LOCUS BC003098 2636 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens hydroxysteroid (17-beta) dehydrogenase 4, mRNA (cDNA
clone MGC:1720 IMAGE:3502915), complete cds.
ACCESSION BC003098
VERSION BC003098.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2636)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2636)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 6 Row: n Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4504504.
FEATURES Location/Qualifiers
source 1..2636
/db_xref="H-InvDB:HIT000031426"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1720 IMAGE:3502915"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2636
/gene="HSD17B4"
/gene_synonym="MFE-2"
/db_xref="GeneID:3295"
/db_xref="HGNC:HGNC:5213"
/db_xref="MIM:601860"
CDS 68..2278
/gene="HSD17B4"
/gene_synonym="MFE-2"
/codon_start=1
/product="hydroxysteroid (17-beta) dehydrogenase 4"
/protein_id="AAH03098.1"
/db_xref="GeneID:3295"
/db_xref="HGNC:HGNC:5213"
/db_xref="MIM:601860"
/translation="MGSPLRFDGRVVLVTGAGAGLGRAYALAFAERGALVVVNDLGGD
FKGVGKGSLAADKVVEEIRRRGGKAVANYDSVEEGEKVVKTALDAFGRIDVVVNNAGI
LRDRSFARISDEDWDIIHRVHLRGSFQVTRAAWEHMKKQKYGRIIMTSSASGIYGNFG
QANYSAAKLGLLGLANSLAIEGRKSNIHCNTIAPNAGSRMTQTVMPEDLVEALKPEYV
APLVLWLCHESCEENGGLFEVGAGWIGKLRWERTLGAIVRQKNHPMTPEAVKANWKKI
CDFENASKPQSIQESTGSIIEVLSKIDSEGGVSANHTSRATSTATSGFAGAIGQKLPP
FSYAYTELEAIMYALGVGASIKDPKDLKFIYEGSSDFSCLPTFGVIIGQKSMMGGGLA
EIPGLSINFAKVLHGEQYLELYKPLPRAGKLKCEAVVADVLDKGSGVVIIMDVYSYSE
KELICHNQFSLFLVGSGGFGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLS
GDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAKP
VYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDLAPTSGTSAKTPSEGGKLQS
TFVFEEIGRRLKDIGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKVYQGPAKG
AADTTIILSDEDFMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL"
BASE COUNT 779 a 480 c 646 g 731 t
ORIGIN
1 ctcctgtccc gcagtcggcg tccagcggct ctgcttgttc gtgtgtgtgt cgttgcaggc
61 cttattcatg ggctcaccgc tgaggttcga cgggcgggtg gtactggtca ccggcgcggg
121 ggcaggattg ggccgagcct atgccctggc ttttgcagaa agaggagcgt tagttgttgt
181 gaatgatttg ggaggggact tcaaaggagt tggtaaaggc tccttagctg ctgataaggt
241 tgttgaagaa ataagaagga gaggtggaaa agcagtggcc aactatgatt cagtggaaga
301 aggagagaag gttgtgaaga cagccctgga tgcttttgga agaatagatg ttgtggtcaa
361 caatgctgga attctgaggg atcgttcctt tgctaggata agtgatgaag actgggatat
421 aatccacaga gttcatttgc ggggttcatt ccaagtgaca cgggcagcat gggaacacat
481 gaagaaacag aagtatggaa ggattattat gacttcatca gcttcaggaa tatatggcaa
541 ctttggccag gccaattata gtgctgcaaa gttgggtctt ctgggccttg caaattctct
601 tgcaattgaa ggcaggaaaa gcaacattca ttgtaacacc attgctccta atgcgggatc
661 acggatgact cagacagtta tgcctgaaga tcttgtggaa gccctgaagc cagagtatgt
721 ggcacctctt gtcctttggc tttgtcacga gagttgtgag gagaatggtg gcttgtttga
781 ggttggagca ggatggattg gaaaattacg ctgggagcgg actcttggag ctattgtaag
841 acaaaagaat cacccaatga ctcctgaggc agtcaaggct aactggaaga agatctgtga
901 ctttgagaat gccagcaagc ctcagagtat ccaagaatca actggcagta taattgaagt
961 tctgagtaaa atagattcag aaggaggagt ttcagcaaat catactagtc gtgcaacgtc
1021 tacagcaaca tcaggatttg ctggagctat tggccagaaa ctccctccat tttcttatgc
1081 ttatacggaa ctggaagcta ttatgtatgc ccttggagtg ggagcgtcaa tcaaggatcc
1141 aaaagatttg aaatttattt atgaaggaag ttctgatttc tcctgtttgc ccaccttcgg
1201 agttatcata ggtcagaaat ctatgatggg tggaggatta gcagaaattc ctggactttc
1261 aatcaacttt gcaaaggttc ttcatggaga gcagtactta gagttatata aaccacttcc
1321 cagagcagga aaattaaaat gtgaagcagt tgttgctgat gtcctagata aaggatccgg
1381 tgtagtgatt attatggatg tctattctta ttctgagaag gaacttatat gccacaatca
1441 gttctctctc tttcttgttg gctctggagg ctttggtgga aaacggacat cagacaaagt
1501 caaggtagct gtagccatac ctaatagacc tcctgatgct gtacttacag ataccacctc
1561 tcttaatcag gctgctttgt accgcctcag tggagactgg aatcccttac acattgatcc
1621 taactttgct agtctagcag gttttgacaa gcccatatta catggattat gtacatttgg
1681 attttctgcc aggcgtgtgt tacagcagtt tgcagataat gatgtgtcaa gattcaaggc
1741 aattaaggct cgttttgcaa aaccagtata tccaggacaa actctacaaa ctgagatgtg
1801 gaaggaagga aacagaattc attttcaaac caaggtccaa gaaactggag acattgtcat
1861 ttcaaatgca tatgtggatc ttgcaccaac atctggtact tcagctaaga caccctctga
1921 gggcgggaag cttcagagta cctttgtatt tgaggaaata ggacgccgcc taaaggatat
1981 tgggcctgag gtggtgaaga aagtaaatgc tgtatttgag tggcatataa ccaaaggcgg
2041 aaatattggg gctaagtgga ctattgacct gaaaagtggt tctggaaaag tgtaccaagg
2101 ccctgcaaaa ggtgctgctg atacaacaat catactttca gatgaagatt tcatggaggt
2161 ggtcctgggc aagcttgacc ctcagaaggc attctttagt ggcaggctga aggccagagg
2221 gaacatcatg ctgagccaga aacttcagat gattcttaaa gactacgcca agctctgaag
2281 ggcacactac actattaata aaaatggaat cattaaatac tctcttcacc caaatatgct
2341 tgattattct gcaaaagtga ttagaactaa gatgcagggg aaattgctta acattttcag
2401 atatcagata actgcagatt ttcattttct actaattttt catgtatcat tatttttaca
2461 aggaactata tataagctag cacataatta tccttctgtt cttagatctg tatcttcata
2521 ataaaaaatt ttgcccaagt cctgtttcct tagaatttgt gatagcattg ataagttgaa
2581 aggaaaatta aatcaataaa ggcctttgat acctttaaaa aaaaaaaaaa aaaaaa
//