LOCUS BC006516 1957 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens nucleolin, mRNA (cDNA clone MGC:3588 IMAGE:2960982),
complete cds.
ACCESSION BC006516
VERSION BC006516.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1957)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1957)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (17-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC006516.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 10 Row: k Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4885510.
FEATURES Location/Qualifiers
source 1..1957
/db_xref="H-InvDB:HIT000032892"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3588 IMAGE:2960982"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1957
/gene="NCL"
/gene_synonym="C23"
/db_xref="GeneID:4691"
/db_xref="MIM:164035"
CDS 148..1596
/gene="NCL"
/gene_synonym="C23"
/codon_start=1
/product="NCL protein"
/protein_id="AAH06516.3"
/db_xref="GeneID:4691"
/db_xref="MIM:164035"
/translation="MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGE
EVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAK
AVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDD
EDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDGKSKGIAYIEFKTEADAEKTF
EEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQE
VFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRG
SPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSE
EDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGG
RGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE"
misc_feature 922..1131
/gene="NCL"
/gene_synonym="C23"
/note="RRM; Region: RNA recognition motif"
/db_xref="CDD:smart00360"
BASE COUNT 652 a 377 c 531 g 397 t
ORIGIN
1 ctcagtcttt cgcctcagtc tcgagctctc gctggccttc gggtgtacgt gctccgggat
61 cttcagcacc cgcggccgcc atcgccgtcg cttggcttct tctggactca tctgcgccac
121 ttgtccgctt cacactccgc cgccatcatg gtgaagctcg cgaaggcagg taaaaatcaa
181 ggtgacccca agaaaatggc tcctcctcca aaggaggtag aagaagatag tgaagatgag
241 gaaatgtcag aagatgaaga agatgatagc agtggagaag aggtcgtcat acctcagaag
301 aaaggcaaga aggctgctgc aacctcagca aagaaggtgg tcgtttcccc aacaaaaaag
361 gttgcagttg ccacaccagc caagaaagca gctgtcactc caggcaaaaa ggcagcagca
421 acacctgcca agaagacagt tacaccagcc aaagcagtta ccacacctgg caagaaggga
481 gccacaccag gcaaagcatt ggtagcaact cctggtaaga agggtgctgc catcccagcc
541 aagggggcaa agaatggcaa gaatgccaag aaggaagaca gtgatgaaga ggaggatgat
601 gacagtgagg aggatgagga ggatgacgag gacgaggatg aggatgaaga tgaaattgaa
661 ccagcagcga tgaaagcagc agctgctgcc cctgcctcag aggatgagga cgatgaggat
721 gacgaagatg atgaggatgg gaaaagtaaa gggattgctt atattgaatt taagacagaa
781 gctgatgcag agaaaacctt tgaagaaaag cagggaacag agatcgatgg gcgatctatt
841 tccctgtact atactggaga gaaaggtcaa aatcaagact atagaggtgg aaagaatagc
901 acttggagtg gtgaatcaaa aactctggtt ttaagcaacc tctcctacag tgcaacagaa
961 gaaactcttc aggaagtatt tgagaaagca acttttatca aagtacccca gaaccaaaat
1021 ggcaaatcta aagggtatgc atttatagag tttgcttcat tcgaagacgc taaagaagct
1081 ttaaattcct gtaataaaag ggaaattgag ggcagagcaa tcaggctgga gttgcaagga
1141 cccaggggat cacctaatgc cagaagccag ccatccaaaa ctctgtttgt caaaggcctg
1201 tctgaggata ccactgaaga gacattaaag gagtcatttg acggctccgt tcgggcaagg
1261 atagttactg accgggaaac tgggtcctcc aaagggtttg gttttgtaga cttcaacagt
1321 gaggaggatg ccaaagctgc caaggaggcc atggaagacg gtgaaattga tggaaataaa
1381 gttaccttgg actgggccaa acctaagggt gaaggtggct tcgggggtcg tggtggaggc
1441 agaggcggct ttggaggacg aggtggtggt agaggaggcc gaggaggatt tggtggcaga
1501 ggccggggag gctttggagg gcgaggaggc ttccgaggag gcagaggagg aggaggtgac
1561 cacaagccac aaggaaagaa gacgaagttt gaatagcttc tgtccctctg ctttcccttt
1621 tccatttgaa agaaaggact ctggggtttt tactgttacc tgatcaatga cagagccttc
1681 tgaggacatt ccaagacagt atacagtcct gtggtctcct tggaaatccg tctagttaac
1741 atttcaaggg caataccgtg ttggttttga ctggatattc atataaactt tttaaagagt
1801 tgagtgatag agctaaccct tatctgtaag ttttgaattt atattgtttc atcccatgta
1861 caaaaccatt ttttcctaca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1921 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//