LOCUS BC005253 980 bp mRNA linear HUM 15-FEB-2005
DEFINITION Homo sapiens kelch-like 20 (Drosophila), mRNA (cDNA clone
IMAGE:3951189), complete cds.
ACCESSION BC005253
VERSION BC005253.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 980)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 980)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (27-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 16 Row: k Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40807499
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..980
/db_xref="H-InvDB:HIT000032323"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3951189"
/tissue_type="Prostate"
/clone_lib="NIH_MGC_83"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..980
/gene="KLHL20"
/gene_synonym="KLEIP"
/gene_synonym="KLHLX"
/db_xref="GeneID:27252"
CDS 131..823
/gene="KLHL20"
/gene_synonym="KLEIP"
/gene_synonym="KLHLX"
/codon_start=1
/product="KLHL20 protein"
/protein_id="AAH05253.1"
/db_xref="GeneID:27252"
/translation="MEGKPMRRCTNIRPGETGMDVTSRCTLGDPNKLPEGVPQPARMP
YISDKHPRQTLEVINLLRKHRELCDVVLVVGAKKIYAHRVILSACSPYFRAMFTGELA
ESRQTEVVIRDIDERAMELLIDFAYTSQITVEEGNVQTLLPAACLLQLAEIQEACCEF
LKRQLDPSNCLGIRAFADTHSCRELLRIADKFTQHNFQEVSCTWCSNAPICWRAIWVH
ILLNNTCLGISC"
BASE COUNT 287 a 215 c 219 g 259 t
ORIGIN
1 gagacgcctc ctcctcctgt cacttcggga caagttaaca atcttcagat tcgttattaa
61 agcctcgtgt accctatgtg atctatcagg ttcggcttta gagtgtggtg aagggtactt
121 ttcatggtgc atggaaggaa agccaatgcg caggtgtacc aacattcgac caggagagac
181 tggaatggat gtaacaagcc gctgcaccct tggagacccc aacaaactgc cagaaggggt
241 tccccaacct gcccgcatgc cctatatctc agacaagcac cctcgacaaa ccttggaagt
301 gattaacctt ctgagaaagc accgggagct atgtgatgtg gtgctagttg tgggcgccaa
361 gaagatatat gcccatcgag tcattttgtc agcctgtagt ccctacttcc gagctatgtt
421 tacaggagaa ttggcagaga gccgtcagac agaagtagtg atccgagaca ttgacgagag
481 ggctatggaa ttactgattg actttgcgta tacctcccag ataacagtag aagagggcaa
541 tgttcagact cttctgccag ctgcttgcct cctccagctg gcagaaatac aggaagcctg
601 ctgtgaattc ttaaagagac aattagatcc ttctaactgc ctgggcattc gggcttttgc
661 tgacacacat tcatgtcgtg agttgctaag gatagcagac aagttcaccc aacataactt
721 tcaagaggtg agttgcactt ggtgttccaa tgcacccatt tgttggagag caatatgggt
781 tcacattctg ctaaataaca cttgcctggg tatttcctgc tgattctaac tcataatagc
841 aaataaataa gttattataa atcctaaacc tggcttttat tttagagggc aattttgtgt
901 tctaccagca agttaagatt tagggattta ttctccctaa ctgtattcat aaaaaaaaaa
961 aaaaaaaaaa aaaaaaaaaa
//