LOCUS BC007195 2325 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens NCK adaptor protein 2, mRNA (cDNA clone MGC:10780
IMAGE:3608149), complete cds.
ACCESSION BC007195
VERSION BC007195.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2325)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2325)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 13 Row: o Column: 11.
FEATURES Location/Qualifiers
source 1..2325
/db_xref="H-InvDB:HIT000033062"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:10780 IMAGE:3608149"
/tissue_type="Uterus, endometrium adenocarcinoma"
/clone_lib="NIH_MGC_44"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2325
/gene="NCK2"
/gene_synonym="GRB4"
/gene_synonym="NCKbeta"
/db_xref="GeneID:8440"
/db_xref="HGNC:HGNC:7665"
/db_xref="MIM:604930"
CDS 58..1200
/gene="NCK2"
/gene_synonym="GRB4"
/gene_synonym="NCKbeta"
/codon_start=1
/product="NCK adaptor protein 2"
/protein_id="AAH07195.1"
/db_xref="GeneID:8440"
/db_xref="HGNC:HGNC:7665"
/db_xref="MIM:604930"
/translation="MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNA
ANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPAN
GSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWF
PSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEK
GETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSS
SGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFK
VQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ"
BASE COUNT 563 a 641 c 657 g 464 t
ORIGIN
1 gttctgcgtt ttgccagtca cctctcaact gtgtgccaaa gaaggactcc atgaaagatg
61 acagaagaag ttattgtgat agccaagtgg gactacaccg cccagcagga ccaggagctg
121 gacatcaaga agaacgagcg gctgtggttg ctggacgact ccaagacgtg gtggcgggtg
181 aggaacgcgg ccaacaggac gggctatgta ccgtccaact acgtggagcg gaagaacagc
241 ctgaagaagg gctccctcgt gaagaacctg aaggacacac taggcctcgg caagacgcgc
301 aggaagacca gcgcgcggga tgcgtccccc acgcccagca cggacgccga gtaccccgcc
361 aatggcagcg gcgccgaccg catctacgac ctcaacatcc cggccttcgt caagttcgcc
421 tatgtggccg agcgggagga tgagttgtcc ctggtgaagg ggtcgcgcgt caccgtcatg
481 gagaagtgca gcgacggttg gtggcggggc agctacaacg ggcagatcgg ctggttcccc
541 tccaactacg tcttggagga ggtggacgag gcggctgcgg agtccccaag cttcctgagc
601 ctgcgcaagg gcgcctcgct gagcaatggc cagggctccc gcgtgctgca tgtggtccag
661 acgctgtacc ccttcagctc agtcaccgag gaggagctca acttcgagaa gggggagacc
721 atggaggtga ttgagaagcc ggagaacgac cccgagtggt ggaaatgcaa aaatgcccgg
781 ggccaggtgg gcctcgtccc caaaaactac gtggtggtcc tcagtgacgg gcctgccctg
841 caccctgcgc acgccccaca gataagctac accgggccct cgtccagcgg gcgcttcgcg
901 ggcagagagt ggtactacgg gaacgtgacg cggcaccagg ccgagtgcgc cctcaacgag
961 cggggcgtgg agggcgactt cctcattagg gacagcgagt cctcgcccag cgacttctcc
1021 gtgtccctta aagcgtcagg gaagaacaaa cacttcaagg tgcagctcgt ggacaatgtc
1081 tactgcattg ggcagcggcg cttccacacc atggacgagc tggtggaaca ctacaaaaag
1141 gcgcccatct tcaccagcga gcacggggag aagctctacc tcgtcagggc cctgcagtga
1201 cggcgccccg gccccacact cgcctcccgg gccccacggt ggagctgccc gcccggcctt
1261 gtggcagagg ctcctcccgc ggggacggcc ccgacggctt ctctgcgagt ctctctttat
1321 gttcaggtcg cttggtcggt tcgtctccca tttgccatcc aggcctcaca cccacactcg
1381 agcccacccg gccggccagc tttagaggag gggaggagca gggcgagttc acattattcc
1441 ttttccatcg gaagtggcgc tcgtgcattc aactcgttcc cgctcatgga acccctcttt
1501 aaaaagacgc agggcacctg tgagcgcagg agcgagccta aggccaccca gcggcagcgc
1561 ccgtgtcctg ggcactcagc gtgctgggca gagcaggtgc gatggcccca gtcctagcag
1621 ccctcgccca tgtcctgtgc ccttacatgg ctcccggact gtgcagggag ccgatacgtt
1681 tgctgatagc aatactggaa ccaccgggtg cgatggcagt gaggagactg cccagtgcct
1741 ttggggctgt gcttgcaata aagaaagaat ttcctggaaa ggcagtctgc aaaagaggga
1801 accggtgact cagaaagaca gatgttttgg taatttaccc caaatgtgcc atccacatag
1861 tgctttttcc tcttgccctt cggcttgttt gaatctcaca attatgtatt taattctcaa
1921 agaaatatgt atctgtagcc gtttgttgac actaatacag atgattaagg aaaacagctg
1981 atctttgggg aagggagcta ccaacacttt atacacacac acacgtgcac acacacacac
2041 acacacacac tatatatata tatattattt acagggaaat ttttcagggt ttacaaaaga
2101 gtatgtgatt ggtagtaaga gacacacaga atgtttatga agaaattgca ttttcttttt
2161 cctttacatt tgaacttctt tatagtttaa atataacgtc ttgagatggc acattcctac
2221 gattgaagaa ggggtcttga gatcccctaa acttgcatac ccagtttttt ggatattgta
2281 ataaaaaaaa gtattatgac aagaaaaaaa aaaaaaaaaa aaaaa
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