac aaaaggtttc cctctgtggg ggagaaaaag aaatccagga gctcctccct
1861 ggattaaaac caacgaggtg cagaccaaac tttaacacct ttagccttat gtggaaacca
1921 aaaaccaact gctgggaaac tgtgaaaagc ccttttaccc acaaggggag gggtcaaagt
1981 tgctgccctt tggggacacc cgagacccct aattagccta tctgaatgag gaccaaaggt
2041 tagagccctc tttctccccg aagaaagagc ccggaaaaac atggcagagc aaagagcaaa
2101 atcctttctc ccccgaatgc tttaccagtt tctcagcaac atttattcaa gatgattttt
2161 accaggaacc ttatcaaagg caaaaccaca gctgcttggg ttgaagtccc catcctggcc
2221 tcctctcagc cgccagacat ggccaggaac cctgtggttc ccaagaacaa tttaaagatc
2281 actctttgat ttgaaagacc accattatca ttttactaaa ttcttatata tacttgtgcc
2341 ttttccaact ttcagttctc ttaagaaaaa catccactgt agctttataa aataccttag
2401 tatcagctag gtccaagtct ccaggcagga ctatcaaaat ggactccttc ttcctatcag
2461 ctctagaacc ccagaggact tgcccagtag gccagctgag cacttaccag gcagcatctc
2521 cctggccttc cacagtctcg cctgtctgtc tctggccttg gtggacctgg ctccctccct
2581 aggaggcttt tgccctcagc ttgaatacag ttcctggtgc atcaaagctg taagttctca
2641 gctgctggcc tgcactcatc acctcttcct acaaataaac atttggaaaa aagtccatct
2701 tcaatatgct taaagagaag ggtaggagat aaggagaaag aacagatacg gtttttttcc
2761 cctttaaggc ctttagattt tgaggtactg taaggggcct agaaggacaa aaggctttca
2821 ttccctcttc cttttggcag gcaggttatc agtccttggc agaagggccc agccctatcc
2881 ttttctgtat caaacaaaat ccttgaggtt ggtatacaag ttaaggctga aaaaaggcct
2941 taaattccca gtaaagaatg tgaaagcaag catgtaaaat aaactggtct tcatgaaaaa
3001 aaaaaaaaaa aaaa
//