LOCUS BC006422 3385 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphofructokinase, liver, mRNA (cDNA clone MGC:12724
IMAGE:4040291), complete cds.
ACCESSION BC006422
VERSION BC006422.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3385)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3385)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 18 Row: o Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 50346003.
FEATURES Location/Qualifiers
source 1..3385
/db_xref="H-InvDB:HIT000032824"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12724 IMAGE:4040291"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_18"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3385
/gene="PFKL"
/gene_synonym="PFK-B"
/db_xref="GeneID:5211"
/db_xref="HGNC:HGNC:8876"
/db_xref="MIM:171860"
CDS 356..2839
/gene="PFKL"
/gene_synonym="PFK-B"
/codon_start=1
/product="phosphofructokinase, liver"
/protein_id="AAH06422.1"
/db_xref="GeneID:5211"
/db_xref="HGNC:HGNC:8876"
/db_xref="MIM:171860"
/translation="MCNQGRGRESSRGGLHVQGSCRGLSRSPQQETGFAKAPAGTDCF
FHCSPGSRGQGDRKEEVTSEPGGTSIMSRLGGMNAAVRAVTRMGIYVGAKVFLIYEGY
EGLVEGGENIKQANWLSVSNIIQLGGTIIGSARCKAFTTREGRRAAAYNLVQHGITNL
CVIGGDGSLTGANIFRSEWGSLLEELVAEGKISETTARTYSHLNIAGLVGSIDNDFCG
TDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLF
IPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSSYVKDLVVQRL
GFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVTLSGNQSVR
LPLMECVQMTKEVQKAMDDKRFDEATQLRGGSFENNWNIYKLLAHQKPPKEKSNFSLA
ILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGR
GGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLVEARGRYEELCI
VMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCG
YLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNEKCHDYYT
TEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREV
YRKGRVFANAPDSACVIGLKKKAVAFSPVTELKKDTDFEHRMPREQWWLSLRLMLKML
AQYRISMAAYVSGELEHVTRRTLSMDKGF"
BASE COUNT 659 a 1028 c 1094 g 604 t
ORIGIN
1 cgtgtttcgg ccgccgccat ggccgcggtg gacctggaga agctgcgggc gtcgggcgcg
61 ggcaaggcca tcggcgtcct gaccagcggc ggcgacgcgc aaggtcccct gacaagccca
121 ccaggccccc tgctgagatg gctgtgaccc tgggctgacc cgcccagtgg cacattgact
181 ccgcctggag ctggggagac cagagaggcc ctgtggttgg acggtggcct gggtgcgctg
241 ctcctgccct ctccttgccc tgcctcagct gctgcctgcc agaggcgtgg cacctcacct
301 cacacctgct ccctgctgct gagccccacg ccaagctgga gagcggatga gaagcatgtg
361 taaccagggt agaggtcgag agtcctctcg tgggggtctc catgttcaag ggagctgccg
421 aggcttgagc aggagccccc agcaggaaac tggctttgcc aaggcccccg ctgggacaga
481 ctgtttcttt cactgcagtc ctgggagccg agggcaaggg gacaggaaag aggaagtgac
541 ctcagagcct ggtggcacca gcatcatgtc caggctgggg ggcatgaacg ctgctgtccg
601 ggctgtgacg cgcatgggca tttatgtggg tgccaaagtc ttcctcatct acgagggcta
661 tgagggcctc gtggagggag gtgagaacat caagcaggcc aactggctga gcgtctccaa
721 catcatccag ctgggcggca ctatcattgg cagcgctcgc tgcaaggcct ttaccaccag
781 ggaggggcgc cgggcagcgg cctacaacct ggtccagcac ggcatcacca acctgtgcgt
841 catcggcggg gatggcagcc ttacaggtgc caacatcttc cgcagcgagt ggggcagcct
