LOCUS BC004920 2010 bp mRNA linear HUM 12-NOV-2003
DEFINITION Homo sapiens phosphofructokinase, liver, mRNA (cDNA clone
IMAGE:3545993), partial cds.
ACCESSION BC004920
VERSION BC004920.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2010)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2010)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (21-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC004920.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: b Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..2010
/db_xref="H-InvDB:HIT000032084"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3545993"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2010
/gene="PFKL"
/db_xref="GeneID:5211"
/db_xref="MIM:171860"
CDS <1..1463
/gene="PFKL"
/codon_start=3
/product="PFKL protein"
/protein_id="AAH04920.2"
/db_xref="GeneID:5211"
/db_xref="MIM:171860"
/translation="VLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVTL
SGNQSVRLPLMECVQMTKEVQKAMDDKRFDEATQLRGGSFENNWNIYKLLAHQKPPKE
KSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHD
VAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLVEARG
RYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVE
TMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNE
KCHDYYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWL
SEKLREVYRKGRVFANAPDSACVIGLKKKAVAFSPVTELKKDTDFEHRMPREQWWLSL
RLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF"
misc_feature 3..1439
/gene="PFKL"
/note="Eukaryotic_PFK; Region: Phosphofructokinase, a key
regulatory enzyme in glycolysis, catalyzes the
phosphorylation of fructose-6-phosphate to
fructose-1,6-biphosphate. The members belong to a
subfamily of the PFKA family (cd00363) and include
eukaryotic ATP-dependent phosphofructokinases. These have
evolved from the bacterial PFKs by gene duplication and
fusion events and exhibit complex allosteric behavior"
/db_xref="CDD:cd00764"
BASE COUNT 409 a 601 c 635 g 365 t
ORIGIN
1 ctgtgctggg ccacgtgcag cggggaggga cgccctctgc cttcgaccgg atcctgagca
61 gcaagatggg catggaggcg gtgatggcgc tgctggaagc cacgcctgac acgccggcct
121 gcgtggtcac cctctcgggg aaccagtcag tgcggctgcc cctcatggag tgcgtgcaga
181 tgaccaagga agtgcagaaa gccatggatg acaagaggtt tgacgaggcc acccagctcc
241 gtggtgggag cttcgagaac aactggaaca tttacaagct cctcgcccac cagaagcccc
301 ccaaggagaa gtctaacttc tccctggcca tcctgaatgt gggggccccg gcggctggca
361 tgaatgcggc cgtgcgctcg gcggtgcgga ccggcatctc ccatggacac acagtatacg
421 tggtgcacga tggcttcgaa ggcctagcca agggtcaggt gcaagaagta ggctggcacg
481 acgtggccgg ctggttgggg cgtggtggct ccatgctggg gaccaagagg accctgccca
541 agggccagct ggagtccatt gtggagaaca tccgcatcta tggtattcac gccctgctgg
601 tggtcggtgg gtttgaggcc tatgaagggg tgctgcagct ggtggaggct cgcgggcgct
661 acgaggagct ctgcatcgtc atgtgtgtca tcccagccac catcagcaac aacgtccctg
721 gcaccgactt cagcctgggc tccgacactg ctgtaaatgc cgccatggag agctgtgacc
781 gcatcaaaca gtctgcctcg gggaccaagc gccgtgtgtt catcgtggag accatggggg
841 gttactgtgg ctacctggcc accgtgactg gcattgctgt gggggccgac gccgcctacg
901 tcttcgagga ccctttcaac atccacgact taaaggtcaa cgtggagcac atgacggaga
961 agatgaagac agacattcag aggggcctgg tgctgcggaa cgagaagtgc catgactact
1021 acaccacgga gttcctgtac aacctgtact catcagaggg caagggcgtc ttcgactgca
1081 ggaccaatgt cctgggccac ctgcagcagg gtggcgctcc aacccccttt gaccggaact
1141 atgggaccaa gctgggggtg aaggccatgc tgtggttgtc ggagaagctg cgcgaggttt
1201 accgcaaggg acgggtgttc gccaatgccc cagactcggc ctgcgtgatc ggcctgaaga
1261 agaaggcggt ggccttcagc cccgtcactg agctcaagaa agacactgat ttcgagcacc
1321 gcatgccacg ggagcagtgg tggctgagcc tgcggctcat gctgaagatg ctggcacaat
1381 accgcatcag tatggccgcc tacgtgtcag gggagctgga gcacgtgacc cgccgcaccc
1441 tgagcatgga caagggcttc tgaggccagc tatgcccacg cccctcccca gcccccaccc
1501 atgccagcgc agcgccaggg ctcagatggg gcctgggctg ttgtgtctgg agcctgcagg
1561 caggtggggg ctgcgtccct gctcagccca tcccctgcct ctatccctgg ccacctgcca
1621 ggcctccctc cggctggtgt cttgagacca gcctgccagg ccctccagca ggaggacaga
1681 gtgccctggg gcatccacct tcctgcccag gggacgtggc gctgtcggtg tttggaggct
1741 gctgccccct ggctttggcg ccccatgggc cctcagcgtc tccccatgct gggctcacta
1801 catgggccag cccttgctct acctggccgg taggctgctg gcgcctaggt tgtgttgaga
1861 gggggatgcc cctggccctg cctcactgtg acctgctcct gcccacgtgc agcacctgtc
1921 accttttcta gaaataaaat caccctgact gtggggtgca tcggtaaaaa aaaaaaaaaa
1981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//