LOCUS BC006282 1122 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens vacuolar protein sorting 25 homolog (S. cerevisiae),
mRNA (cDNA clone MGC:10540 IMAGE:4025884), complete cds.
ACCESSION BC006282
VERSION BC006282.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1122)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1122)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC006282.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 15 Row: d Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 37059727.
FEATURES Location/Qualifiers
source 1..1122
/db_xref="H-InvDB:HIT000032724"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:10540 IMAGE:4025884"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1122
/gene="VPS25"
/gene_synonym="DERP9"
/gene_synonym="EAP20"
/gene_synonym="MGC10540"
/db_xref="GeneID:84313"
/db_xref="HGNC:HGNC:28122"
CDS 41..571
/gene="VPS25"
/gene_synonym="DERP9"
/gene_synonym="EAP20"
/gene_synonym="MGC10540"
/codon_start=1
/product="vacuolar protein sorting 25 homolog (S.
cerevisiae)"
/protein_id="AAH06282.1"
/db_xref="GeneID:84313"
/db_xref="HGNC:HGNC:28122"
/translation="MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLH
KQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWR
RPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEH
KAEIITVSDGRGVKFF"
BASE COUNT 269 a 308 c 292 g 253 t
ORIGIN
1 gtccttagcc ggtagcttcc gggtttcctg ggctactacg atggcgatga gtttcgagtg
61 gccgtggcag tatcgcttcc cacccttctt tacgttacaa ccgaatgtgg acactcggca
121 gaagcagctg gccgcctggt gctcgctggt cctgtccttc tgccgcctgc acaaacagtc
181 cagcatgacg gtgatggaag ctcaggagag cccgctcttc aacaacgtca agctacagcg
241 aaagcttcct gtggagtcga tccagattgt attagaggaa ctgaggaaga aagggaacct
301 cgagtggttg gataagagca agtccagctt cctgatcatg tggcggaggc cagaagaatg
361 ggggaaactc atctatcagt gggtttccag gagtggccag aacaactccg tctttaccct
421 gtatgaactg actaatgggg aagacacaga ggatgaggag ttccacgggc tggatgaagc
481 cactctactg cgggctctgc aggccctaca gcaggagcac aaggccgaga tcatcactgt
541 cagcgatggc cgaggcgtca agttcttcta gcagggacct gtctcccttt acttcttacc
601 tcccaccttt ccagggcttt caaaaggaga cagacccagt gtcccccaaa gactggatct
661 gtgactccac cagactcaaa aggactccag tcctgaaggc tgggacctgg ggatgggttt
721 ctcacacccc atatgtctgt cccttggata gggtgaggct gaagcaccag ggagaaaata
781 tgtgcttctt ctcgccctac ctcctttccc atcctagact gtccttgagc cagggtctgt
841 aaacctgaca ctttatatgt gttcacacat gtaagtacat acacacatgc gcctgcagca
901 catgcttctg tctcctcctc ctcccacccc tttagctgct gttgcctccc ttctcaggct
961 ggtgctggat ccttcctagg ggatggggga agccctggct gcaggcagcc ttccaggcaa
1021 tatgaagata ggaggcccac gggcctggca gtgagaggtg tggccccaca ccgatttatg
1081 atattaaaat ctcaactccc aaaaaaaaaa aaaaaaaaaa aa
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