LOCUS BC007060 1660 bp mRNA linear HUM 11-SEP-2007
DEFINITION Homo sapiens amylase, alpha 2A (pancreatic), mRNA (cDNA clone
MGC:12504 IMAGE:3949799), complete cds.
ACCESSION BC007060
VERSION BC007060.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1660)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1660)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (30-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 16 Row: c Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21359830.
FEATURES Location/Qualifiers
source 1..1660
/db_xref="H-InvDB:HIT000033019"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12504 IMAGE:3949799"
/tissue_type="Pancreas"
/clone_lib="NIH_MGC_78"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1660
/gene="AMY2A"
/gene_synonym="AMY2B"
/gene_synonym="PA"
/db_xref="GeneID:279"
/db_xref="HGNC:HGNC:477"
/db_xref="MIM:104650"
CDS 65..1600
/gene="AMY2A"
/gene_synonym="AMY2B"
/gene_synonym="PA"
/codon_start=1
/product="amylase, alpha 2A (pancreatic)"
/protein_id="AAH07060.1"
/db_xref="GeneID:279"
/db_xref="HGNC:HGNC:477"
/db_xref="MIM:104650"
/translation="MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECE
RYLAPKGFGGVQVSPPNENVAIYNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
NVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
GDIENYNDATQVRDCRLTGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMW
PGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK
LGTVIRKWNGEKMSYLKNWGEGWGFVPSDRALVFVDNHDNQRGHGAGGASILTFWDAR
LYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTT
CGNDWVCEHRWRQIRNMVIFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWS
FSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAE
SKL"
BASE COUNT 512 a 279 c 388 g 481 t
ORIGIN
1 aaaatgtgct tcttacagga atataaatag tttctggaaa ggacactgac aacttcaaag
61 caaaatgaag ttctttctgt tgcttttcac cattgggttc tgctgggctc agtattcccc
121 aaatacacaa caaggacgga catctattgt tcatctgttt gaatggcgat gggttgatat
181 tgctcttgaa tgtgagcgat atttagctcc gaagggattt ggaggggttc aggtctctcc
241 accaaatgaa aatgttgcaa tttacaaccc tttcagacct tggtgggaaa gataccaacc
301 agttagctat aaattatgca caagatctgg aaatgaagat gaatttagaa acatggtgac
361 tagatgtaac aatgttgggg ttcgtattta tgtggatgct gtaattaatc atatgtgtgg
421 taacgctgtg agtgcaggaa caagcagtac ctgtggaagt tacttcaacc ctggaagtag
481 ggactttcca gcagtcccat attctggatg ggatttcaat gatggtaaat gtaaaactgg
541 aagtggagat atcgagaact acaatgatgc tactcaggtc agagattgtc gtctgactgg
601 tcttcttgat cttgcactgg agaaggatta cgtgcgttct aagattgccg aatatatgaa
661 ccatctcatt gacattggtg ttgcagggtt cagacttgat gcttccaagc acatgtggcc
721 tggagacata aaggcaattt tggacaaact gcataatcta aacagtaact ggttccctgc
781 aggaagtaaa cctttcattt accaggaggt aattgatctg ggtggtgagc caattaaaag
841 cagtgactac tttggtaatg gccgggtgac agaattcaag tatggtgcaa aactcggcac
901 agttattcgc aagtggaatg gagagaagat gtcttactta aagaactggg gagaaggttg
961 gggtttcgta ccttctgaca gagcgcttgt ctttgtggat aaccatgaca atcaacgagg
1021 acatggggct ggaggagcct ctattcttac cttctgggat gctaggctgt acaaaatggc
1081 agttggattt atgcttgctc atccttacgg atttacacga gtaatgtcaa gctaccgttg
1141 gccaagacag tttcaaaatg gaaacgatgt taatgattgg gttgggccac caaataataa
1201 tggagtaatt aaagaagtta ctattaatcc agacactact tgtggcaatg actgggtctg
1261 tgaacatcga tggcgccaaa taaggaacat ggttattttc cgcaatgtag tggatggcca
1321 gccttttaca aattggtatg ataatgggag caaccaagtg gcttttggga gaggaaacag
1381 aggattcatt gttttcaaca atgatgactg gtcattttct ttaactttgc aaactggtct
1441 tcctgctggc acatactgtg atgtcatttc tggagataaa attaatggca attgcacagg
1501 cattaaaatt tacgtttctg atgatggcaa agctcatttt tctattagta actctgctga
1561 agatccattt attgcaattc atgctgaatc taaattgtaa aatttaaaat taaatgcatg
1621 tcctcaaaac aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
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