LOCUS BC003146 1720 bp mRNA linear HUM 16-SEP-2003
DEFINITION Homo sapiens splicing factor 3b, subunit 3, 130kDa, mRNA (cDNA
clone IMAGE:2822705), complete cds.
ACCESSION BC003146
VERSION BC003146.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1720)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1720)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (13-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: a Column: 4
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1720
/db_xref="H-InvDB:HIT000031464"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2822705"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1720
/gene="SF3B3"
/gene_synonym="KIAA0017"
/gene_synonym="RSE1"
/gene_synonym="SAP130"
/gene_synonym="SF3b130"
/db_xref="GeneID:23450"
/db_xref="MIM:605592"
CDS 15..1214
/gene="SF3B3"
/gene_synonym="KIAA0017"
/gene_synonym="RSE1"
/gene_synonym="SAP130"
/gene_synonym="SF3b130"
/codon_start=1
/product="SF3B3 protein"
/protein_id="AAH03146.1"
/db_xref="GeneID:23450"
/db_xref="MIM:605592"
/translation="MAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQW
ASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNIGEDWYVLVGVAKDLILNPRSV
AGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLL
RKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTA
SLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMN
YHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEH
PPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLED
IRTRYAF"
misc_feature 138..1037
/gene="SF3B3"
/gene_synonym="KIAA0017"
/gene_synonym="RSE1"
/gene_synonym="SAP130"
/gene_synonym="SF3b130"
/note="CPSF_A; Region: CPSF A subunit region. This family
includes a region that lies towards the C-terminus of the
cleavage and polyadenylation specificity factor (CPSF) A
(160 kDa) subunit. CPSF is involved in mRNA
polyadenylation and binds the AAUAAA conserved sequence in
pre-mRNA. CPSF has also been found to be necessary for
splicing of single-intron pre-mRNAs. The function of the
aligned region is unknown but may be involved in RNA/DNA
binding"
/db_xref="CDD:pfam03178"
BASE COUNT 439 a 419 c 436 g 426 t
ORIGIN
1 aattcggcac agggatggca gaggaaatgg tggaagcagc aggggaggat gagcgggagc
61 tggccgcaga gatggcagca gcattcctca atgaaaacct ccctgaatcc atctttggag
121 ctcccaaggc tggcaatggg cagtgggcct ctgtgatccg agtgatgaat cccattcaag
181 ggaacacact ggaccttgtc cagctggaac agaatgaggc agcttttagt gtggctgtgt
241 gcaggttttc caacattggt gaagactggt atgtgctggt gggtgtggcc aaggacctga
301 tactaaaccc ccgatctgtg gcagggggct tcgtctatac ttacaagctt gtgaacaatg
361 gggaaaaact ggagtttttg cacaagactc ctgtggaaga ggtccctgct gctattgccc
421 cattccaggg gagggtgttg attggtgtgg ggaagctgtt gcgtgtctat gacctgggaa
481 agaagaagtt actccgaaaa tgtgagaata agcatattgc caattatatc tctgggatcc
541 agactatcgg acatagggta attgtatctg atgtccaaga aagtttcatc tgggttcgct
601 acaagcgtaa tgaaaaccag cttatcatct ttgctgatga tacctacccc cgatgggtca
661 ctacagccag cctcctggac tatgacactg tggctggggc agacaagttt ggcaacatat
721 gtgtggtgag gctcccacct aacaccaatg atgaagtaga tgaggatcct acaggaaaca
781 aagccctgtg ggaccgtggc ttgctcaatg gggcctccca gaaggcagag gtgatcatga
841 actaccatgt cggggagacg gtgctgtcct tgcagaagac cacgctgatc cctggaggct
901 cagaatcact tgtctatacc accttgtctg gaggaattgg catccttgtg ccattcacgt
961 cccatgagga ccatgacttc ttccagcatg tggaaatgca cctgcggtct gaacatcccc
1021 ctctctgtgg gcgggaccac ctcagctttc gctcctacta cttccctgtg aagaatgtga
1081 ttgatggaga cctctgtgag cagttcaatt ccatggaacc caacaaacaa aagaacgtct
1141 ctgaagaact ggaccgaacc ccacccgaag tgtccaagaa actcgaggat atccggaccc
1201 gctacgcctt ctgagccctc ctttcccggt ggggcttgcc agagactgtg tgttttgttt
1261 cccccaccac catcactgcc acctggcttc tgccatgtgg caggagggtg actggataat
1321 taagactgca ttatgaaagt caacagctct ttcccctcag ctcttctcct ggaatgactg
1381 gcttcccctc aaattggcac tgagatttgc tacacttctc cccacctggt acatgataca
1441 tgaccccagg ttccagtgta gaacctgagt cccccattcc ccaaagccat ccctgcattg
1501 atatgtcttg actctcctgt ctacttttgc acacaccctt aatttttaat tggttttctt
1561 gtaaatacag ttttgtacaa tgttatctct gtgggaggaa ggaggcaggc tgtggtggga
1621 ctgggtaggg tatagtatca ctcctgagtt ccactgctct agaatctaac cagaaataga
1681 aacctagttt ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//