LOCUS BC004524 2204 bp mRNA linear HUM 02-DEC-2006
DEFINITION Homo sapiens EF-hand calcium binding domain 4B, mRNA (cDNA clone
IMAGE:3531511), complete cds.
ACCESSION BC004524
VERSION BC004524.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2204)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2204)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC004524.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: n Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34147431
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2204
/db_xref="H-InvDB:HIT000032002"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3531511"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2204
/gene="EFCAB4B"
/gene_synonym="MGC4266"
/db_xref="GeneID:84766"
/db_xref="HGNC:HGNC:28657"
CDS 470..1657
/gene="EFCAB4B"
/gene_synonym="MGC4266"
/codon_start=1
/product="EF-hand calcium binding domain 4B"
/protein_id="AAH04524.1"
/db_xref="GeneID:84766"
/db_xref="HGNC:HGNC:28657"
/translation="MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQ
TSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNG
YLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQF
RMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNEL
ECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQE
LEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLE
SLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRCVGGHWPVLRAPPRSLGSE
GPV"
BASE COUNT 591 a 528 c 647 g 438 t
ORIGIN
1 gtcgggcgga gcaggcggct gcttgacagc tgagagccga gcgggcggcg cgggcaggtc
61 cacgggaagc ctacacgccc aaggtcatgc cctgaccaca gctgcccttc aggaggctgg
121 cacggagcca cctggggcca ttgggctgtt tgtcaagggt ggggcagcag caacttgcca
181 ctcttcctcc atgagcccag ccagagcctc cactgggcac ttagcacaag aaaaaccctg
241 caggagccgg atccagagcg ctgggattca gcaggagtgc caggagaatt caacacaggt
301 gtgctgcctc ctctcctgcc aggtgtgtgg ctgcacagga gtcttgctga tggtggaatt
361 caagtcacca gtggccaaag taccctccag gtgtgaagag ttgcaggtcc tccgaggagc
421 cgggagctgc aaggttctga aaaagacttc aagaaactga ctaatcgcga tggctgcccc
481 tgacgggagg gtagtctcca gaccccagag acttggtcag gggtctggcc aggggccaaa
541 ggggagtgga gcctgcctgc atcccctgga cagcctggag cagaaggaga ctcaggagca
601 aacgtcgggc cagctagtca tgctgaggaa ggcacaggag ttctttcaga cctgtgatgc
661 tgaaggcaag ggcttcatcg ccaggaagga tatgcagagg ctgcataagg agctaccgct
721 cagcctggag gaactggagg atgtgtttga tgccctggat gctgatggca atggctatct
781 gaccccacag gagttcacta ctggatttag tcacttcttc ttcagccaga ataacccaag
841 tcaggaagat gcaggtgaac aggtggccca gcgccatgaa gagaaggtgt atctgtccag
901 aggggatgag gatctgggcg acatgggcga agatgaggaa gcccagttcc ggatgctgat
961 ggacagactt ggagcccaaa aggtgttgga agatgaaagt gatgtcaagc agctctggtt
1021 gcagctgaag aaggaggaac ctcatttact gtccaacttt gaagacttcc tgaccagaat
1081 catctcccag ctccaagaag cccatgagga gaagaatgaa ctggagtgtg ccctaaaaag
1141 gaaaattgct gcttatgatg aagaaatcca gcatctctat gaggagatgg aacaacaaat
1201 caaaagtgag aaggagcagt ttctcctgaa ggacacagag aggtttcaag cccgcagtca
1261 agagctggag cagaaactgt tatgtaagga gcaggagctg gagcagctca cccagaagca
1321 gaaaaggctg gaaggtcagt gcacagccct gcatcatgac aagcatgaga ccaaggctga
1381 gaataccaag ctgaaactca ctaaccagga gctggcccgg gagctggagc ggacttcctg
1441 ggagctccag gatgctcagc agcagttgga aagcctccag caagaggcct gcaaactcca
1501 ccaagagaag gagatggaag tgtaccgtgt gacggagagt ctacagcgtg agaaggccgg
1561 gctcctcaag cagctggatt tcctaaggtg cgtcggtggg cactggcctg tgctgagagc
1621 acctcccaga agcctggggt cggaaggacc agtctgaggg tcatccttgc ctacgtgctg
1681 gaaaaacgtg cctgctgaga gagtgccctc aattcccttg gtccccagtg cagtgtttgg
1741 accaccaagt agccactcta ttatgtttgg tccgtttaca catgcgccca catccagttt
1801 attcctcctg gtggggaggc tctgccccac tcctctgagt ccctcacagg actgtgcttg
1861 gtacttgatc tcatgcgagc agagctgggt ttaatgagca tttaatctat gctagaaata
1921 ttatctcgtt cattgtcagc aaaactgtta ggtgagtagg gcagttcatt cacaggaatt
1981 tactgatggg aagttgatat tagaggaaag aagtaattta ccacaggcga cacagctact
2041 ctctgatggg gccagaatgg aaatccgtaa ctcggctgtg ttctgttcat gtttgttgaa
2101 tgagtaaatg aatcacaaac agagcaccaa gaaaaagctt tctcagcctt caaagacccc
2161 cagcaataaa ctcaaagttt actggtaaaa aaaaaaaaaa aaaa
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