LOCUS BC004205 1710 bp mRNA linear HUM 03-OCT-2003
DEFINITION Homo sapiens sprouty homolog 2 (Drosophila), mRNA (cDNA clone
MGC:4026 IMAGE:3533337), complete cds.
ACCESSION BC004205
VERSION BC004205.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1710)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1710)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC004205.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: f Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1710
/db_xref="H-InvDB:HIT000050433"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4026 IMAGE:3533337"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1710
/gene="SPRY2"
/gene_synonym="hSPRY2"
/gene_synonym="MGC23039"
/db_xref="GeneID:10253"
/db_xref="MIM:602466"
CDS 157..1218
/gene="SPRY2"
/gene_synonym="hSPRY2"
/gene_synonym="MGC23039"
/codon_start=1
/product="SPRY2 protein"
/protein_id="AAH04205.1"
/db_xref="GeneID:10253"
/db_xref="MIM:602466"
/translation="MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLD
QIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSS
ARASLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKP
GELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTC
VCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCL
KLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRTLKNQHSIINQEYYSNEDFFCLFLI
HICNQLNSYNLGTVNRKLG"
BASE COUNT 454 a 406 c 389 g 461 t
ORIGIN
1 tgaggagatc cggtttccag cactgccttg ctccgaattg ggagacgttt gtcacctgcc
61 ttgttcgctg tggcctctat tctaaagagc ggagatcgga taaaggagtt cagatgtgtt
121 ctaagcctgc tggagtgacc acacttccaa gacctgatgg aggccagagc tcagagtggc
181 aacgggtcgc agcccttgct gcagacgccc cgtgacggtg gcagacagcg tggggagccc
241 gaccccagag acgccctcac ccagcaggta catgtcttgt ctctggatca gatcagagcc
301 atccgaaaca ccaatgagta cacagagggg cctactgtcg tcccaagacc tgggctcaag
361 cctgctcctc gcccctccac tcagcacaaa cacgagagac tccacggtct gcctgagcac
421 cgccagcctc ctaggctcca gcactcgcag gtccattctt ctgcacgagc ctctctgtcc
481 agatccataa gcacggtcag ctcagggtcg cggagcagta cgaggacaag taccagcagc
541 agctcctctg aacagagact gctaggatca tccttctcct ccgggcctgt tgctgatggc
601 ataatccggg tgcaacccaa atctgagctc aagccaggtg agcttaagcc actgagcaag
661 gaagatttgg gcctgcacgc ctacaggtgt gaggactgtg gcaagtgcaa atgtaaggag
721 tgcacctacc caaggcctct gccatcagac tggatctgcg acaagcagtg cctttgctcg
781 gcccagaacg tgattgacta tgggacttgt gtatgctgtg tgaaaggtct cttctatcac
841 tgttctaatg atgatgagga caactgtgct gacaacccat gttcttgcag ccagtctcac
901 tgttgtacac gatggtcagc catgggtgtc atgtccctct ttttgccttg tttatggtgt
961 taccttccag ccaagggttg ccttaaattg tgccaggggt gttatgaccg ggttaacagg
1021 cctggttgcc gctgtaaaaa ctcaaacaca gtttgctgca aagttcccac tgtcccccct
1081 agaactttga aaaaccaaca tagcatcatt aatcaggaat attacagtaa tgaggatttt
1141 ttctgtcttt ttttaataca catatgcaac caactaaaca gttataatct tggcactgtt
1201 aatagaaagt tgggatagtc tttgctgttt gcggtgaaat gctttttgtc catgtgccgt
1261 tttaactgat atgcttgtta gaactcagct aatggagctc aaagtatgag atacagaact
1321 tggtgaccca tgtattgcat aagctaaagc aacacagaca ctcctaggca aagtttttgt
1381 ttgtgaatag tacttgcaaa acttgtaaat tagcagatga cttttttcca ttgttttctc
1441 cagagagaat gtgctatatt tttgtatata caataatatt tgcaactgtg aaaaacaagt
1501 tgtgccatac tacatggcac agacacaaaa tattatacta atatgttgta cattcggaag
1561 aatgtgaatc aatcagtatg tttttagatt gtattttgcc ttacagaaag cctttattgt
1621 aagactctga tttccctttg gacttcatgt atattgtaca gttacagtaa aattcaacct
1681 ttattttcta aaaaaaaaaa aaaaaaaaaa
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