LOCUS BC006882 1590 bp mRNA linear ROD 15-JUL-2006
DEFINITION Mus musculus pyrroline-5-carboxylate reductase family, member 2,
mRNA (cDNA clone MGC:6271 IMAGE:2647612), complete cds.
ACCESSION BC006882
VERSION BC006882.1
KEYWORDS MGC.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 1590)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1590)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (27-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Gilbert Smith, Ph.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 5 Row: a Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19526877.
FEATURES Location/Qualifiers
source 1..1590
/organism="Mus musculus"
/mol_type="mRNA"
/strain="FVB/N"
/db_xref="taxon:10090"
/clone="MGC:6271 IMAGE:2647612"
/tissue_type="Mammary tumor. Metallothionien-TGF alpha
model. 10 month old virgin mouse. Taken by biopsy."
/clone_lib="NCI_CGAP_Mam1"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1590
/gene="Pycr2"
/db_xref="GeneID:69051"
/db_xref="MGI:MGI:1277956"
CDS 167..1129
/gene="Pycr2"
/codon_start=1
/product="pyrroline-5-carboxylate reductase family, member
2"
/protein_id="AAH06882.1"
/db_xref="GeneID:69051"
/db_xref="MGI:MGI:1277956"
/translation="MSVGFIGAGQLACALARGFTAAGVLSAHKIIASSPDMDLPTVSA
LRRMGVNLTRSNKDTVRHSDVLFLAVKPHIIPFILDEIGADVQERHIVVSCAAGVTIS
SVEKKLMAFQPAPKVIRCMTNTPVVVREGATVYATGTHALVEDGKLLEQLMSSVGFCT
EVEEDLIDAITGLSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQALLGAAKMLL
DSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEK
VSPAALKKTLLDRVKLESPTVSTLAPPSSGKLLTRNPAQGSKKE"
BASE COUNT 325 a 436 c 496 g 333 t
ORIGIN
1 cccacgcgtc cgctctggga tacttggggc ctctctagca gttcatctgg tgcatcccgg
61 tttgaaagct gttccgagca attcgggtcc atctttttcc acgcgcggac actgaccccg
121 ggatccccag agtccccgga tcggcgctcg ccgggatcct gcggccatga gcgtgggttt
181 catcggcgca ggccagctgg cctgtgctct ggcgcggggc ttcacggccg cgggcgtcct
241 gtcggctcac aagataatag ccagctcccc ggatatggac ctgcccacgg tgtctgcgct
301 caggaggatg ggtgtgaatc tgacccgaag caataaggac actgtgcggc acagcgacgt
361 cctgttcctg gctgtgaagc cccacatcat ccccttcatc ctggatgaga ttggagccga
421 cgtgcaggag aggcacatcg tggtctcctg tgcagccggg gtcaccatca gttctgtgga
481 gaagaagctg atggctttcc agccggcccc caaagtgatc cgctgcatga ccaacacgcc
541 cgtggtggtc cgagaggggg ccaccgtgta tgccacgggc acccatgctc tggtggaaga
601 cggcaagctt ttggagcagc tgatgagcag tgtgggcttc tgcacagagg tggaggaaga
661 cctcattgat gctatcaccg ggctcagcgg cagcgggcct gcctatgcat tcatggccct
721 ggacgccctg gcagatggtg gggtgaagat gggcgtgccg cggcgcctgg cggtcagact
781 gggagctcag gcgttgctgg gagcagctaa gatgctgctg gactcagagg accatccagg
841 ccagctcaag gacaacgtct gctcccctgg gggggccacc atccatgccc tgcacttcct
901 ggagagtggg ggcttccgct ctctgctcat caatgcggtt gaggcctcct gtatccgaac
961 cagagaactg cagtccatgg ctgaccaaga aaaggtgtct cctgctgccc ttaagaagac
1021 ccttctggac agagtgaagc tggagtcacc cacggtgtcc acgctggccc cacccagctc
1081 agggaaactc cttacaagaa accccgccca gggaagcaag aaggagtgag gaggactctg
1141 tagggtgtcc ctgtcccctg tagtacaggg aggggcaggc tcaggtctgg gggtgaagct
1201 gcttacatct gttcacctgg tgccaccgag tccttctcct gtcccgtggc agaaggtgct
1261 ctgaagaggc ggctgatgcc agaagtcagc atgcctctct gagagaggtg gaggccttag
1321 agttagcatc cccctcagcg gaagtctgtg gagagcttta gttagatccg aggctgcttc
1381 agattgatct ccaggtgagg tgagaggaaa cgggaatcct ctgaattaaa aacacgcaaa
1441 gaagcctgtg tcttggtgtt gcaggtggaa gtgtggggag tcatcctgtg acctcttgct
1501 taactcttct ggacaggtga cccattagaa ggtgatgttc agggctgctt taataaagat
1561 cttatttatt tttgaaaaaa aaaaaaaaag
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