901 gctggaggag ctggtggcgg aaggtaagat ctcagagact acagcccgga cctactcgca
961 cctgaacatc gcgggcctag tgggctccat cgataacgac ttctgcggca ccgacatgac
1021 catcggcacg gactcggccc tccaccgcat catggaggtc atcgatgcca tcaccaccac
1081 tgcccagagc caccagagga ccttcgtgct ggaagtgatg ggccggcact gcgggtacct
1141 ggcgctggta tctgcactgg cctcaggggc cgactggctg ttcatccccg aggctccacc
1201 cgaggacggc tgggagaact tcatgtgtga gaggctgggt gagactcgga gccgtgggtc
1261 ccgactgaac atcatcatca tcgctgaggg tgccattgac cgcaacggga agcccatctc
1321 gtccagctac gtgaaggacc tggtggttca gaggctgggc ttcgacaccc gtgtaactgt
1381 gctgggccac gtgcagcggg gagggacgcc ctctgccttc gaccggatcc tgagcagcaa
1441 gatgggcatg gaggcggtga tggcgctgct ggaagccacg cctgacacgc cggcctgcgt
1501 ggtcaccctc tcggggaacc agtcagtgcg gctgcccctc atggagtgcg tgcagatgac
1561 caaggaagtg cagaaagcca tggatgacaa gaggtttgac gaggccaccc agctccgtgg
1621 tgggagcttc gagaacaact ggaacattta caagctcctc gcccaccaga agccccccaa
1681 ggagaagtct aacttctccc tggccatcct gaatgtgggg gccccggcgg ctggcatgaa
1741 tgcggccgtg cgctcggcgg tgcggaccgg catctcccat ggacacacag tatacgtggt
1801 gcacgatggc ttcgaaggcc tagccaaggg tcaggtgcaa gaagtaggct ggcacgacgt
1861 ggccggctgg ttggggcgtg gtggctccat gctggggacc aagaggaccc tgcccaaggg
1921 ccagctggag tccattgtgg agaacatccg catctatggt attcacgccc tgctggtggt
1981 cggtgggttt gaggcctatg aaggggtgct gcagctggtg gaggctcgcg ggcgctacga
2041 ggagctctgc atcgtcatgt gtgtcatccc agccaccatc agcaacaacg tccctggcac
2101 cgacttcagc ctgggctccg acactgctgt aaatgccgcc atggagagct gtgaccgcat
2161 caaacagtct gcctcgggga ccaagcgccg tgtgttcatc gtggagacca tggggggtta
2221 ctgtggctac ctggccaccg tgactggcat tgctgtgggg gccgacgccg cctacgtctt
2281 cgaggaccct ttcaacatcc acgacttaaa ggtcaacgtg gagcacatga cggagaagat
2341 gaagacagac attcagaggg gcctggtgct gcggaacgag aagtgccatg actactacac
2401 cacggagttc ctgtacaacc tgtactcatc agagggcaag ggcgtcttcg actgcaggac
2461 caatgtcctg ggccacctgc agcagggtgg cgctccaacc ccctttgacc ggaactatgg
2521 gaccaagctg ggggtgaagg ccatgctgtg gttgtcggag aagctgcgcg aggtttaccg
2581 caagggacgg gtgttcgcca atgccccaga ctcggcctgc gtgatcggcc tgaagaagaa
2641 ggcggtggcc ttcagccccg tcactgagct caagaaagac actgatttcg agcaccgcat
2701 gccacgggag cagtggtggc tgagcctgcg gctcatgctg aagatgctgg cacaataccg
2761 catcagtatg gccgcctacg tgtcagggga gctggagcac gtgacccgcc gcaccctgag
2821 catggacaag ggcttctgag gccagccatg cccacgcccc tccccagccc ccacccatgc
2881 cagcgcagcg ccagggctca gatggggcct gggctgttgt gtctggagcc tgcaggcagg
2941 tgggggctgc gtccctgctc agcccatccc ctgcctctat ccctggccac ctgccaggcc
3001 tccctccggc tggtgtcttg agaccagcct gccaggccct ccagcaggag gacagagtgc
3061 cctggggcat ccaccttcct gcccagggga cgtggcgctg tcggtgtttg gaggctgctg
3121 ccccctggct ttggcgcccc atgggccctc agcgtctccc catgctgggc tcactacatg
3181 ggccagccct tgctctacct ggccggtagg ctgctggcgc ctaggttgtg ttgagagggg
3241 gatgcccctg gccctgcctc actgtgacct gctcctgccc acgtgcagca cctgtcacct
3301 tttctagaaa taaaatcacc ctgactgtgg ggtgcatcgg tctccggaaa aaaaaaaaaa
3361 aaaaaaaaaa aaaaaaaaaa aaaaa
//