LOCUS BC006484 1480 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens thymidine kinase 1, soluble, mRNA (cDNA clone MGC:4394
IMAGE:2905608), complete cds.
ACCESSION BC006484
VERSION BC006484.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1480)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1480)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: o Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1480
/db_xref="H-InvDB:HIT000086677"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4394 IMAGE:2905608"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1480
/gene="TK1"
/gene_synonym="TK2"
/db_xref="GeneID:7083"
/db_xref="HGNC:HGNC:11830"
/db_xref="MIM:188300"
CDS 41..745
/gene="TK1"
/gene_synonym="TK2"
/codon_start=1
/product="thymidine kinase 1, soluble"
/protein_id="AAH06484.1"
/db_xref="GeneID:7083"
/db_xref="HGNC:HGNC:11830"
/db_xref="MIM:188300"
/translation="MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQ
IAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFF
PDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREA
AYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAA
RKLFAPQQILQCSPAN"
BASE COUNT 323 a 440 c 419 g 298 t
ORIGIN
1 cttggagagt actcgggttc gtgaacttcc cggaggcgca atgagctgca ttaacctgcc
61 cactgtgctg cctggctccc ccagcaagac ccgggggcag atccaggtga ttctcgggcc
121 gatgttctca ggaaaaagca cagagttgat gagacgcgtc cgtcgcttcc agattgctca
181 gtacaagtgc ctggtgatca agtatgccaa agacactcgc tacagcagca gcttctgcac
241 acatgaccgg aacaccatgg aggcactgcc cgcctgcctg ctccgagacg tggcccagga
301 ggccctgggc gtggctgtca taggcatcga cgaggggcag tttttccctg acatcgtgga
361 gttctgcgag gccatggcca acgccgggaa gaccgtaatt gtggctgcac tggatgggac
421 cttccagagg aagccatttg gggccatcct gaacctggtg ccgctggccg agagcgtggt
481 gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc gcctatacca agaggctcgg
541 cacagagaag gaggtcgagg tgattggggg agcagacaag taccactccg tgtgtcggct
601 ctgctacttc aagaaggcct caggccagcc tgccgggccg gacaacaaag agaactgccc
661 agtgccagga aagccagggg aagccgtggc tgccaggaag ctctttgccc cacagcagat
721 tctgcaatgc agccctgcca actgagggac ctgcgagggc cgcccgctcc cttcctgcca
781 ctgccgccta ctggacgctg ccctgcatgc tgcccagcca ctccaggagg aagtcgggag
841 gcgtggaggg tgaccacacc ttggccttct gggaactctc ctttgtgtgg ctgccccacc
901 tgccgcatgc tccctcctct cctacccact ggtctgctta aagcttccct ctcagctgct
961 gggacgatcg cccaggctgg agctggcccc gcttggtggc ctgggatctg gcacactccc
1021 tctccttggg gtgagggaca gagccccacg ctgttgacat cagcctgctt cttcccctct
1081 gcggctttca ctgctgagtt tctgttctcc ctgggaagcc tgtgccagca cctttgagcc
1141 ttggcccaca ctgaggctta ggcctctctg cctgggatgg gctcccaccc tcccctgagg
1201 atggcctgga ttcacgccct cttgtttcct tttgggctca aagcccttcc tacctctggt
1261 gatggtttcc acaggaacaa cagcatcttt caccaagatg ggtggcacca accttgctgg
1321 gacttggatc ccaggggctt atctcttcaa gtgtggagag ggcagggtcc acgcctctgc
1381 tgtagcttat gaaattaact aattgaaaat tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1441 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa acccccgggg
//