LOCUS BC004441 1248 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens polymerase (RNA) II (DNA directed) polypeptide E,
25kDa, mRNA (cDNA clone MGC:4035 IMAGE:2820565), complete cds.
ACCESSION BC004441
VERSION BC004441.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1248)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1248)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: o Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14589950.
FEATURES Location/Qualifiers
source 1..1248
/db_xref="H-InvDB:HIT000031947"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4035 IMAGE:2820565"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1248
/gene="POLR2E"
/gene_synonym="hRPB25"
/gene_synonym="hsRPB5"
/gene_synonym="RPABC1"
/gene_synonym="RPB5"
/gene_synonym="XAP4"
/db_xref="GeneID:5434"
/db_xref="HGNC:HGNC:9192"
/db_xref="MIM:180664"
CDS 23..655
/gene="POLR2E"
/gene_synonym="hRPB25"
/gene_synonym="hsRPB5"
/gene_synonym="RPABC1"
/gene_synonym="RPB5"
/gene_synonym="XAP4"
/codon_start=1
/product="polymerase (RNA) II (DNA directed) polypeptide
E, 25kDa"
/protein_id="AAH04441.1"
/db_xref="GeneID:5434"
/db_xref="HGNC:HGNC:9192"
/db_xref="MIM:180664"
/translation="MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQF
GDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRA
LIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELL
ARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ"
BASE COUNT 264 a 392 c 372 g 220 t
ORIGIN
1 ggcggcggcg gcggaggctg ccatggacga cgaggaggag acgtaccggc tctggaaaat
61 ccgcaagacc atcatgcagc tgtgccacga ccgtggctat ctggtgaccc aggacgagct
121 tgaccagacc ctggaggagt tcaaagccca atttggggac aagccgagtg aggggcggcc
181 gcggcgcacg gacctcaccg tgctggtggc ccacaacgat gaccccaccg accagatgtt
241 tgtgttcttt ccagaggagc ccaaggtggg catcaagacc atcaaggtgt actgccagcg
301 catgcaggag gagaacatca cacgggctct catcgtggtg cagcagggca tgacaccctc
361 cgccaagcag tccctggtcg acatggcccc caagtacatc ctggagcagt ttctgcagca
421 ggagctgctc atcaacatca cggagcacga gctagtccct gagcacgtcg tcatgaccaa
481 ggaggaggtg acagagctgc tggcccgata taagctccga gagaaccagc tgcccaggat
541 ccaggcgggg gaccctgtgg cgcgctactt tgggataaag cgtgggcagg tggtgaagat
601 catccggccc agtgagacgg ctggcaggta catcacctac cggctggtgc agtagctacc
661 gcctgacagc ccctagaggc ggacacacag cgacccccat ccctgcagga caaacgcccc
721 tgccctgcca gaatccggcc cccacagctc tcacggctgc tgctcctctg gactccccaa
781 ggcaggtggc ctccacccac gttctcccgt cctggggtga ggcttcctgt ggcccagccc
841 gccccattca cctgtggatt tgtgcgagat gcagcctcag aaggaacaag gcccccagag
901 ggaggtcacc tgggggcagc tggtgccggg tcttcaccca gaccacgctg ggtcccctct
961 gttgggggtt tggggtccgg gtctcccacc agccactgct tcctcctggg ccctcggcct
1021 tccacccctc gtcttccctc cctcgggggc cctgatgcgt ggcggccccc acccggcctc
1081 ggctctttac tccattcaca gccgtgcacg cgctcaagcc accagggtgc gagatgccag
1141 ctctggagtt ctcggttgtt gtaggaggtt gggtgttttc aaatggtaaa gatgttttga
1201 gcaaataaat ttgcttgata cagaaaaaaa aaaaaaaaaa aaaaaaaa
//