LOCUS BC004220 1508 bp mRNA linear HUM 25-NOV-2003
DEFINITION Homo sapiens agrin, mRNA (cDNA clone IMAGE:3506210), partial cds.
ACCESSION BC004220
VERSION BC004220.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1508)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1508)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC004220.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: c Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1508
/db_xref="H-InvDB:HIT000086308"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3506210"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1508
/gene="AGRN"
/gene_synonym="FLJ45064"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
CDS <1..351
/gene="AGRN"
/gene_synonym="FLJ45064"
/codon_start=1
/product="AGRN protein"
/protein_id="AAH04220.2"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
/translation="LSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAP
VTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLE
DAVTKPELRPCPTP"
misc_feature 1..297
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="laminin_G; Region: Laminin G domain"
/db_xref="CDD:pfam00054"
BASE COUNT 264 a 487 c 451 g 306 t
ORIGIN
1 ctgagctaca acctgggctc ccagcccgtg gtgctgcgtt ccaccgtgcc cgtcaacacc
61 aaccgctggt tgcgggtcgt ggcacatagg gagcagaggg aaggttccct gcaggtgggc
121 aatgaggccc ctgtgaccgg ctcctccccg ctgggcgcca cgcagctgga cactgatgga
181 gccctgtggc ttgggggcct gccggagctg cccgtgggcc cagcactgcc caaggcctac
241 ggcacaggct ttgtgggctg cttgcgggat gtggtggtgg gccggcaccc gctgcacctg
301 ctggaggacg ccgtcaccaa gccagagctg cggccctgcc ccaccccatg agctggcacc
361 agagccccgc gcccgctgta attattttct atttttgtaa acttgttgct ttttgatatg
421 attttcttgc ctgagtgttg gccggaggga ctgctggccc ggcctccctt ccgtccaggc
481 agccgtgctg cagacagacc tagtgctgag ggatggacag gcgaggtggc agcgtggagg
541 gctcggcgtg gatggcagcc tcaggacaca cacccctgcc tcaaggtgct gagcccccgc
601 cttgcactgc gcctgcccca cggtgtcccc gccgggaagc agccccggct ccttaatcac
661 cctcgctccg tcaggcggga ctcgtgtccc agagaggaag gggctgctga ggtctgatgg
721 ggcccttcct ccgggtgacc ccacagggcc tttccaagcc cctatttgag ctgctccttc
781 ctgtgtgtgc tctggaccct gcctcggcct cctgcgccaa tactgtgact tccaaacaat
841 gttactgctg ggcacagctc tgcgttgctc ccgtgctgcc tgcgccagcc ccaggctgct
901 gaggagcaga ggccagacca gggccgatct gggtgtcctg accctcagct ggccctgccc
961 agccaccctg gacatgaccg tatccctctg ccacacccca ggccctgcga ggggctatcg
1021 agaggagctc actgtgggat ggggttgacc tctgccgcct gcctgggtat ctgggcctgg
1081 ccatggctgt gttcttcatg tgttgatttt atttgacccc tggagtggtg ggtctcatct
1141 ttcccatctc gcctgagagc ggctgagggc tgcctcactg caaatcctcc ccacagcgtc
1201 agtgaaagtc gtccttgtct cagaatgacc aggggccagc cagtgtctga ccaaggtcaa
1261 ggggcaggtg cagaggtggc agggatggct ccgaagccag aaatgcctta aactgcaacg
1321 tcccgtccct tccccacccc catcccatcc ccacccccag ccccagccca gtcctcctag
1381 gagcaggacc cgatgaagcg ggcggcggtg gggctgggtg ccgtgttact aactctagta
1441 tgtttctgtg tcaatcgctg tgaaataaag tctgaaaact ttaaaaaaaa aaaaaaaaaa
1501 aaaaaaaa
//