LOCUS BC004278 3039 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ceramide kinase, mRNA (cDNA clone IMAGE:3615986),
complete cds.
ACCESSION BC004278
VERSION BC004278.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3039)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3039)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 13 Row: f Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32967302
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..3039
/db_xref="H-InvDB:HIT000031839"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3615986"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3039
/gene="CERK"
/gene_synonym="dA59H18.2"
/gene_synonym="dA59H18.3"
/gene_synonym="DKFZp434E0211"
/gene_synonym="FLJ21430"
/gene_synonym="FLJ23239"
/gene_synonym="hCERK"
/gene_synonym="KIAA1646"
/gene_synonym="LK4"
/db_xref="GeneID:64781"
/db_xref="HGNC:HGNC:19256"
CDS 832..1437
/gene="CERK"
/gene_synonym="dA59H18.2"
/gene_synonym="dA59H18.3"
/gene_synonym="DKFZp434E0211"
/gene_synonym="FLJ21430"
/gene_synonym="FLJ23239"
/gene_synonym="hCERK"
/gene_synonym="KIAA1646"
/gene_synonym="LK4"
/codon_start=1
/product="ceramide kinase"
/protein_id="AAH04278.1"
/db_xref="GeneID:64781"
/db_xref="HGNC:HGNC:19256"
/translation="MQRGPASGDPREGPPRQRREGCWGLHTCLITAARLRQSGWEHDL
GVCSGSTDDKLWKLAVCKALASLLLLKCQIPMLYIDAKCLTQPGLGAVRRKLAIRGGG
AGPGLPQDSSEAGPEVCTRGPDLSLSFLHTEFSIFIEHLVLLSQSSVNCVSDVCLLPR
SHDGSLVSSAARGLRRPEDSSRKAFLPRSPGHPSIVYYVLV"
BASE COUNT 738 a 723 c 773 g 805 t
ORIGIN
1 caaccagcag gaccagtttg acttcacttt tgttgaagtt tatcgcgtca agaaattcca
61 gtttacgtcg aagcacatgg aggatgagga cagcgacctc aaggaggggg ggaagaagcg
121 ctttgggcac atttgcagca gccacccctc ctgctgctgc accgtctcca acagctcctg
181 gaactgcgac ggggaggtcc tgcacagccc tgccatcgag gtcagagtcc actgccagct
241 ggttcgactc tttgcacgag gaattgaaga gaatccgaag ccagactcac acagctgaga
301 agccggcgtc ccgctcacaa actgggaaag tgtgaaaact atttaagata attattacag
361 accaattatg ttgatatata catttaaatg tagaaattta tttttgatag ttaaatcttg
421 attttagaag aaaacccttt tgtcaacaat tttgtgtaca tatttggcat tttcagttct
481 gtacgcatct gcgggttgca gcccacgccg cttactctca gcggatgcag ctgctcactt
541 gggggcactg gcctcttagg ttttaacgat gtcaacagtg tagtttagaa aatggcccgt
601 tagtggctct attgcaataa tgttagggac attatatgat ttccacgcag gtcacaccat
661 ctgggcctga ggtagcagtg ggtcactttg atccactttg caggacttat tctgtaacgg
721 tttgtggcca agttttggga agtggttgat tctctttgcc ttcatttcac cttcctcttc
781 gtttacggtt aggacatcgc tgcttgatcc ttacaatact gtgcaactgc aatgcaacgt
841 ggccctgctt caggtgatcc gcgggagggg cctccacgcc agcgccggga aggctgctgg
901 ggcctccaca cctgcctcat cacggcggcg aggctacgac aatccggctg ggagcatgac
961 cttggcgtct gttctgggag cacggatgat aagctctgga agctggcagt gtgtaaagca
1021 ctggcaagtt tgttactgtt aaaatgtcaa ataccaatgc tttatatcga cgcgaagtgc
1081 ttaacacagc cgggcttggg ggcagtcagg aggaagctgg ccatccgtgg aggaggggcc
1141 ggtcctggac tcccgcagga ctcctctgag gcagggcctg aagtctgtac acgtggtcca
1201 gatttgtcct tgtcttttct tcacactgag ttctctatat ttattgaaca tcttgtcctt
1261 ttaagccaga gtagtgtaaa ctgcgtctcg gatgtctgtc ttttgcctcg aagccacgat
1321 ggatcgctgg tttcctctgc agcgcgaggg ctccggcgac cagaggattc ttcccggaag
1381 gcattcctgc cgcgctcccc ggggcacccc tcaattgtgt actacgtcct tgtttagtgt
1441 gtatccgtgc ccacgtagat gatgtctgta acgtagtttt gtttgaaata tgagaatatg
1501 cggcttaaac tttgatctgt aaggagcggg gccgtggccg tttggagcac gctgtagaca
1561 ccgttcctca tgctgccggg tgggttttgc agaagctccc ttagtgattt catgtttaac
1621 aggcagcatc cattttcaga atttcctggc attgatttat attttgaagc atacaggaaa
1681 cttctcgttt cctcgtttag ccccacccag atcaggtgaa agggcagctt taatggtggt
1741 ttttatggac cacattatca gagagcactg tgcaagccaa atggttcaat aatgaatgaa
1801 aattctgggt gtaaagagta aatatgccct ggctctttct accaatgttt gctcctggtt
1861 ggaaagaaac caaagattta agacgggctg ctcttccaga ctggctgtgc ctgcctgtgc
1921 ccagcaacct gtgcagccgg cagtgtgcct ggtgtcacgc caggaggctg tggctgctgt
1981 gggccctctg gaattgtgct cctcacaaag tttccccaaa aggttcttct aagcctttat
2041 tgtccctggt aaatgtttcc cggctgggcg cggtggctca cgcctgtaat cccagcactt
2101 tgggaggccg aggcgggtgg atcacctaag gtcaggagtt tgagatcagc ctgcccaaca
2161 tggtgaaacc tcgtctctac taaaaataca caacttagcc agtcttgttg gcgcacgcct
2221 gtaatctcag ctactaggga tgctgaggca ggagaatcgc ttgaacccaa gaaagaggtg
2281 gaggttgcgg tgagccaaga ttgcgccact gcactccagc ctgggcaaac agagggagac
2341 tccatcgccc cccccaacaa aaaaaaagtt tcccatacac tggcctgccc caaaacccac
2401 taacaatttt agcaaaacag tccaggccaa agaggaagca tttcatgttc aataagaaac
2461 ccagccattc cgcatggctg gttcctgagt ggctctggtg atactctcca gccacctgct
2521 gacattcaga atctcagacc tcgggactgc tgttgcggta ccgtgtgtct gacacctgcc
2581 agcagccctt tgctatctgc gcgcaggatg ggggtgactg cccagacatt cccgctagat
2641 aggctctgat ttccggggca gcctttcaga tgcggcagac atacaacacc tgtactttag
2701 agttttaagg gaaaaaaaat cagaagtgct ggttagatag taaaaactta ggataactta
2761 gaaaggctag ttttagcttc ctttgtggct ccctggtgca aaacaattag cagttatgca
2821 atggacctga ttctagttta ttctaattaa gaagtgaggc cgggtttgac ttcgttcctg
2881 aatacaatct tgagtaactg ggaaagtctg agtgaaagga tggcctcatt ctctttctaa
2941 tcttgctggt ttcaagatta gaaaatggca ttatttgatc tgaaatgttt gagaagacac
3001 gaataaagtt acttgggcag aaaaaaaaaa aaaaaaaaa
//