LOCUS BC004202 1893 bp mRNA linear HUM 08-OCT-2003
DEFINITION Homo sapiens CHK1 checkpoint homolog (S. pombe), mRNA (cDNA clone
MGC:3717 IMAGE:3530606), complete cds.
ACCESSION BC004202
VERSION BC004202.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1893)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1893)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC004202.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: g Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20127419.
FEATURES Location/Qualifiers
source 1..1893
/db_xref="H-InvDB:HIT000031777"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3717 IMAGE:3530606"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1893
/gene="CHEK1"
/gene_synonym="CHK1"
/db_xref="GeneID:1111"
/db_xref="MIM:603078"
CDS 60..1490
/gene="CHEK1"
/gene_synonym="CHK1"
/codon_start=1
/product="CHEK1 protein"
/protein_id="AAH04202.1"
/db_xref="GeneID:1111"
/db_xref="MIM:603078"
/translation="MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKR
AVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGM
PEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNR
ERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEY
SDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRP
RVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSY
IDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQC
LKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLE
FKRHFLKIKGKLIDIVSSQKVWLPAT"
misc_feature 84..854
/gene="CHEK1"
/gene_synonym="CHK1"
/note="S_TKc; Region: Serine/Threonine protein kinases,
catalytic domain"
/db_xref="CDD:smart00220"
BASE COUNT 585 a 377 c 437 g 494 t
ORIGIN
1 gcatttggat tcctgcagtg gtgggcaaag gacagtccgc cgaggtgctc ggtggagtca
61 tggcagtgcc ctttgtggaa gactgggact tggtgcaaac cctgggagaa ggtgcctatg
121 gagaagttca acttgctgtg aatagagtaa ctgaagaagc agtcgcagtg aagattgtag
181 atatgaagcg tgccgtagac tgtccagaaa atattaagaa agagatctgt atcaataaaa
241 tgctaaatca tgaaaatgta gtaaaattct atggtcacag gagagaaggc aatatccaat
301 atttatttct ggagtactgt agtggaggag agctttttga cagaatagag ccagacatag
361 gcatgcctga accagatgct cagagattct tccatcaact catggcaggg gtggtttatc
421 tgcatggtat tggaataact cacagggata ttaaaccaga aaatcttctg ttggatgaaa
481 gggataacct caaaatctca gactttggct tggcaacagt atttcggtat aataatcgtg
541 agcgtttgtt gaacaagatg tgtggtactt taccatatgt tgctccagaa cttctgaaga
601 gaagagaatt tcatgcagaa ccagttgatg tttggtcctg tggaatagta cttactgcaa
661 tgctcgctgg agaattgcca tgggaccaac ccagtgacag ctgtcaggag tattctgact
721 ggaaagaaaa aaaaacatac ctcaaccctt ggaaaaaaat cgattctgct cctctagctc
781 tgctgcataa aatcttagtt gagaatccat cagcaagaat taccattcca gacatcaaaa
841 aagatagatg gtacaacaaa cccctcaaga aaggggcaaa aaggccccga gtcacttcag
901 gtggtgtgtc agagtctccc agtggatttt ctaagcacat tcaatccaat ttggacttct
961 ctccagtaaa cagtgcttct agtgaagaaa atgtgaagta ctccagttct cagccagaac
1021 cccgcacagg tctttcctta tgggatacca gcccctcata cattgataaa ttggtacaag
1081 ggatcagctt ttcccagccc acatgtcctg atcatatgct tttgaatagt cagttacttg
1141 gcaccccagg atcctcacag aacccctggc agcggttggt caaaagaatg acacgattct
1201 ttaccaaatt ggatgcagac aaatcttatc aatgcctgaa agagacttgt gagaagttgg
1261 gctatcaatg gaagaaaagt tgtatgaatc aggttactat atcaacaact gataggagaa
1321 acaataaact cattttcaaa gtgaatttgt tagaaatgga tgataaaata ttggttgact
1381 tccggctttc taagggtgat ggattggagt tcaagagaca cttcctgaag attaaaggga
1441 agctgattga tattgtgagc agccagaagg tttggcttcc tgccacatga tcggaccatc
1501 ggctctgggg aatcctgatg gagtttcact cttgtctccc aggctggagt acaatggcat
1561 gatctcagct tactgcaacc tccgtctcct gggttcaagc gattctcctg cctcagcctt
1621 ccaagtagct gggattacag gtgcccacca ccacacctgg ctaggttttg tatttttagt
1681 agagatgggg tttttttcat gttggccagg ctgatctgga actcctgacc tcaagtgatc
1741 cacctgcctt ggcctcccaa agtgctggga ttttaggtgt gagccacctc gcctggcaag
1801 ggattctgtt cttagtcctt gaaaaaataa agttctgaat cttcaaaaaa aaaaaaaaaa
1861 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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