LOCUS BC003113 1548 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens nuclear autoantigenic sperm protein (histone-binding),
mRNA (cDNA clone MGC:2297 IMAGE:3504220), complete cds.
ACCESSION BC003113
VERSION BC003113.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1548)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1548)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 6 Row: b Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 27262627.
FEATURES Location/Qualifiers
source 1..1548
/db_xref="H-InvDB:HIT000031441"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2297 IMAGE:3504220"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1548
/gene="NASP"
/gene_synonym="DKFZp547F162"
/gene_synonym="FLB7527"
/gene_synonym="FLJ31599"
/gene_synonym="FLJ35510"
/gene_synonym="MGC19722"
/gene_synonym="MGC20372"
/gene_synonym="MGC2297"
/gene_synonym="PRO1999"
/db_xref="GeneID:4678"
/db_xref="HGNC:HGNC:7644"
/db_xref="MIM:603185"
CDS 78..1427
/gene="NASP"
/gene_synonym="DKFZp547F162"
/gene_synonym="FLB7527"
/gene_synonym="FLJ31599"
/gene_synonym="FLJ35510"
/gene_synonym="MGC19722"
/gene_synonym="MGC20372"
/gene_synonym="MGC2297"
/gene_synonym="PRO1999"
/codon_start=1
/product="nuclear autoantigenic sperm protein
(histone-binding)"
/protein_id="AAH03113.1"
/db_xref="GeneID:4678"
/db_xref="HGNC:HGNC:7644"
/db_xref="MIM:603185"
/translation="MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAK
KLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARME
NGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDETEGSEEDDKENDKTEEMPNDSVL
ENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESEN
YVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIE
NRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKAT
LVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDD
AKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC"
BASE COUNT 484 a 279 c 433 g 352 t
ORIGIN
1 gagtctctgg cgtcccaaat tgcctgtttt tctcgcaggc tctattccgt tcgctggttc
61 gccacctcag gggaacgatg gccatggagt ccacagccac tgccgccgtc gccgcggagc
121 tggtttctgc cgacaaaatt gaagatgttc ctgctccttc tacatctgca gataaagtgg
181 agagtctgga tgtggatagt gaagctaaga aactattggg tttaggacag aaacatctgg
241 tgatggggga tattccagca gctgtcaatg cattccagga agcagctagt cttttaggta
301 agaagtatgg agagacagct aatgagtgtg gagaagcctt ctttttctat gggaaatcac
361 ttctggagtt ggcaagaatg gagaatggtg tgttgggaaa cgccttggaa ggtgtgcatg
421 tggaagagga agaaggagaa aaaacagaag atgaatctct ggtagaaaat aatgataaca
481 tagatgaaac tgaaggctcg gaagaggatg ataaagaaaa tgataagact gaagaaatgc
541 caaatgattc agtccttgaa aacaagtctc ttcaagaaaa tgaggaggag gagattggga
601 acctagagct tgcctgggat atgctggatt tagcaaagat catttttaaa aggcaagaaa
661 caaaagaagc acagctttat gctgcccagg cacatcttaa actcggagaa gttagtgttg
721 aatctgaaaa ctatgtgcaa gctgtggagg agttccagtc ctgccttaac ctgcaggaac
781 agtacctgga agcccacgac cgtctccttg cagagaccca ctaccagctg ggcttggctt
841 atgggtacaa ctctcagtat gatgaggcag tggcacagtt cagcaaatct attgaagtca
901 ttgagaacag aatggctgta ctaaacgagc aggtgaagga ggctgaagga tcgtctgctg
961 aatacaagaa agaaattgag gaactaaagg aactgctacc cgaaattaga gagaagatag
1021 aagatgcaaa ggagtctcag cgtagtggga atgtagctga actggctctg aaagctactc
1081 tggtggagag ttctacttca ggtttcactc ctggtggagg aggctcttca gtctccatga
1141 ttgccagtag aaagccaaca gacggtgctt cctcatcaaa ttgtgtgact gatatttccc
1201 accttgtcag aaagaagagg aaaccagagg aagagagtcc ccggaaagat gatgcaaaga
1261 aagccaaaca agagccggag gtgaacggag gcagtgggga tgctgtcccc agtggaaatg
1321 aagtttcgga aaacatggag gaggaggctg agaatcaggc tgaaagccgg gcagcagtgg
1381 aggggacagt ggaggctgga gctacagttg aaagcactgc atgttaagag ggggcacagc
1441 cctcctccca agggaaagtg tttttgtata taatgtattt tttcactttt ggaggattct
1501 ttttgtataa cttcaataaa gattgtaagc aaaaaaaaaa aaaaaaaa
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