LOCUS BC004112 1183 bp mRNA linear HUM 11-DEC-2003
DEFINITION Homo sapiens sphingosine kinase 1, mRNA (cDNA clone IMAGE:3832587),
partial cds.
ACCESSION BC004112
VERSION BC004112.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1183)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1183)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 9, 2003 this sequence version replaced BC004112.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 14 Row: f Column: 13.
FEATURES Location/Qualifiers
source 1..1183
/db_xref="H-InvDB:HIT000086288"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3832587"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1183
/gene="SPHK1"
/gene_synonym="SPHK"
/db_xref="GeneID:8877"
/db_xref="MIM:603730"
CDS <1..864
/gene="SPHK1"
/gene_synonym="SPHK"
/codon_start=1
/product="SPHK1 protein"
/protein_id="AAH04112.2"
/db_xref="GeneID:8877"
/db_xref="MIM:603730"
/translation="ETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLL
LCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLR
LAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDF
VLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYE
CPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWK
PQQMPPPEEPL"
BASE COUNT 243 a 343 c 352 g 245 t
ORIGIN
1 gagaccgcca tccagaagcc cctgtgtagc ctcccagcag gctctggcaa cgcgctggca
61 gcttccttga accattatgc tggctatgag caggtcacca atgaagacct cctgaccaac
121 tgcacgctat tgctgtgccg ccggctgctg tcacccatga acctgctgtc tctgcacacg
181 gcttcggggc tgcgcctctt ctctgtgctc agcctggcct ggggcttcat tgctgatgtg
241 gacctagaga gtgagaagta tcggcgtctg ggggagatgc gcttcactct gggcaccttc
301 ctgcgtctgg cagccctgcg cacctaccgc ggccgactgg cctacctccc tgtaggaaga
361 gtgggttcca agacacctgc ctcccccgtt gtggtccagc agggcccggt agatgcacac
421 cttgtgccac tggaggagcc agtgccctct cactggacag tggtgcccga cgaggacttt
481 gtgctagtcc tggcactgct gcactcgcac ctgggcagtg agatgtttgc tgcacccatg
541 ggccgctgtg cagctggcgt catgcatctg ttctacgtgc gggcgggagt gtctcgtgcc
601 atgctgctgc gcctcttcct ggccatggag aagggcaggc atatggagta tgaatgcccc
661 tacttggtat acgtgcccgt ggtcgccttc cgcttggagc ccaaggatgg gaaaggtgtg
721 tttgcagtgg atggggaatt gatggttagc gaggccgtgc agggccaggt gcacccaaac
781 tacttctgga tggtcagcgg ttgcgtggag cccccgccca gctggaagcc ccagcagatg
841 ccaccgccag aagagccctt atgacccctg ggccgcgctg tgccttagtg tctacttgca
901 ggacccttcc tccttcccta gggctgcagg gcctgtccac agctcctgtg ggggtggagg
961 agactcctct ggagaagggt gagaaggtgg aggctatgct ttggggggac aggccagaat
1021 gaagtcctgg gtcaggagcc cagctggctg ggcccagctg cctatgtaag gccttctagt
1081 ttgttctgag acccccaccc cacgaaccaa atccaaataa agtgacattc ccagcctgaa
1141 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//