LOCUS BC004140 2297 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens serine peptidase inhibitor, Kunitz type 1, mRNA (cDNA
clone MGC:1726 IMAGE:2959383), complete cds.
ACCESSION BC004140
VERSION BC004140.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2297)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2297)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 2 Row: a Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32313598.
FEATURES Location/Qualifiers
source 1..2297
/db_xref="H-InvDB:HIT000031727"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1726 IMAGE:2959383"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2297
/gene="SPINT1"
/gene_synonym="HAI"
/gene_synonym="HAI1"
/gene_synonym="MANSC2"
/db_xref="GeneID:6692"
/db_xref="HGNC:HGNC:11246"
/db_xref="MIM:605123"
CDS 53..1594
/gene="SPINT1"
/gene_synonym="HAI"
/gene_synonym="HAI1"
/gene_synonym="MANSC2"
/codon_start=1
/product="serine peptidase inhibitor, Kunitz type 1"
/protein_id="AAH04140.1"
/db_xref="GeneID:6692"
/db_xref="HGNC:HGNC:11246"
/db_xref="MIM:605123"
/translation="MAPARTMARARLAPAGIPAVALWLLCTLGLQGTQAGPPPAPPGL
PAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALV
ELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGS
GIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTY
LFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICK
SFVYGGCLGNKNNYLREEECILACRGVQGPSMERRHPVCSGTCQPTQFRCSNGCCIDS
FLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWY
YNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREIPIPSTGSVEM
AVAVFLVICIVVVVAILGYCFFKNQRKDFHGHHHHPPPTPASSTVSTTEDTEHLVYNH
TTRPL"
BASE COUNT 487 a 722 c 649 g 439 t
ORIGIN
1 ggcacgggca gaggcccgcg ctctgaaggt gacccccctg gggaggaagg cgatggcccc
61 tgcgaggacg atggcccgcg cccgcctcgc cccggccggc atccctgccg tcgccttgtg
121 gcttctgtgc acgctcggcc tccagggcac ccaggccggg ccaccgcccg cgccccctgg
181 gctgcccgcg ggagccgact gcctgaacag ctttaccgcc ggggtgcctg gcttcgtgct
241 ggacaccaac gcctcggtca gcaacggagc taccttcctg gagtccccca ccgtgcgccg
301 gggctgggac tgcgtgcgcg cctgctgcac cacccagaac tgcaacttgg cgctagtgga
361 gctgcagccc gaccgcgggg aggacgccat cgccgcctgc ttcctcatca actgcctcta
421 cgagcagaac ttcgtgtgca agttcgcgcc cagggagggc ttcatcaact acctcacgag
481 ggaagtgtac cgctcctacc gccagctgcg gacccagggc tttggagggt ctgggatccc
541 caaggcctgg gcaggcatag acttgaaggt acaaccccag gaacccctgg tgctgaagga
601 tgtggaaaac acagattggc gcctactgcg gggtgacacg gatgtcaggg tagagaggaa
661 agacccaaac caggtggaac tgtggggact caaggaaggc acctacctgt tccagctgac
721 agtgactagc tcagaccacc cagaggacac ggccaacgtc acagtcactg tgctgtccac
781 caagcagaca gaagactact gcctcgcatc caacaaggtg ggtcgctgcc ggggctcttt
841 cccacgctgg tactatgacc ccacggagca gatctgcaag agtttcgttt atggaggctg
901 cttgggcaac aagaacaact accttcggga agaagagtgc attctagcct gtcggggtgt
961 gcaaggcccc tccatggaaa ggcgccatcc agtgtgctct ggcacctgtc agcccaccca
1021 gttccgctgc agcaatggct gctgcatcga cagtttcctg gagtgtgacg acacccccaa
1081 ctgccccgac gcctccgacg aggctgcctg tgaaaaatac acgagtggct ttgacgagct
1141 ccagcgcatc catttcccca gtgacaaagg gcactgcgtg gacctgccag acacaggact
1201 ctgcaaggag agcatcccgc gctggtacta caaccccttc agcgaacact gcgcccgctt
1261 tacctatggt ggttgttatg gcaacaagaa caactttgag gaagagcagc agtgcctcga
1321 gtcttgtcgc ggcatctcca agaaggatgt gtttggcctg aggcgggaaa tccccattcc
1381 cagcacaggc tctgtggaga tggctgtcgc agtgttcctg gtcatctgca ttgtggtggt
1441 ggtagccatc ttgggttact gcttcttcaa gaaccagaga aaggacttcc acggacacca
1501 ccaccaccca ccacccaccc ctgccagctc cactgtctcc actaccgagg acacggagca
1561 cctggtctat aaccacacca cccggcccct ctgagcctgg gtctcaccgg ctctcacctg
1621 gccctgcttc ctgcttgcca aggcagaggc ctgggctggg aaaaactttg gaaccagact
1681 cttgcctgtt tcccaggccc actgtgcctc agagaccagg gctccagccc ctcttggaga
1741 agtctcagct aagctcacgt cctgagaaag ctcaaaggtt tggaaggagc agaaaaccct
1801 tgggccagaa gtaccagact agatggacct gcctgcatag gagtttggag gaagttggag
1861 ttttgtttcc tctgttcaaa gctgcctgtc cctaccccat ggtgctagga agaggagtgg
1921 ggtggtgtca gaccctggag gccccaaccc tgtcctcccg agctcctctt ccatgctgtg
1981 cgcccagggc tgggaggaag gacttccctg tgtagtttgt gctgtaaaga gttgcttttt
2041 gtttatttaa tgctgtggca tgggtgaaga ggaggggaag aggcctgttt ggcctctctg
2101 tcctctcttc ctcttccccc aagattgagc tctctgccct tgatcagccc caccctggcc
2161 tagaccagca gacagagcca ggagaggctc agctgcattc cgcagccccc acccccaagg
2221 ttctccaaca tcacagccca gcccacccac tgggtaataa aagtggtttg tggaaaaaaa
2281 aaaaaaaaaa aaaaaaa
//